Maize tissue culture, transformation, and genome editing

Kausch, Albert P.; Nelson‐Vasilchik, Kimberly; Tilelli, Michael; Hague, Joel

doi:10.1007/s11627-021-10196-y

Cited by 11 publications

(15 citation statements)

References 176 publications

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“…The most commonly used maize genotype in academic laboratories is the single hybrid Hi-II ( Armstrong et al, 1991 ) and their inbred parents ( Frame et al, 2006 ; Ishida et al, 2007 ). However, although these genotypes show high-frequency Agrobacterium infection of IZEs, embryogenic callus formation, good performance in selective medium, and recovery of transformed plants, they lack the minimal agronomic performance needed for phenotyping characterization ( Frame et al, 2006 ; Wang et al, 2009 ; Kausch et al, 2021a ). In addition, events generated from individual embryos produced by self-pollinated Hi-II hybrid plants have different genetic backgrounds.…”

Section: Current Status Of Maize Transformationmentioning

confidence: 99%

“…Although attempts have been made to use different explants ( Mu et al, 2012 ), almost all current protocols use IZEs for Agrobacterium -mediated transformation due to the well-established callus induction and somatic embryogenesis obtained with this explant ( Wang et al, 2009 ; Yadava et al, 2017 ; Kausch et al, 2021b ). IZEs give rise to type II callus-induced somatic embryos that, upon efficient selection, produce regenerated transformed events ( Kausch et al, 2021a ).…”

Section: Current Status Of Maize Transformationmentioning

confidence: 99%

“…Tissue culture-independent methods for plant transformation in plants have been limited to a few species-tissue/cell type systems (for recent reviews, see Kausch et al, 2021a , 2021b and Anjanappa and Gruissem, 2021 ). One of these methods relies on delivering DNA constructs into mature pollen that is then used for direct pollination ( Eapen, 2011 ).…”

Section: Emerging Technologies For Maize Transformationmentioning

confidence: 99%

“…The progress in maize transformation protocols over the years aimed to overcome genotype recalcitrance and produce less complex and unfragmented transgene insertions in the plant genome, which is a major drawback associated with the regulatory aspects of transgenics ( Kausch et al, 2021a ). As a result, Agrobacterium -mediated transformation has become the most suitable protocol for selecting single and unfragmented insertion events.…”

Section: Introductionmentioning

confidence: 99%

“…As a critical technology, maize transformation has been the central theme of several reviews covering different aspects ranging from historical and current advances in transformation protocols, methods, and applications ( Wang et al, 2009 ; Que et al, 2014 ; Yadava et al, 2017 ; Ishida et al, 2020 ; Kausch et al, 2021a , 2021b ). Here, we present the latest advances in the protocols and technologies for maize transformation and expand the topic to the development of new genetically engineered maize varieties, regulatory issues, and the importance of delivering new commercial biotech maize varieties to the market.…”

Section: Introductionmentioning

confidence: 99%

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Maize Transformation: From Plant Material to the Release of Genetically Modified and Edited Varieties

et al. 2021

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Over the past decades, advances in plant biotechnology have allowed the development of genetically modified maize varieties that have significantly impacted agricultural management and improved the grain yield worldwide. To date, genetically modified varieties represent 30% of the world’s maize cultivated area and incorporate traits such as herbicide, insect and disease resistance, abiotic stress tolerance, high yield, and improved nutritional quality. Maize transformation, which is a prerequisite for genetically modified maize development, is no longer a major bottleneck. Protocols using morphogenic regulators have evolved significantly towards increasing transformation frequency and genotype independence. Emerging technologies using either stable or transient expression and tissue culture-independent methods, such as direct genome editing using RNA-guided endonuclease system as an in vivo desired-target mutator, simultaneous double haploid production and editing/haploid-inducer-mediated genome editing, and pollen transformation, are expected to lead significant progress in maize biotechnology. This review summarises the significant advances in maize transformation protocols, technologies, and applications and discusses the current status, including a pipeline for trait development and regulatory issues related to current and future genetically modified and genetically edited maize varieties.

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Section: Current Status Of Maize Transformationmentioning

confidence: 99%

Section: Current Status Of Maize Transformationmentioning

confidence: 99%

Section: Emerging Technologies For Maize Transformationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Maize Transformation: From Plant Material to the Release of Genetically Modified and Edited Varieties

et al. 2021

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Genetic, molecular and physiological crosstalk during drought tolerance in maize (Zea mays): pathways to resilient agriculture

Peer,

Bhat,

Lone

et al. 2024

Planta

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Development of efficient, cost-effective in vitro micropropagation technique for threatened ethnomedicinal plant Clerodendrum indicum (L.) O. Kuntze

Kundu,

Sahani,

Adhikary

et al. 2024

Plant Cell Tiss Organ Cult

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Clerodendrum indicum (L.) O. Kuntze (Verbenaceae) is a threatened ethnomedicinal plant with many bioactive secondary metabolites that could alleviate chronic diseases like cough, asthma, jaundice, leprosy, syphilitic rheumatism, and septic wounds. Their natural growth has been severely challenged due to habitat loss and massive exploitation for medical applications, leading to the threatened status. Therefore, an in vitro micropropagation technique has been trialled to be used for eco-restoration and metabolite exploitation. Micropropagation via direct and indirect shoot organogenesis had been established from the different explants of this plant. Murashige and Skoog (MS) media supplemented with variable concentrations of NAA, IAA, and BAP produced callus, organogenesis and whole plant. The study revealed that nodal explants resulted in more signi cant responses than others. The shoot and root regeneration through callus was observed in the MS media supplemented with 4.0 mg/L 6-BA and 0.5 mg/L NAA with an 84% response rate after two weeks of incubation and an average 5.6 number of shoots per callus. Only root and shoot regeneration was observed using half-strength MS media with 2.0 mg/L 6-BA and 1.0 mg/L NAA and 6.0 mg/L 6-BA and 0.5 mg/L NAA, respectively, after two weeks. The plantlets acclimatization had an average of 80% survival rate. These in-vitro regenerated plants by direct shoot organogenesis and through callus induction methods might aid in harvesting a bulk amount of secondary metabolites without destroying the native habitat. Thus, the methods would lead to environmental restoration sustainably.

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Maize tissue culture, transformation, and genome editing

Cited by 11 publications

References 176 publications

Maize Transformation: From Plant Material to the Release of Genetically Modified and Edited Varieties

Maize Transformation: From Plant Material to the Release of Genetically Modified and Edited Varieties

Genetic, molecular and physiological crosstalk during drought tolerance in maize (Zea mays): pathways to resilient agriculture

Development of efficient, cost-effective in vitro micropropagation technique for threatened ethnomedicinal plant Clerodendrum indicum (L.) O. Kuntze

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