Maize Doubled Haploids

Liu, Zhixin; Wang, Yan Bo; Ren, Jiaojiao; Mei, Mei; Frei, Ursula; Trampe, Benjamin; Lübberstedt, Thomas

doi:10.1002/9781119279723.ch3

Cited by 33 publications

(42 citation statements)

References 76 publications

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“…Briefly, GEM accessions were crossed with PHB47 and PHZ51 to produce F 1 seed, and most of these were grown and backcrossed once to PHB47 or PHZ51, respectively, to produce the BC 1 F 1 generation. BC 1 F 1 or F 1 plants were crossed with the inducer hybrid RWS 9 x RWK-76 [17] to produce haploid seed, which was identified based on the R-nj color marker [18]. In the subsequent planting season, putative haploids were grown in the greenhouse, and seedlings at the 3-4 leaf developmental stage were subjected to colchicine treatment to promote genome doubling.…”

Section: Plant Materialsmentioning

confidence: 99%

Genome-wide association studies of doubled haploid exotic introgression lines for root system architecture traits in maize (Zea mays L.)

et al. 2018

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Root system architecture (RSA) is becoming recognized as important for water and nutrient acquisition in plants. This study focuses on finding single nucleotide polymorphisms (SNPs) associated with seedling RSA traits from 300 doubled haploid (DH) lines derived from crosses between Germplasm Enhancement of Maize (GEM) accessions and inbred lines PHB47 and PHZ51. These DH lines were genotyped using 62,077 SNP markers, while root and shoot phenotype data were collected from 14-day old seedlings. Genome-wide association studies (GWAS) were conducted using three models to offset false positives/negatives. Multiple SNPs associated with seedling root traits were detected, some of which were within or linked to gene models that showed expression in seedling roots. Significant trait associations involving the SNP S5_152926936 on Chromosome 5 were detected in all three models, particularly the trait network area. The SNP is within the gene model GRMZM2G021110, which is expressed in roots at seedling stage. SNPs that were significantly associated with seedling root traits, and closely linked to gene models that encode proteins associated with root development were also detected. This study shows that the GEM-DH panel may be a source of allelic diversity for genes controlling seedling root development. KeywordsMaize, Zea mays L., Root system architecture, Germplasm enhancement of maize (GEM), GWAS, Doubled haploids RightsWorks produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted. A R T I C L E I N F O Keywords:Maize Zea mays L. Root system architecture Germplasm enhancement of maize (GEM) GWAS Doubled haploids A B S T R A C TRoot system architecture (RSA) is becoming recognized as important for water and nutrient acquisition in plants. This study focuses on finding single nucleotide polymorphisms (SNPs) associated with seedling RSA traits from 300 doubled haploid (DH) lines derived from crosses between Germplasm Enhancement of Maize (GEM) accessions and inbred lines PHB47 and PHZ51. These DH lines were genotyped using 62,077 SNP markers, while root and shoot phenotype data were collected from 14-day old seedlings. Genome-wide association studies (GWAS) were conducted using three models to offset false positives/negatives. Multiple SNPs associated with seedling root traits were detected, some of which were within or linked to gene models that showed expression in seedling roots. Significant trait associations involving the SNP S5_152926936 on Chromosome 5 were detected in all three models, particularly the trait network area. The SNP is within the gene model GRMZM2G021110, which is expressed in roots at seedling stage. SNPs that were significantly associated with seedling root traits, and closely linked to gene models that encode proteins associated with root development were also detected. This study shows that the GEM-DH panel may be a source of allelic diversity for genes controlling seedling root develo...

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Section: Plant Materialsmentioning

confidence: 99%

Genome-wide association studies of doubled haploid exotic introgression lines for root system architecture traits in maize (Zea mays L.)

et al. 2018

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“…Although alternatives exist, however, using an in-vivo maternal haploid inducer is typically the standard method in maize because it has been the most successful (Liu et al, 2016). One alternative is using an in-vivo paternal haploid inducer, which produces 1-2% haploid progeny due to the mutation of the gene ig1 (indeterminate gametophyte) (Kermicle, 1969;1971;.…”

