Maize Root Plasma Membranes Isolated by Aqueous Polymer Two-phase Partitioning: Assessment of Residual Tonoplast ATPase and Pyrophosphatase Activities

Faraday, Christopher D.; Spanswick, Roger M.

doi:10.1093/jxb/43.12.1583

Cited by 35 publications

(15 citation statements)

References 43 publications

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“…This evidence was obtained by electrophoretic and western blot analysis following solubilization of rice root plasma membrane fractions of high purity. The plasma membrane fraction obtained from rice roots for this study was comparable in markers for purity and contamination (Table I) to fractions previously obtained by phase partitioning from rice [ 15,161 and other plant root material [9].…”

Section: Discussionsupporting

confidence: 57%

“…IR36) were grown in hydroponic culture [9]. Roots (80 g) were harvested 11 days after imbibition and chopped m homogenization buffer (250 ml) by a food processor.…”

Section: Membrane Purification and Characterizationmentioning

confidence: 99%

“…The supematants were filtered through nylon mesh screens (149 pm openings) and a microsomal fraction was obtained by centrifugation at 85 000 x g for 35 min. A U,U,' plasma membrane fraction was obtained from the microsomal fraction by aqueous polymer two-phase partitioning [8] as presented in detail by [9]. The U,U( fraction was diluted four-fold with dilution/resuspension buffer (10 mM potassium phosphate, pH 8.0, 300 mM sorbitol, 10% (v/v) glycerol, 0.1 mM Na,EDTA, 0.8 mM PMSF) and centrifuged at 150 000 x g for 40 min.…”

Section: Membrane Purification and Characterizationmentioning

confidence: 99%

“…Protein was assayed [lo] with BSA as a standard. Membrane preparations were diluted into phosphate-free 300 mM sorbitol and 10% (v/v) glycerol before ahquots were taken for ATPase assays, which were conducted as in [9].…”

Section: Membrane Purification and Characterizationmentioning

confidence: 99%

“…The right-side-out orientation may 313 protect and preserve electrostatic interactions important to the integrity of protein complexes and peripheral protein associations with the membrane [9]. In the present study, aqueous polymer two-phase partitioning was used to obtain a purified plasma membrane fraction from rice roots.…”

Section: Introductionmentioning

confidence: 99%

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Evidence for a membrane skeleton in higher plants

Faraday

Spanswick

1993

FEBS Letters

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A fraction enriched in plasma membranes was isolated from rice roots by differential centrifugation and aqueous polymer two-phase partitioning.Analysis of the fraction by SDS-PAGE showed the presence of several low mobility polypeptides (M,> 100 kDa). One of these polypeptides (M, -230 kDa) was specifically recognized by polyclonal antibodies to human erythrocyte spectrin. This finding suggests that a higher plant spectrin-based membrane skeleton may be preserved and studied using high-purity plasma membrane fractions obtained by aqueous polymer two-phase partitioning.Aqueous polymer two-phase partitioning; Fodrm; Peripheral protein; Cytoskeleton-plasma membrane interaction

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Section: Discussionsupporting

confidence: 57%

“…IR36) were grown in hydroponic culture [9]. Roots (80 g) were harvested 11 days after imbibition and chopped m homogenization buffer (250 ml) by a food processor.…”

Section: Membrane Purification and Characterizationmentioning

confidence: 99%

Section: Membrane Purification and Characterizationmentioning

confidence: 99%

Section: Membrane Purification and Characterizationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Evidence for a membrane skeleton in higher plants

Faraday

Spanswick

1993

FEBS Letters

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Nitrate and ammonium nutrition of plants: Effects on acid/base balance and adaptation of root cell plasmalemma H⁺ATPase

