Major-effect QTLs for stem and foliage resistance to late blight in the wild potato relatives Solanum sparsipilum and S. spegazzinii are mapped to chromosome X

Danan, Sarah; Chauvin, Jean-Éric; Caromel, Bernard; Moal, Jean-Denis; Pellé, Roland; Lefebvre, Véronique

doi:10.1007/s00122-009-1081-7

Cited by 25 publications

(22 citation statements)

References 82 publications

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“…The observed QTL correspondence was found to be unlikely because of chance alone, according to a statistical test based on the hypergeometric probability function. A number of studies of the potato have reported a significant positive correlation of increased resistance to P. infestans with late maturity (Toxopeus 1958; Collins et al 1999; Bradshaw et al 2004; Danan et al 2009). Linkage of resistance and maturity QTL in potato was noted on chromosome 5, which is syntenic to tomato chromosome 5 (Collins et al 1999; Oberhagemann et al 1999; Visker et al 2003; Bradshaw et al 2004; Visker et al 2005).…”

Section: Discussionmentioning

confidence: 99%

Linkage Relationships Among Multiple QTL for Horticultural Traits and Late Blight (P. infestans) Resistance on Chromosome 5 Introgressed from Wild TomatoSolanum habrochaites

Haggard

Johnson

Clair

2013

G3 Genes|Genomes|Genetics

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When the allele of a wild species at a quantitative trait locus (QTL) conferring a desirable trait is introduced into cultivated species, undesirable effects on other traits may occur. These negative phenotypic effects may result from the presence of wild alleles at other closely linked loci that are transferred along with the desired QTL allele (i.e., linkage drag) and/or from pleiotropic effects of the desired allele. Previously, a QTL for resistance to Phytophthora infestans on chromosome 5 of Solanum habrochaites was mapped and introgressed into cultivated tomato (S. lycopersicum). Near-isogenic lines (NILs) were generated and used for fine-mapping of this resistance QTL, which revealed coincident or linked QTL with undesirable effects on yield, maturity, fruit size, and plant architecture traits. Subsequent higher-resolution mapping with chromosome 5 sub-NILs revealed the presence of multiple P. infestans resistance QTL within this 12.3 cM region. In our present study, these sub-NILs were also evaluated for 17 horticultural traits, including yield, maturity, fruit size and shape, fruit quality, and plant architecture traits in replicated field experiments over the course of two years. Each previously detected single horticultural trait QTL fractionated into two or more QTL. A total of 41 QTL were detected across all traits, with ∼30% exhibiting significant QTL × environment interactions. Colocation of QTL for multiple traits suggests either pleiotropy or tightly linked genes control these traits. The complex genetic architecture of horticultural and P. infestans resistance trait QTL within this S. habrochaites region of chromosome 5 presents challenges and opportunities for breeding efforts in cultivated tomato.

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Section: Discussionmentioning

confidence: 99%

Linkage Relationships Among Multiple QTL for Horticultural Traits and Late Blight (P. infestans) Resistance on Chromosome 5 Introgressed from Wild TomatoSolanum habrochaites

Haggard

Johnson

Clair

2013

G3 Genes|Genomes|Genetics

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“…Apart from R genes, a number of QTL for resistance to P. infestans have been mapped to potato chromosome X [25,34,47-49]. These QTL originate from various Solanum species, including S .…”

Section: Discussionmentioning

confidence: 99%

“…These QTL originate from various Solanum species, including S . sparsipilum , which is a synonym of S. ruiz-ceballosii [25]. A QTL meta-analysis that allowed more precise comparisons of different genetic maps has identified two meta-QTL for late blight resistance on potato chromosome X.…”

Section: Discussionmentioning

confidence: 99%

“…Quantitative trait locus for late blight resistance with major effects originating from the synonymous S. sparsipilum has previously been mapped on potato chromosome X using stem and foliage tests and explained up to 29% of variance in the stem resistance of the mapping population [25]. S. sparsipilum has been also described as a source of resistance to the nematodes Meloidogyne fallax [26] and Globodera pallida [27] as well as to Potato Virus Y (PVY) [28].…”