Section: Commentary Background Informationmentioning

confidence: 99%

“…DH technology does not only save time and resources, but has many other benefits. For instance, this is the only method that will arrive at a completely homozygous and homogeneous line, and fulfills all requirements necessary for Plant Variety Protection (PVP) (Liu et al, 2016). In addition, selection during the haploid stage is more efficient and precise, especially when used in addition with molecular markers (Liu et al, 2016).…”

Section: Generation Of Maize Doubled Haploidsmentioning

confidence: 99%

“…As mentioned, there are other methods of selection of mature kernels including oil content, near-infrared spectroscopy, and seed weight (Liu et al, 2016). The most reliable and accurate of these alternative selection methods is oil content, scoring above 90% accurate (Liu et al, 2016). High-oil inducers contain elevated levels of oil and because oil is primarily stored in the embryo, haploid kernels will contain less oil as compared to diploid kernels.…”

Section: Commentary Background Informationmentioning

confidence: 99%

“…The recent review by Liu et al (2016) provides an extensive list of uses for DH technology, which include: rapid development of inbred lines, marker-assisted backcrossing, gene stacking, population improvement, exploitation of genetic resources, among many others. DH technology does not only save time and resources, but has many other benefits.…”

Section: Generation Of Maize Doubled Haploidsmentioning

confidence: 99%

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Generation of Maize (Zea mays) Doubled Haploids via Traditional Methods

Vanous

Frei

et al. 2017

CP Plant Biology

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Commercial maize hybrid production has corroborated the usefulness of producing inbred lines; however, the delivery of new lines has always been a major time constraint in breeding programs. Traditional methods for developing inbred lines typically require 6 to 10 generations of self-pollination to obtain sufficient homozygosity. To bypass the time and costs associated with the development of inbred lines, doubled haploid (DH) systems have been widely adopted in the commercial production of maize. Within just two generations, DH systems can create completely homozygous and homogeneous lines. A typical maize DH system, utilizing anthocyanin markers R1-nj or Pl1 for haploid selection, is described in this protocol. ABSTRACTCommercial maize hybrid production has corroborated the usefulness of producing inbred lines, however, the delivery of new lines has always been a major time constraint in breeding programs. Traditional methods for developing inbred lines typically require 6-10 generations of self-pollination to obtain sufficient homozygosity. To bypass the time and costs associated with the development of inbred lines, doubled haploid (DH) systems have been widely adopted in the commercial production of maize. Within just two generations, DH systems can create completely homozygous and homogeneous lines. A typical maize DH system, utilizing anthocyanin markers R1-nj or Pl1 for haploid selection, is described in this protocol.

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A diallel analysis of a maize donor population response to in vivo maternal haploid induction: II. Haploid male fertility

et al. 2020

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Doubled haploid (DH) lines are used in maize (Zea mays L.) breeding to accelerate the breeding cycle and create homogenous inbred lines in as little as two seasons. These pure inbred lines allow breeders to quickly evaluate new cross combinations. There are two important steps in creating DH lines: (a) generation and selection of haploid progeny, and (b) genome doubling to create fertile, diploid inbreds. Colchicine is widely used to artificially double genomes in haploid plants, which is hazardous, expensive, and time consuming. In this study, three public inbred lines-A427, A637, and NK778-were found to have substantial haploid male fertility (HMF). A sixparent full diallel between these three HMF lines and three non-HMF lines was created and HMF was scored. Diallel analysis revealed significant general combining ability (GCA) estimates of up to 17% for HMF, as well as significant specific combining ability (SCA) effects of up to 25%. No significant reciprocal effects were found. Haploid male fertility is promising to be incorporated into elite maize breeding programs to potentially overcome the need of using colchicine treatments for genome doubling. Colchicine aided doubling success rates varying from almost 0 to 30%. Haploid male fertility has an advantage over artificial genome doubling, in terms of both increased success rates and decreased costs for DH line production.

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Maize Doubled Haploids

Cited by 33 publications

References 76 publications

Genome-wide association studies of doubled haploid exotic introgression lines for root system architecture traits in maize (Zea mays L.)

Genome-wide association studies of doubled haploid exotic introgression lines for root system architecture traits in maize (Zea mays L.)

Generation of Maize (Zea mays) Doubled Haploids via Traditional Methods

A diallel analysis of a maize donor population response to in vivo maternal haploid induction: II. Haploid male fertility

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