Maize <i>reas1</i> Mutant Stimulates Ribosome Use Efficiency and Triggers Distinct Transcriptional and Translational Responses

Qi, Weiwei; Zhu, Jie; Wu, Qiao; Wang, Qun; Xia, Li; Yao, Dongsheng; Jin, Ying; Wang, Gang; Wang, Guifeng; Song, Rentao

doi:10.1104/pp.15.01722

Cited by 37 publications

(42 citation statements)

References 79 publications

(101 reference statements)

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“…1). The maize mutant dek* shows similar phenotypes, reflecting a delay in plant development according to decreased ZmRea1 activity15. However, the phenotype of dsr1 does not merely reflect delayed development, as the roots of 3-week-old plants were also severely short (Fig.…”

Section: Discussionmentioning

confidence: 88%

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The Mutation of Glu at Amino Acid 3838 of AtMDN1 Provokes Pleiotropic Developmental Phenotypes in Arabidopsis

et al. 2016

Sci Rep

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MDN1/Rea1, as an AAA-type ATPase, is predicted to be the largest protein involved in pre-ribosome maturation in most organisms. However, its function in plant growth and development is poorly understood. Here, we characterized a novel Arabidopsis mutant, dwarf & short root (dsr) 1, which shows pleiotropic developmental phenotypes, such as slow germination, short root, dwarf shoot, and reduced seed set under normal growth conditions. Using positional cloning, we revealed that the AtMDN1 function is impaired by a ‘glutamic acid’ to ‘lysine’ change at position 3838 of the amino acid sequence in dsr1. Multiple sequence alignment analysis revealed that the mutated Glu residue, which located in the linker domain of AtMDN1, is extremely conserved among organisms. AtMDN1 is expressed in various tissues, particularly in the shoot apex and root tip. Moreover, the results of transcript profile analyses showed that the dysfunction of AtMDN1 in dsr1 impairs the expression of genes related to plant growth and development, which is tightly associated with the pleiotropic phenotypes of dsr1. Thus, we concluded that the Glu residue plays a vital role in maintaining AtMDN1 functions, which are essential for plant growth and development.

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Section: Discussionmentioning

confidence: 88%

“…Recently, Qi et al . reported that a maize mutant with decreased Rea1 function, dek *, produces small kernels15. Plant growth and development is severely delayed as a result of impaired ribosome biogenesis in dek *15.…”

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confidence: 99%

The Mutation of Glu at Amino Acid 3838 of AtMDN1 Provokes Pleiotropic Developmental Phenotypes in Arabidopsis

et al. 2016

Sci Rep

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“…Interplay between kinases and phosphatases mediates global regulation of cellular function (Li et al, ; Trotta, Konert, Rahikainen, Aro, & Kangasjärvi, ; Zhao et al, ). The reversibility of protein phosphorylation status further aids in plasticity of molecular regulation spreading its influence over replication, transcription, cell cycle progression, splicing, and epigenetic regulation (Hur et al, ; Qi et al, ; Sun et al, ; Tunc‐Ozdemir et al, ). The precise site of phosphorylation has been the study of interest in deciphering signal transduction pathways (Nühse, Stensballe, Jensen, & Peck, ).…”

Section: Introductionmentioning

confidence: 99%

Dehydration‐responsive nuclear proteome landscape of chickpea (Cicer arietinum L.) reveals phosphorylation‐mediated regulation of stress response

Barua

Lande

Subba

et al. 2018

Plant Cell & Environment

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Nonavailability of water or dehydration remains recurring climatic disorder affecting yield of major food crops, legumes in particular. Nuclear proteins (NPs) and phosphoproteins (NPPs) execute crucial cellular functions that form the regulatory hub for coordinated stress response. Phosphoproteins hold enormous influence over cellular signalling. Four-week-old seedlings of a grain legume, chickpea, were subjected to gradual dehydration, and NPs were extracted from unstressed control and from 72- and 144-hr stressed tissues. We identified 4,832 NPs and 478 phosphosites, corresponding to 299 unique NPPs involved in multivariate cellular processes including protein modification and gene expression regulation, among others. The identified proteins included several novel kinases, phosphatases, and transcription factors, besides 660 uncharacterized proteins. Spliceosome complex and splicing related proteins were dominant among differentially regulated NPPs, indicating their dehydration modulated regulation. Phospho-motif analysis revealed stress-induced enrichment of proline-directed serine phosphorylation. Association mapping of NPPs revealed predominance of differential phosphorylation of spliceosome and splicing associated proteins. Also, regulatory proteins of key processes viz., protein degradation, regulation of flowering time, and circadian clock were observed to undergo dehydration-induced dephosphorylation. The characterization of novel regulatory proteins would provide new insights into stress adaptation and enable directed genetic manipulations for developing climate-resilient crops.

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“…Immunoblot analyses were performed as 737 described previously (Wang et al, 2014) using antibodies against the following nuclear proteins of 15 DAP maize immature kernels was carried out using a 754 previously described method (Qi et al, 2016b). …”

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confidence: 99%

OPAQUE11 Is a Central Hub of the Regulatory Network for Maize Endosperm Development and Nutrient Metabolism

et al. 2018

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Maize reas1 Mutant Stimulates Ribosome Use Efficiency and Triggers Distinct Transcriptional and Translational Responses

Cited by 37 publications

References 79 publications

The Mutation of Glu at Amino Acid 3838 of AtMDN1 Provokes Pleiotropic Developmental Phenotypes in Arabidopsis

The Mutation of Glu at Amino Acid 3838 of AtMDN1 Provokes Pleiotropic Developmental Phenotypes in Arabidopsis

Dehydration‐responsive nuclear proteome landscape of chickpea (Cicer arietinum L.) reveals phosphorylation‐mediated regulation of stress response

OPAQUE11 Is a Central Hub of the Regulatory Network for Maize Endosperm Development and Nutrient Metabolism

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