Maize fine streak virus, a New Leafhopper-Transmitted Rhabdovirus

Abstract

A previously uncharacterized virus was isolated from fall-planted sweet corn (Zea mays L., Syngenta GSS 0966) leaves showing fine chlorotic streaks. Symptomatic plants were negative in enzyme-linked immunosorbent assay against many maize viruses, but reacted weakly with antisera to Sorghum stunt mosaic virus suggesting a distant relationship between the viruses. The virus was readily transmitted by vascular puncture inoculation (VPI), but not by leaf-rub inoculation. Symptoms on maize included dwarfing and fine chlorotic streaks along intermediate and small veins that developed 12 to 17 days post-VPI. The isolated virus was bacilliform (231 +/- 5 nm long and 71 +/- 2 nm wide), with a knobby surface, and obvious helical structure typical of rhabdovirus morphology. Nucleorhabdovirus virions were observed by transmission electron microscopy of infected maize leaf tissue sections. Proteins unique to infected plants were observed in extracts of infected leaves, and the isolated virion contained three proteins with molecular masses 82 +/- 2, 50 +/- 3, and 32 +/- 2 kDa. Preliminary sequence analysis indicated the virus had similarity to members of the family Rhabdoviridae. The virus was transmitted by Graminella nigrifrons under persistent conditions. The data indicate the virus, provisionally designated Maize fine streak virus, is a new species in the genus Nucleorhabdovirus.

Large-scale recoding of an arbovirus genome to rebalance its insect versus mammalian preference

“…An example is Maize fine streak virus (MFSV), a nucleorhabdovirus that infects leafhoppers (an insect) and is transmitted to Zea mays (corn, a plant) and other plants (27,28). Again, the codon pairs present in the MFSV genome were strongly biased toward those pairs having high codon pair scores in both leafhoppers and corn ( Fig.…”

Complete Genome Sequence and In Planta Subcellular Localization of Maize Fine Streak Virus Proteins

“…Maize fine streak virus (MFSV) was first reported in maize fields in southwestern Georgia in 1999 and was described as a new plant nucleorhabdovirus (34). The symptoms caused by MFSV include dwarfing and fine chlorotic streaks along intermediate and small veins (34).…”

“…The symptoms caused by MFSV include dwarfing and fine chlorotic streaks along intermediate and small veins (34). MFSV is transmitted by the leafhopper Graminella nigrifrons and is not transmissible by rub inoculation of maize leaves but can be mechanically transmitted by vascular puncture inoculation (VPI) (20,34).…”

“…The symptoms caused by MFSV include dwarfing and fine chlorotic streaks along intermediate and small veins (34). MFSV is transmitted by the leafhopper Graminella nigrifrons and is not transmissible by rub inoculation of maize leaves but can be mechanically transmitted by vascular puncture inoculation (VPI) (20,34). Like those of other rhabdoviruses, the MFSV virion is a bacilliform particle measuring 231 by 71 nm with a lipid envelope, and its genome consists of a nonsegmented, negative-sense, singlestranded RNA (34,40).…”

Identificación y caracterización biológica del Barley yellow striate mosaic virus (BYSMV): nueva enfermedad del trigo en Argentina

“…Todos ellos son transmitidos por insectos, principalmente áfidos, cicadélidos y delfácidos (Hemiptera: Sternorryncha y Auchenorryncha). La transmisión por insectos es altamente específica dado que una especie de virus es transmitida por una o pocas especies relacionadas de insectos (Falk & Tsai, 1985;Redinbaugh et al, 2002). Por otra parte los insectos no sólo actúan como vectores sino que también son hospedantes para la replicación viral, constituyendo así una importante fuente de reservorio del virus.…”