Section: Introductionmentioning

confidence: 99%

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Late blight resistance gene from Solanum ruiz-ceballosii is located on potato chromosome X and linked to violet flower colour

et al. 2012

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BackgroundPhytophthora infestans (Mont.) de Bary, the causal organism of late blight, is economically the most important pathogen of potato and resistance against it has been one of the primary goals of potato breeding. Some potentially durable, broad-spectrum resistance genes against this disease have been described recently. However, to obtain durable resistance in potato cultivars more genes are needed to be identified to realize strategies such as gene pyramiding or use of genotype mixtures based on diverse genes.ResultsA major resistance gene, Rpi-rzc1, against P. infestans originating from Solanum ruiz-ceballosii was mapped to potato chromosome X using Diversity Array Technology (DArT) and sequence-specific PCR markers. The gene provided high level of resistance in both detached leaflet and tuber slice tests. It was linked, at a distance of 3.4 cM, to violet flower colour most likely controlled by the previously described F locus. The marker-trait association with the closest marker, violet flower colour, explained 87.1% and 85.7% of variance, respectively, for mean detached leaflet and tuber slice resistance. A genetic linkage map that consisted of 1,603 DArT markers and 48 reference sequence-specific PCR markers of known chromosomal localization with a total map length of 1204.8 cM was constructed.ConclusionsThe Rpi-rzc1 gene described here can be used for breeding potatoes resistant to P. infestans and the breeding process can be expedited using the molecular markers and the phenotypic marker, violet flower colour, identified in this study. Knowledge of the chromosomal localization of Rpi-rzc1 can be useful for design of gene pyramids. The genetic linkage map constructed in this study contained 1,149 newly mapped DArT markers and will be a valuable resource for future mapping projects using this technology in the Solanum genus.

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“…Quantitative resistance is expected to be more durable than qualitative resistance since it is governed by multigenes and provides resistance to diverse pathogen races (Rauscher et al 2010). To date, more than 20 research articles have focused on quantitative trait locus (QTL) analysis of potato late blight resistance (Danan et al 2009;LeonardsSchippers et al 1994;Mihovilovich et al 2010;Rauscher et al 2010;Ritter et al 2009;Simko 2002). Each of these previously reported sets of QTL was detected on the basis of a phenotypic value that represents disease accumulated during the evaluation period.…”

Section: Introductionmentioning

confidence: 99%

Conditional QTL underlying resistance to late blight in a diploid potato population

Lindqvist‐Kreuze²,

Tian

et al. 2012

Theor Appl Genet

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A large number of quantitative trait loci (QTL) for resistance to late blight of potato have been reported with a "conventional" method in which each phenotypic trait reflects the cumulative genetic effects for the duration of the disease process. However, as genes controlling response to disease may have unique contributions with specific temporal features, it is important to consider the phenotype as dynamic. Here, using the net genetic effects evidenced at consecutive time points during disease development, we report the first conditional mapping of QTL underlying late blight resistance in potato under five environments in Peru. Six conditional QTL were mapped, one each on chromosome 2, 7 and 12 and three on chromosome 9. These QTL represent distinct contributions to the phenotypic variation at different stages of disease development. By comparison, when conventional mapping was conducted, only one QTL was detected on chromosome 9. This QTL was the same as one of the conditional QTL. The results imply that conditional QTL reflect genes that function at particular stages during the host-pathogen interaction. The dynamics revealed by conditional QTL mapping could contribute to the understanding of the molecular mechanism of late blight resistance and these QTL could be used to target genes for marker development or manipulation to improve resistance.

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Major-effect QTLs for stem and foliage resistance to late blight in the wild potato relatives Solanum sparsipilum and S. spegazzinii are mapped to chromosome X

Cited by 25 publications

References 82 publications

Linkage Relationships Among Multiple QTL for Horticultural Traits and Late Blight (P. infestans) Resistance on Chromosome 5 Introgressed from Wild TomatoSolanum habrochaites

Linkage Relationships Among Multiple QTL for Horticultural Traits and Late Blight (P. infestans) Resistance on Chromosome 5 Introgressed from Wild TomatoSolanum habrochaites

Late blight resistance gene from Solanum ruiz-ceballosii is located on potato chromosome X and linked to violet flower colour

Conditional QTL underlying resistance to late blight in a diploid potato population

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