“…However, given that the 70S and 80S ribosomes of C. reinhardtii sedimented closely on sucrose gradients (Yamaguchi et al, 2003) and that mitochondrial ribosomes from higher plants have been observed to sediment anywhere between 70S (Vasconcelos and Bogorad, 1971; Pinel et al, 1986) and 78S (Leaver and Harmey, 1973, 1976; Pring, 1974), the above observations highlight the importance of early fractionation steps, orthogonal to sucrose gradient purification, in obtaining pure cytosolic ribosomes required for confident discrimination of cytosolic and organellar ribosomal proteomes. In this author’s view, this technical point is worth highlighting given the potential functional and evolutionary significance of parallel-targeting of r-proteins to multiple ribosomes in eukaryotic cells and the fact that just a few simple protocol modifications could greatly enhance the utility of future studies in addressing this important possibility.…”