Schubert

Yan

1997

J Plant Nutrition & Soil

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The increase of rhizosphere pH in the course of nitrate nutrition results from H+ consumption in the external medium during uptake of NO3− in a H+ co‐transport and from internal OH− production during nitrate reduction. Synthesis of organic acids for NH4+ assimilation as well as strong partial depolarization of membrane potential with NH4+ uptake are the important reasons for rhizosphere acidification during ammonium nutrition. Despite differences in proton balance depending on N form, cytoplasmic pH changes are small due to physico‐chemical buffering, biochemical pH regulation, H+ inclusion in vacuoles, and H+ release into the rhizosphere. Because of the large capacity for proton excretion the plasmalemma H+ ATPase of root cells plays an essential role during ammonium nutrition. An increase of the kinetic parameter Vmax after ammonium nutrition relative to nitrate nutrition suggests that the capacity of H+ release may be adjusted to the particular requirements of ammonium nutrition. Moreover, H+ ATPase is adjusted not only quantitatively but also qualitatively. The increase of the kinetic parameter km as well as the capability of the plasmalemma vesicles in vitro to establish a steeper pH gradient favours the supposition that H+ ATPase isoforms are formed which allow H+ release into the rhizosphere under conditions of low pH or poor H+ buffering of the soil. In this respect species differences exist, e.g. between maize (efficient adaptation) and faba bean (poor adaptation).

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Does H⁺ pumping by plasmalemma ATPase limit leaf growth of maize (Zea mays) during the first phase of salt stress?

Zörb

Stracke

Tramnitz

et al. 2005

Z. Pflanzenernähr. Bodenk.

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In the first phase of salt stress, growth of plants is impaired mainly by osmotic stress. To elucidate the effect of NaCl salinity on elongation growth of maize leaves in the first phase of salt stress, we investigated the effect of NaCl on gene expression and activity of the plasmalemma H+ ATPase of elongating leaves of maize (Zea mays L.). Treatment of maize plants with 125 mM NaCl for 3 d decreased leaf growth relative to control plants (1 mM NaCl). Whereas H+ ATPase hydrolytic activity was unaffected, the ability of the H+ ATPase to establish a pH gradient was strongly reduced. Total mRNA of plasmalemma H+ ATPase was slightly increased. However, mRNA of the ATPase isoform MHA1 was significantly reduced and ATPase isoform MHA4 was strongly increased at the mRNA level. Synthesis of total H+ ATPase protein was unchanged as revealed by western blot. The results indicate that reduced pumping of H+ ATPase in leaf plasmalemma under salt stress may be caused by a switch to gene expression of the specific isoform MHA4, which shows inferior H+‐pumping efficiency in comparison to isoforms expressed under control conditions. We propose that reduced H+ pumping of plasmalemma H+ ATPase is involved in the reduction of leaf growth of maize during the first phase of salt stress.

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Maize Root Plasma Membranes Isolated by Aqueous Polymer Two-phase Partitioning: Assessment of Residual Tonoplast ATPase and Pyrophosphatase Activities

Cited by 35 publications

References 43 publications

Evidence for a membrane skeleton in higher plants

Evidence for a membrane skeleton in higher plants

Nitrate and ammonium nutrition of plants: Effects on acid/base balance and adaptation of root cell plasmalemma H⁺ATPase

Does H⁺ pumping by plasmalemma ATPase limit leaf growth of maize (Zea mays) during the first phase of salt stress?

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Maize Root Plasma Membranes Isolated by Aqueous Polymer Two-phase Partitioning: Assessment of Residual Tonoplast ATPase and Pyrophosphatase Activities

Cited by 35 publications

References 43 publications

Evidence for a membrane skeleton in higher plants

Evidence for a membrane skeleton in higher plants

Nitrate and ammonium nutrition of plants: Effects on acid/base balance and adaptation of root cell plasmalemma H+ATPase

Does H+ pumping by plasmalemma ATPase limit leaf growth of maize (Zea mays) during the first phase of salt stress?

Contact Info

Product

Resources

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Nitrate and ammonium nutrition of plants: Effects on acid/base balance and adaptation of root cell plasmalemma H⁺ATPase

Does H⁺ pumping by plasmalemma ATPase limit leaf growth of maize (Zea mays) during the first phase of salt stress?