Major ginsenosides from Panax ginseng promote aerobic cellular respiration and SIRT1-mediated mitochondrial biosynthesis in cardiomyocytes and neurons

Huang, Qingxia; Lou, Tingting; Lü, Jing; Wang, Man-Ying; Chen, Xuenan; Xue, Linyuan; Tang, Xiaolei; Qi, Wenxiu; Zhang, Zepeng; Su, Hang; Jin, Wenqi; Jing, Chuanyong; Zhao, Daqing; Sun, Liwei; Li, Xiangyan

doi:10.1016/j.jgr.2022.02.002

Cited by 10 publications

(9 citation statements)

References 48 publications

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“…Previous studies have reported that SIRT1 can regulate mitochondrial functions by affecting ROS [ 48 , 51 ], which are primarily produced in mitochondria and can cause oxidative damage [ 5 ]. In our data, we found that the expression of SIRT1 and RanGap1 affected ROS ( Figure 5 A,B).…”

Section: Resultsmentioning

confidence: 99%

Systematic Insight of Resveratrol Activated SIRT1 Interactome through Proximity Labeling Strategy

Zhang

Han

et al. 2022

Antioxidants

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SIRT1 functions by regulating the modification of proteins or interacting with other proteins to form complexes. It has been widely studied and found to play significant roles in various biological processes and diseases. However, systematic studies on activated-SIRT1 interactions remain limited. Here, we present a comprehensive SIRT1 interactome under resveratrol stimulation through proximity labeling methods. Our results demonstrated that RanGap1 interacted with SIRT1 in HEK 293T cells and MCF-7 cells. SIRT1 regulated the protein level of RanGap1 and had no obvious effect on RanGap1 transcription. Moreover, the overexpression of Rangap1 increased the ROS level in MCF-7 cells, which sensitized cells to resveratrol and reduced the cell viability. These findings provide evidence that RanGap1 interacts with SIRT1 and influences intracellular ROS, critical signals for mitochondrial functions, cell proliferation and transcription. Additionally, we identified that the SIRT1-RanGap1 interaction affects downstream signals induced by ROS. Overall, our study provides an essential resource for future studies on the interactions of resveratrol-activated SIRT1. There are conflicts about the relationship between resveratrol and ROS in previous reports. However, our data identified the impact of the resveratrol-SIRT1-RanGap1 axis on intracellular ROS.

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Section: Resultsmentioning

confidence: 99%

Systematic Insight of Resveratrol Activated SIRT1 Interactome through Proximity Labeling Strategy

Zhang

Han

et al. 2022

Antioxidants

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“…It has been identi ed that Rb1 is closely related to mitochondrial dysfunction. Rb1 signi cantly increases mitochondrial content in myocardium and enhances aerobic cellular respiration by activating the SIRT1 pathway to promote mitochondrial energy metabolism and ATP synthesis, thereby protecting cardiomyocytes [30] . Rb1 induces browning of 3T3-L1 and primary white adipocytes, enhances PGC-1α expression in brown adipocytes, and thus increases mitochondrial density [31] .…”

Section: Discussionmentioning

confidence: 99%

Ginsenoside Rb1 alleviates airway inflammation in asthma by regulating mitochondrial dysfunction through SIRT1/PGC-1α and PI3K/AKT signaling pathway

Li,

Piao,

Bai

et al. 2024

Preprint

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Aim of this study is to investigate whether Ginsenoside Rb1 attenuates cockroach extract (CRE) induced asthma by interfering with mitochondrial dysfunction. After induction of CRE, mice were administrated different dose of Rb1. HE staining, ELISA and flow cytometry analysis showed that, the inflammatory cell infiltration, total IgE and CRE specific IgE in serum, and inflammatory cytokines in bronchoalveolar lavage fluid (BALF) were effectively inhibited by Rb1. Through Western blot, TUNEL and immunofluorescence co-localization assay, we observed Rb1 also inhibited endogenous reactive oxygen species (ROS), tightly associated with increased superoxide dismutase (SOD), catalase (CAT) levels, and decreased malondialdehyde (MDA). Subsequently, the silent information regulator Sirtuni1(SIRT1)/peroxisome proliferator-activated receptor-γ coactivator α (PGC-1α) pathway were activated, whereas, phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT) signaling pathway were alleviated. All of which led to mitochondria dysfunction via promoting mitochondrial fusion protein Mitofusion 1 (MFN1) and inhibiting dynamin-related protein 1 (DRP1) expression and apoptosis in lungs. In BEAS-2B cells, Rb1 played a similar role as SIRT1 agonist (SRT1720), including mitochondrial membrane potential enhancement, mitochondrial ROS and DRP1 translocation to mitochondria decrease. Our findings suggest that Rb1 maintains mitochondria integrity by activating SIRT1/PGC-1α, inhibiting PI3K/AKT, thereby ameliorates asthmatic airway inflammation.

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“…In Table 2 , recent literatures published within the past three years that can help to understand the molecular mechanisms through which ginseng acts in brain plasticity are summarized. Ginseng has influenced brain plasticity by affecting various cells within the central nervous system, including neurons [ 41 , 56 , [73] , [74] , [75] , [76] , [77] , [78] ], oligodendrocytes [ [79] , [80] , [81] , [82] ], astrocytes [ 83 , 84 ], microglia [ 82 , 85 ], and peripheral systems such as gut microbiota [ 69 , 70 , 86 ]. In terms of molecular level, the effects of ginseng have been observed in the altered levels of neurotransmitters (such as serotonin [ [73] , [74] , [75] , 86 ], dopamine [ 74 , 86 ], and gamma-aminobutyric acid (GABA) [ 73 ]), N-methyl- d -aspartate receptor (NMDAR) subunits [ 76 ], synaptic proteins [ 56 ], and Brain-Derived Neurotrophic Factor (BDNF) [ 56 , 73 , 74 ].…”

Section: Ginseng's Molecular Mechanisms In Brain Plasticitymentioning

confidence: 99%

“…In terms of molecular level, the effects of ginseng have been observed in the altered levels of neurotransmitters (such as serotonin [ [73] , [74] , [75] , 86 ], dopamine [ 74 , 86 ], and gamma-aminobutyric acid (GABA) [ 73 ]), N-methyl- d -aspartate receptor (NMDAR) subunits [ 76 ], synaptic proteins [ 56 ], and Brain-Derived Neurotrophic Factor (BDNF) [ 56 , 73 , 74 ]. It has induced anti-oxidation [ 41 , 74 ] and anti-inflammation [ 56 , 70 , 85 ], increased mitochondrial function and biogenesis [ 78 , 83 ], enhanced the functions of astrocyte gap junctions [ 84 ], and the BBB [ 69 ]. Moreover, ginseng was involved in cell proliferation and differentiation [ 79 , 80 , 83 , 84 ].…”

Section: Ginseng's Molecular Mechanisms In Brain Plasticitymentioning

confidence: 99%

Brain plasticity and ginseng

Shin,

Lee,

Cho

et al. 2024

Journal of Ginseng Research

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Major ginsenosides from Panax ginseng promote aerobic cellular respiration and SIRT1-mediated mitochondrial biosynthesis in cardiomyocytes and neurons

Cited by 10 publications

References 48 publications

Systematic Insight of Resveratrol Activated SIRT1 Interactome through Proximity Labeling Strategy

Systematic Insight of Resveratrol Activated SIRT1 Interactome through Proximity Labeling Strategy

Ginsenoside Rb1 alleviates airway inflammation in asthma by regulating mitochondrial dysfunction through SIRT1/PGC-1α and PI3K/AKT signaling pathway

Brain plasticity and ginseng

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