Major effect loci for plant size before onset of nitrogen fixation allow accurate prediction of yield in white clover

Moeskjær, Sara; Skovbjerg, Cathrine Kiel; Tausen, Marni; Wind, Rune; Roulund, Niels; Janss, Luc; Andersen, Stig Uggerhøj

doi:10.1007/s00122-021-03955-3

Cited by 6 publications

(15 citation statements)

References 50 publications

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“…In a recent study performed with combinations of Rhizobium strains and genotypes from white clover cultivars, Moeskjaer et al (2021) reported low to moderate broad-sense heritabilities, on an individual plant phenotype level, ranging from 0.23 to 0.32 for a set of symbiotic traits including: growth per day; growth during a specific window post-inoculation; and corrections of these for initial plant size. When using a genomic relationship matrix to derive

based on clover genotype means, the values for these traits ranged from 0.24 to 0.27, when corrected for initial plant size ( Moeskjaer et al, 2021 ). This aligns with the symbiotic yield

values described in the current study.…”

Section: Discussionmentioning

confidence: 99%

“…This aligns with the symbiotic yield

values described in the current study. The trial reported by Moeskjaer et al (2021) assessed stolon cuttings, which represent the mature clover nodal-rooting growth stage, from a range of genetic material. By contrast, the current study focussed on seedlings from a half-sib family structure growing on their initial taproot.…”

Section: Discussionmentioning

confidence: 99%

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Quantitative genetic analysis reveals potential to breed for improved white clover growth in symbiosis with nitrogen-fixing Rhizobium bacteria

et al. 2022

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White clover (Trifolium repens) is integral to mixed pastures in New Zealand and temperate agriculture globally. It provides quality feed and a sustainable source of plant-available nitrogen (N) via N-fixation through symbiosis with soil-dwelling Rhizobium bacteria. Improvement of N-fixation in white clover is a route to enhancing sustainability of temperate pasture production. Focussing on seedling growth critical for crop establishment and performance, a population of 120 half-sibling white clover families was assessed with either N-supplementation or N-fixation via inoculation with a commercial Rhizobium strain (TA1). Quantitative genetic analysis identified significant (p < 0.05) family additive genetic variance for Shoot and Root Dry Matter (DM) and Symbiotic Potential (SP), and Root to Shoot ratio. Estimated narrow-sense heritabilities for above-ground symbiotic traits were moderate (0.24–0.33), and the strong (r ≥ 0.97) genetic correlation between Shoot and Root DM indicated strong pleiotropy or close linkage. The moderate (r = 0.47) phenotypic correlation between Shoot DM under symbiosis vs. under N-supplementation suggested plant growth with mineral-N was not a strong predictor of symbiotic performance. At 5% among-family selection pressure, predicted genetic gains per selection cycle of 19 and 17% for symbiotic traits Shoot DM and Shoot SP, respectively, highlighted opportunities for improved early seedling establishment and growth under symbiosis. Single and multi-trait selection methods, including a Smith-Hazel index focussing on an ideotype of high Shoot DM and Shoot SP, showed commonality of top-ranked families among traits. This study provides a platform for proof-of-concept crosses to breed for enhanced seedling growth under Rhizobium symbiosis and is informative for other legume crops.

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based on clover genotype means, the values for these traits ranged from 0.24 to 0.27, when corrected for initial plant size ( Moeskjaer et al, 2021 ). This aligns with the symbiotic yield

values described in the current study.…”

Section: Discussionmentioning

confidence: 99%

“…This aligns with the symbiotic yield

Section: Discussionmentioning

confidence: 99%

Quantitative genetic analysis reveals potential to breed for improved white clover growth in symbiosis with nitrogen-fixing Rhizobium bacteria

et al. 2022

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“…Plants were initially grown in the greenhouse as part of a larger greenhouse trial investigating the effects of clover and rhizobium genetic variation through monitoring of plant growth traits (Moeskjaer et al, 2022). Mother plants of 20 commercial varieties with known genotypes were initially grown and cuttings were taken.…”

Section: Greenhouse Trialmentioning

confidence: 99%

“…overall plant size, leaf markings, plant density, leaf colour, internode length and stolon density (Moeskjaer et al, 2022). The crosses used in the field trial were not used in the greenhouse trial due to the intellectual property rights of DLF Trifolium breeder trial.…”

Section: Greenhouse Trialmentioning

confidence: 99%

“…Plant yield and greenness were measured during the greenhouse experiment, as detailed in Moeskjaer et al (2022) and Tausen et al (2020) respectively. Briefly, the growth and greenness of plants throughout the greenhouse experiment were monitored using a greenhouse overhead camera imaging system (Moeskjaer et al, 2022;Tausen et al, 2020).…”

Section: Greenhouse Trialmentioning

confidence: 99%

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Rhizobium nodule diversity and composition are influenced by clover host selection and local growth conditions

et al. 2023

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Screening and field evaluation of white clover rhizobia for New Zealand pastures

Shusen

Wakelin

Gerard

et al. 2023

Crop & Pasture Science

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Context. Biological nitrogen (N) fixation through the rhizobia-legume symbiosis is a sustainable and cost-effective source of N for agriculture. In New Zealand (NZ), white clover (Trifolium repens) is a key component of pastures and rhizobial inoculation of clover is widely used. The current commercial inoculant for white clover, TA1, was isolated in Australia in the 1950s and may not be the best partner for modern white clover cultivars. Aims. To identify Rhizobium leguminosarum bv. trifolii (Rlt) isolates suitable for use in NZ pastures. Methods. The symbiotic potential of >230 isolates collected from throughout NZ was evaluated in plant bioassays. Selected isolates were further evaluated in pot and field trials. Key results. Approximately 40% of NZ isolates supported better clover growth than TA1 under N-limited conditions in vitro. Of 24 Rlt isolates evaluated in a glasshouse trial, five produced significantly higher clover biomass than TA1. Three (S11N9, S20N7, S4N6) of nine isolates evaluated in two field trials in 2018-2019 significantly increased clover growth (12-38%) compared with paired uninoculated clover at several harvests, whereas inoculation with TA1 did not improve yield. In a third trial in 2020, S11N9 and S20N7 increased clover growth compared with the uninoculated control at two of three harvests; S4N6 performed better than TA1 at one harvest. When tested with four white clover cultivars, five Rlt isolates had higher symbiotic potential than TA1. Conclusions. Inoculating white clover would be beneficial if improved inoculant isolates were available. Implications. We recommend some NZ Rlt isolates could be developed into commercial inoculants to improve white clover performance in NZ.

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Major effect loci for plant size before onset of nitrogen fixation allow accurate prediction of yield in white clover

Cited by 6 publications

References 50 publications

Quantitative genetic analysis reveals potential to breed for improved white clover growth in symbiosis with nitrogen-fixing Rhizobium bacteria

Quantitative genetic analysis reveals potential to breed for improved white clover growth in symbiosis with nitrogen-fixing Rhizobium bacteria

Rhizobium nodule diversity and composition are influenced by clover host selection and local growth conditions

Screening and field evaluation of white clover rhizobia for New Zealand pastures

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