Major Compounds from Ocimum Basilicum l. and their Antimicrobial Activity against Methicillin-Resistant Staphylococcus Aureus

Abstract

Sweet Basil (Ocimum basilicum Linn.) has a variety of therapeutic properties, including antimicrobial activity. This study aimed to determine the antibacterial activity of the major chemical components in the crude extract against methicillin resistant Staphylococcus aureus (MRSA) isolated from the clinical specimens. TLC and column chromatography resulted in the separation of two major compounds. On the basis of the spectral data, they were identified as linalool (1) and 1,8-cineole (2). Antibacterial activity of crude extract and isolated compounds was evalusted by disc diffusion, MIC and MBC against 9 multi-drug resistance S. aureus isolated from clinical specimens. The crude extract and isolated compounds exhibited an excellent antimicrobial activity at a dose level of 34.5 mg/disc for disc diffusion method. The crude extract and linalool showed higher activity against tested MRSA with MIC (<0.09 mg/ml) and MBC (≤ 0.09 to 0.38 mg/ml) than 1,8-cineole. The results suggest once again that the antimicrobial activity of the crude extract and major isolated compounds, linalool and 1,8-cineole are a resultant of the antibacterial property against MRSA.

Effects of Dietary Supplement of Basil Extract on Biochemical and Immunological Parameters and Growth Performance in Oncorhynchus mykiss

“…The antimicrobial properties of basil have been demonstrated in various studies [50,76]. Taecowisan et al [77] indicated that the basil's antibacterial effect is related to the major components of basil extract, namely linalool and 1,8-cineol. Many studies have also shown the stimulatory effects of various herbal medicines, including Lawsonia inermis [78], oregano herb [74], and Artemisia Drncuculus [30], in boosting the immune system.…”

Natural Compounds with Antimicrobial Properties in Cosmetics

“…As reported by Taechowisan et al, Ocimum basilicum Linn. crude extract is an active agent against methicillin-resistant Staphylococcus aureus (MRSA) [ 52 ]. Using the diffusion method, the researchers determined the very high microbial activity of the crude extract at 34.5 mg/disc.…”

“…Using the diffusion method, the researchers determined the very high microbial activity of the crude extract at 34.5 mg/disc. Moreover, they noticed that the crude extract and the main isolated compound linalool showed higher activity against MRSA with MIC < 0.09 mg/mL and MBC ≤ 0.09 to 0.38 mg/mL than 1,8-cineole [ 52 ]. Kaya et al conducted research on basil extracts obtained using various extractants: chloroform, acetone, and methanol (in two different water resuspension doses of 5 and 10 mL).…”

Bauhinia rufescens, Ocimum basilicum and Salvadora persica: a review of their chemical compounds and properties for antimicrobial, antioxidant and cytotoxicity

“…The recent research has shown that the essential oil of Ocimum basilicum has an inhibiting action on certain fungi such as Rhizoctonia, Macrophomina phaseolina, Aspergillus niger, Aspergillus fumigates, Aspergillus flavus, Candida albicans and Penicillium [37,38]. Thongchai et al carried out a thorough study on the leaf extracts of Ocimum basilicum [39]. They have been investigated against Methicillin-resistant Staphylococcus aureus (MRSA) isolated from clinical samples.…”