Major Histocompatibility Complex Class II Molecule-Human Immunodeficiency Virus Peptide Analysis Using a Microarray Chip

Abstract

Major histocompatibility complex (MHC) class II alleles, cell surface glycoproteins with a high degree of allelic polymorphism (8), are constitutively expressed on professional antigen-presenting cells. The peptide repertoire presented by MHC class II molecules to CD4 ϩ T cells is edited by intracellular, nonclassical MHC molecules, i.e., HLA-DM and -DO (43). MHC class II alleles exhibit four major pockets (13) to accommodate and present a broad peptide repertoire to CD4 ϩ

NN-align. An artificial neural network-based alignment algorithm for MHC class II peptide binding prediction

“…The NN-align algorithm, even though here only applied to the problem of peptide MHC class II binding prediction based on peptide data points, should be generally applicable to find subtle linear sequence motifs in large scale quantitative data sets like peptide chip data for MHC binding, TCR recognition, monoclonal antibodies etc., where the precise location of the receptor peptide interaction is not a priori known [ 34 ].…”

Peptide Microarray-Based Identification of Mycobacterium tuberculosis Epitope Binding to HLA-DRB1*0101, DRB1*1501, and DRB1*0401

“…HLA-DR monomers were incubated with the peptide microarrays as described previously (9). Briefly, MHC class II monomers were diluted to a working concentration of 1 g/ml using a binding buffer (36 mM phosphate, 14.4 mM citrate, 0.15% bovine serum albumin [BSA], 0.25% octyl beta-D-glucopyranoside, 0.02% NaN 3 , pH 5.5).…”

“…We described a peptide microarray assay that allowed us to visualize HIV peptide binding to molecularly defined MHC class II alleles (9). The assay has the major advantage that a high number of candidate peptides can be screened within a short time frame.…”

Potential of Ni(II)-NTA-Modified Poly(ethylene imine) Glycopolymers as Carrier System for Future Dendritic Cell-Based Immunotherapy

“…Due to the role of Gp41 in HIV infection, it is an important target for the development of HIV vaccination strategies. 23,24 Here, we investigated the impact of Ni(II)-NTA-modified poly(ethylene imine) dendritic glycopolymer (Ni(II)-NTA-DG) on the uptake of histidine-tagged peptide (His 6 -Gp160) by monocytes and iDCs and the localization of His 6 -Gp160 in the endosomal and lysosomal compartments of these cell subsets. Another important task is that DCs treated with polyplexes remain their immunostimulatory potential.…”