Major Histocompatibility Complex Class II-Independent Generation of Neutralizing Antibodies against T-Cell-Dependent<i>Borrelia burgdorferi</i>Antigens Presented by Dendritic Cells: Regulation by NK and γδ T Cells

Ml, Mbow; Zeidner, Nordin S.; Rd, Gilmore; Dolan, Marc C.; Piesman, Joseph; Rg, Titus

doi:10.1128/iai.69.4.2407-2415.2001

Cited by 15 publications

(11 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Bacterial cell wall fragments have been previously detected in rats months after inoculation 51 and a recent study shows the persistence of Borrelia peptidoglycan in the synovial environment for several weeks after antibiotic treatment 17 . It is also known that antigens from several pathogens, including Borrelia, can persist in the lymph nodes in association with antigen presenting cells such as dendritic cells (DC) 52 and also with non-hematopoietic cells such as lymph node stromal cells 53,54 for at least several weeks after infection resolution 53 . DCs play a crucial role in the development of a specific T-cell mediated response to Borrelia after phagocytosis 55,56 .…”

Section: Discussionmentioning

confidence: 99%

Evaluation of pathogen specific urinary peptides in tick-borne illnesses

Magni

Almofee

Yusuf

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Mass spectrometry enhanced by nanotechnology can achieve previously unattainable sensitivity for characterizing urinary pathogen-derived peptides. We utilized mass spectrometry enhanced by affinity hydrogel particles (analytical sensitivity = 2.5 pg/mL) to study tick pathogen-specific proteins shed in the urine of patients with (1) erythema migrans rash and acute symptoms, (2) post treatment Lyme disease syndrome (PTLDS), and (3) clinical suspicion of tick-borne illnesses (TBI). Targeted pathogens were Borrelia, Babesia, Anaplasma, Rickettsia, Ehrlichia, Bartonella, Francisella, Powassan virus, tick-borne encephalitis virus, and Colorado tick fever virus. Specificity was defined by 100% amino acid sequence identity with tick-borne pathogen proteins, evolutionary taxonomic verification for related pathogens, and no identity with human or other organisms. Using a cut off of two pathogen peptides, 9/10 acute Lyme Borreliosis patients resulted positive, while we identified zero false positive in 250 controls. Two or more pathogen peptides were identified in 40% of samples from PTLDS and TBI patients (categories 2 and 3 above, n = 59/148). Collectively, 279 distinct unique tick-borne pathogen derived peptides were identified. The number of pathogen specific peptides was directly correlated with presence or absence of symptoms reported by patients (ordinal regression pseudo-R2 = 0.392, p = 0.010). Enhanced mass spectrometry is a new tool for studying tick-borne pathogen infections.

show abstract

Section: Discussionmentioning

confidence: 99%

Evaluation of pathogen specific urinary peptides in tick-borne illnesses

Magni

Almofee

Yusuf

et al. 2020

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Furthermore, elevated levels of soluble MICA have been shown to impair NK cell function in chronic HIV-1 infection [30]. MICA is also a ligand for NKG2D on γδ T cells, which have been shown to increase neutralizing antibodies in the absence of CD4 αβ T cells against vesicular stomatitis virus (VSV), foot-and-mouth disease virus, as well as Borrelia burgdorferi, the causative agent for lyme disease [31]–[33].…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Association Study on the Development of Cross-Reactive Neutralizing Antibodies in HIV-1 Infected Individuals

et al. 2013

View full text Add to dashboard Cite

Broadly neutralizing antibodies may protect against HIV-1 acquisition. In natural infection, only 10–30% of patients have cross-reactive neutralizing humoral immunity which may relate to viral and or host factors. To explore the role of host genetic markers in the formation of cross-reactive neutralizing activity (CrNA) in HIV-1 infected individuals, we performed a genome-wide association study (GWAS), in participants of the Amsterdam Cohort Studies with known CrNA in their sera. Single-nucleotide polymorphisms (SNPs) with the strongest P-values are located in the major histocompatibility complex (MHC) region, close to MICA (P = 7.68×10−7), HLA-B (P = 6.96×10−6) and in the coding region of HCP5 (P = 1.34×10−5). However, none of the signals reached genome-wide significance. Our findings underline the potential involvement of genes close or within the MHC region with the development of CrNA.

show abstract

“…Although it was not that surprising that low numbers of TCR gd T-cells are populating the prostate, since they are known to appear preferentially in association with epithelial cells, the ®nding that up to 40% of T-cells of the individual BPH-T cell lines expressed the gd TCR may be of functional signi®cance. In humans, gd T-cells have been shown to play a major role in the immune response against various infectious agents, such as mycobacteria [25] or Borrelia burgdorferi [26]. In addition, several lines of evidence suggest that gd T cells may also initiate immunity against common bacteria like Escherichia coli [27].…”

Section: Discussionmentioning

confidence: 99%