Major genes control hormone-induced ovulation rate in mice

Spearow, Jimmy L.

doi:10.1530/jrf.0.0820787

Cited by 41 publications

(46 citation statements)

References 22 publications

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“…PMSG at 28 days and 5 i.u. hCG at 30 days (Spearow, 1988 (Brown & Forsythe, 1974;Dixon, 1983 Means within columns with different superscripts differ significantly (P < 0-05). (P < 001).…”

Section: Introductionmentioning

confidence: 99%

“…Major genetic differences in hormone-induced ovulation rate in young mice have been identified (Spearow, 1988): a comparison of parentals, Fl and F2 crosses, revealed that a 5-fold difference in hormone-induced ovulation rate between strains A/J and SJL/J was due to the action of about 2-3 loci, and a 6-fold difference in hormone-induced ovulation rate between strains A/J and C57BL/6J was due to the action of about 3^1 loci. These genetic differences in hormone-induced ovulation rate at 28 days of age could have been due to genetic differences in (1) age at puberty, (2) maturation and ovulation of follicles by endogenous gonadotrophins, (3) maturation of follicles by endogenous gonadotrophins and ovulation by exogenous gonadotrophins, (4) age-dependent responses to gonadotrophins, or (5) ovarian responsiveness to exogenous PMSG and hCG.…”

Section: Introductionmentioning

confidence: 99%

“…At 16-22 h later the mice were weighed, and killed by cervical dislocation. The number of ova with cumulus (fresh ova) and without cumulus (old ova) in the oviduct was then determined according to the procedures of Spearow (1988). Since the fresh ova were recovered in a tight cumulus clump, their recovery was essentially complete.…”

Section: Introductionmentioning

confidence: 99%

“…PMSG at 28 days and 5 i.u. hCG at 30 days of age (Spearow, 1988 Experiment 5. Responses to increasing doses of oFSH and a constant amount of hCG were examined in strains A/J, SJL/J and C57BL/6J.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of genetic differences in hormone-induced ovulation rate in mice

Spearow

1988

Reproduction

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Summary. Major genetic differences in hormone-induced ovulation rate were not explained by strain differences in age at puberty, by maturation and ovulation of follicles by endogenous gonadotrophins, or by differential responses to gonadotrophins at different ages. The major genetic differences in hormone-induced ovulation rate were explained by strain differences in ovarian responsiveness to exogenous gonadotrophins.

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“…PMSG at 28 days and 5 i.u. hCG at 30 days (Spearow, 1988 (Brown & Forsythe, 1974;Dixon, 1983 Means within columns with different superscripts differ significantly (P < 0-05). (P < 001).…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Characterization of genetic differences in hormone-induced ovulation rate in mice

Spearow

1988

Reproduction

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“…The second factor, production of fertilized eggs, depends on biological factors that are not always under control of the investigator. For example, some inbred strains respond poorly to superovulation treatments (Spearow, 1988) and produce few eggs for fertilization. Other inbred strains, such as PL/J have poor sperm quality that affect numbers of fertilized eggs (Pyle & Handel, 2003;T.…”

Section: Discussionmentioning

confidence: 99%

Rederivation of Transgenic and Gene-Targeted Mice by Embryo Transfer

Keuren

Saunders

2004

Transgenic Res

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Research on genetically engineered mice provides insights into the etiology, therapy, and genetic basis of human diseases. An important variable that affects the results of mouse studies is the health status of the animals. Pathogen burdens may confound observations and obscure underlying mechanisms. Mouse resource centers frequently rederive infected mouse strains. We review our experience on the use of a well-established technique, embryo transfer to rederive infected mouse strains. The following mouse pathogens were eliminated by embryo transfer: Mouse Parvovirus, Mouse Hepatitis Virus, Mouse Rotavirus, Mouse Encephalomyelitis Virus, Mouse Adenovirus, Helicobacter species, endoparasites, and ectoparasites. We rederived transgenic mouse lines, gene-targeted mouse lines, and lines with spontaneous mutations. In the majority of strains, fertilized eggs for embryo transfer were obtained by mating superovulated egg donors with males of the desired genotype. A total of 309 embryo transfers were performed to rederive 96 mouse strains. The pregnancy rate was 76%; 1996 pups were born, of which 43% carried the desired genotype. We performed 44 additional embryo transfers to rederive 15 other strains. The pregnancy rate was lower (45%) and none of the 135 pups carried the desired genotype. Although we successfully eliminated the pathogens in all transfers, we were unable to obtain pups with the desired genotype in 15 of 111 mouse lines. Multiple factors affect the efficiency of rederivation by embryo transfer. They include the response to superovulation by embryo donors, the number and age of stud males, the yield of fertilized eggs, the number of embryo transfers, and genotyping.

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In vitro fertilization of gonadotropin‐stimulated leopard cat (Felis bengalensis) follicular Oocytes

1989

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Based on techniques developed for the domestic cat, in vitro fertilization (IVF) studies were conducted in the taxonomically related leopard cat (Felis bengalensis). Adult females received pregnant mares' serum gonadotropin (PMSG) followed 80 or 84 h later by human chorionic gonadotropin (hCG) on two to four occasions over a 40-day to 27-month interval. Oocytes were collected laparoscopically from ovarian follicles 25-27 h after hCG and co-cultured with processed, homologous spermatozoa (37 degrees C, 5% CO2 in air, humidified atmosphere) for 30-36 h. There was no apparent ovarian refractoriness to repeated treatments with exogenous gonadotropins. Overall, the mean number of mature follicles present and the total number of oocytes and proportion of immature oocytes collected did not differ (P greater than 0.05) between the 80 h (4.9 +/- 0.9; 4.7 +/- 1.2; 14.9%, respectively) and 84 h (5.6 +/- 1.4; 5.4 +/- 1.7; 22.2%, respectively) gonadotropin interval groups. However, the proportion of mature leopard cat oocytes fertilized in vitro, as determined by embryonic cleavage, was increased (P less than 0.005) by extending the interval between PMSG and hCG from 80 (17.5%) to 84 (52.4%) h. These data 1) demonstrate that, compared to the domestic cat, the ovaries of the leopard cat are less responsive to a given PMSG/hCG treatment; 2) indicate that leopard cat follicular oocytes can be recovered readily by laparoscopy and are capable of becoming fertilized in vitro; and 3) suggest that IVF may be a viable approach for producing embryos and perhaps enhancing captive propagation of rare Felidae.

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Major genes control hormone-induced ovulation rate in mice

Cited by 41 publications

References 22 publications

Characterization of genetic differences in hormone-induced ovulation rate in mice

Characterization of genetic differences in hormone-induced ovulation rate in mice

Rederivation of Transgenic and Gene-Targeted Mice by Embryo Transfer

In vitro fertilization of gonadotropin‐stimulated leopard cat (Felis bengalensis) follicular Oocytes

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