Maize rayado fino virus capsid proteins assemble into virus-like particles in Escherichia coli

Hammond, Rosemarie W.

doi:10.1016/j.virusres.2009.11.002

Cited by 15 publications

(8 citation statements)

References 28 publications

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“…This, however, requires further experimental confirmation. As previously suggested for MRFV, the ability of transiently expressed AMV CP25 to form VLPs may have potential value for applications in epitope presentation platforms [19]. …”

Section: Discussionmentioning

confidence: 99%

Characterization of alfalfa virus F, a new member of the genus Marafivirus

et al. 2018

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Viral infections of alfalfa are widespread in major cultivation areas and their impact on alfalfa production may be underestimated. A new viral species, provisionally named alfalfa virus F (AVF), was identified using a virion-associated nucleic acid (VANA) metagenomics-based approach in alfalfa (Medicago sativa L.) samples collected in Southern France. The nucleotide sequence of the viral genome was determined by de-novo assembly of VANA reads and by 5’/3’ RACE with viral RNA extracted from enriched viral particles or with total RNA, respectively. The virus shares the greatest degree of overall sequence identity (~78%) with Medicago sativa marafivirus 1 (MsMV1) recently deduced from alfalfa transcriptomic data. The tentative nucleotide sequence of the AVF coat protein shares ~83% identity with the corresponding region of MsMV1. A sequence search of the predicted single large ORF encoding a polyprotein of 235kDa in the Pfam database resulted in identification of five domains, characteristic of the genus Marafivirus, family Tymoviridae. The AVF genome also contains a conserved “marafibox”, a 16-nt consensus sequence present in all known marafiviruses. Phylogenetic analysis of the complete nucleotide sequences of AVF and other viruses of the family Tymoviridae grouped AVF in the same cluster with MsMV1. In addition to 5’ and 3’ terminal extensions, the identity of the virus was confirmed by RT-PCRs with primers derived from VANA-contigs, transmission electron microscopy with virus-infected tissues and transient expression of the viral coat protein gene using a heterologous virus-based vector. Based on the criteria demarcating species in the genus Marafivirus that include overall sequence identity less than 80% and coat protein identity less than 90%, we propose that AVF represents a distinct viral species in the genus Marafivirus, family Tymoviridae.

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Section: Discussionmentioning

confidence: 99%

Characterization of alfalfa virus F, a new member of the genus Marafivirus

et al. 2018

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“…After the cloning and expression in E. coli of the avibirnavirus IBDV VP2-, VP4-, and VP3-containing polyprotein gene (Table 3), the polyprotein was cotranslationally autoprocessed by protease VP4 activity, which enables the formation of immunologically active VLPs in bacterial cells [29]. However, as shown with the plant virus MRFV, the inclusion of the corresponding protease gene in the [30].…”

Section: Bacterial Systemsmentioning

confidence: 99%

“…Numerous studies demonstrate the ability of recombinant VLPs to bind and encapsidate nucleic acids that are present in the host cells, such as the mRNA of the transgene, ribosomal and/or transport RNAs [88,[107][108][109]. In some cases, the mRNA of the cloned gene is not found in the VLPs [30]. DNA microarray experiments suggest that CCMV VLPs, along with its own mRNA, are capable of encapsidating a large number of Pseudomonas mRNAs, except for the ribosomal RNA, which is the most abundant cell RNA [36].…”

Section: Characterization Of Vlpsmentioning

confidence: 99%

Construction and Characterization of Virus-Like Particles: A Review

2012

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Over the last three decades, virus-like particles (VLPs) have evolved to become a widely accepted technology, especially in the field of vaccinology. In fact, some VLP-based vaccines are currently used as commercial medical products, and other VLP-based products are at different stages of clinical study. Several remarkable advantages have been achieved in the development of VLPs as gene therapy tools and new nanomaterials. The analysis of published data reveals that at least 110 VLPs have been constructed from viruses belonging to 35 different families. This review therefore discusses the main principles in the cloning of viral structural genes, the relevant host systems and the purification procedures that have been developed. In addition, the methods that are used to characterize the structural integrity, stability, and components, including the encapsidated nucleic acids, of newly synthesized VLPs are analyzed. Moreover, some of the modifications that are required to construct VLP-based carriers of viral origin with defined properties are discussed, and examples are provided.

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“…3A). Hammond and Hammond (17) proposed that the two proteins may result from initiation at two independent initiation codons, although proteolytic processing to release the polymerase while forming a larger capsid protein as proposed for OBDV (10) cannot be ruled out.…”

Section: Infectivity In Plants and Protoplastsmentioning

confidence: 99%

Infectious Maize rayado fino virus from Cloned cDNA

et al. 2015

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A full-length cDNA clone was produced from a U.S. isolate of Maize rayado fino virus (MRFV), the type member of the genus Marafivirus within the family Tymoviridae. Infectivity of transcripts derived from cDNA clones was demonstrated by infection of maize plants and protoplasts, as well as by transmission via the known leafhopper vectors Dalbulus maidis and Graminella nigrifrons that transmit the virus in a persistent-propagative manner. Infection of maize plants through vascular puncture inoculation of seed with transcript RNA resulted in the induction of fine stipple stripe symptoms typical of those produced by wild-type MRFV and a frequency of infection comparable with that of the wild type. Northern and Western blotting confirmed the production of MRFV-specific RNAs and proteins in infected plants and protoplasts. An unanticipated increase in subgenomic RNA synthesis over levels in infected plants was observed in protoplasts infected with either wild-type or cloned virus. A conserved cleavage site motif previously demonstrated to function in both Oat blue dwarf virus capsid protein and tymoviral nonstructural protein processing was identified near the amino terminus of the MRFV replicase polyprotein, suggesting that cleavage at this site also may occur.

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Maize rayado fino virus capsid proteins assemble into virus-like particles in Escherichia coli

Cited by 15 publications

References 28 publications

Characterization of alfalfa virus F, a new member of the genus Marafivirus

Characterization of alfalfa virus F, a new member of the genus Marafivirus

Construction and Characterization of Virus-Like Particles: A Review

Infectious Maize rayado fino virus from Cloned cDNA

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