Maintenance of the Cell Morphology by MinC in Helicobacter pylori

Chiou, Pei-Yu; Luo, Cheng-Hung; Chang, Kai‐Chih; Lin, Ning

doi:10.1371/journal.pone.0071208

Cited by 13 publications

(9 citation statements)

References 29 publications

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“…The average concentration of the division inhibitor, MinC, is lowest in the middle of the cell. MinC deletion mutants form filamentous long cells but form short rods when complemented by wild‐type MinC . Here, all three genes were upregulated at pH 3.0.…”

Section: Resultsmentioning

confidence: 83%

Acid‐regulated gene expression of Helicobacter pylori: Insight into acid protection and gastric colonization

2018

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These results suggest that increasing extracellular or intracellular acidity or both are detected by the bacterium and serve as a signal to initiate increased production of protective and pathogenic factors needed to counter host defenses for persistent infection. These changes are dependent on degree of acidity and time of acid exposure, triggering a coordinated response to the environment required for colonization.

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Section: Resultsmentioning

confidence: 83%

Acid‐regulated gene expression of Helicobacter pylori: Insight into acid protection and gastric colonization

2018

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“…In addition, the abundance of cell division inhibitor (MinD) was increased in coccoids ( Supplementary Table S2 ). In H. pylori , MinD interacts with MinC to play a role in maintaining proper cell morphology and cell division as min C mutant has been demonstrated to form filamentous cells indicating a deficiency in ability to divide 40 .…”

Section: Resultsmentioning

confidence: 99%

Understanding the dimorphic lifestyles of human gastric pathogen Helicobacter pylori using the SWATH-based proteomics approach

Loke

Vilashni

et al. 2016

Sci Rep

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Helicobacter pylori may reside in the human stomach as two morphological forms: the culturable spiral form and the viable but non-culturable (VBNC) coccoid form. This bacterium transforms from spiral to coccoid under in vitro suboptimal conditions. However, both spiral and coccoid have demonstrated its infectivity in laboratory animals, suggesting that coccoid may potentially be involved in the transmission of H. pylori. To determine the relevance of the coccoid form in viability and infectivity, we compared the protein profiles of H. pylori coccoids obtained from prolonged (3-month-old) culture with that of 3-day-old spirals of two H. pylori standard strains using SWATH (Sequential Window Acquisition of all Theoretical mass spectra)-based approach. The protein profiles reveal that the coccoids retained basal level of metabolic proteins and also high level of proteins that participate in DNA replication, cell division and biosynthesis demonstrating that coccoids are viable. Most interestingly, these data also indicate that the H. pylori coccoids possess higher level of proteins that are involved in virulence and carcinogenesis than their spiral counterparts. Taken together, these findings have important implications in the understanding on the pathogenesis of H. pylori-induced gastroduodenal diseases, as well as the probable transmission mode of this bacterium.

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“…In other bacteria, a minC mutation was found to reduce motility in Helicobacter pylori, but the mutant was highly elongated in liquid culture, which the authors hypothesized was the cause of reduced motility (35). In contrast, a mutation in a minE homolog in the Gram-positive bacterium Clostridium perfringens resulted in hypermotility and hyperelongation (36).…”

Section: Discussionmentioning

confidence: 99%

Regulation of the Min Cell Division Inhibition Complex by the Rcs Phosphorelay in Proteus mirabilis

et al. 2015

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A key regulator of swarming in Proteus mirabilis is the Rcs phosphorelay, which represses flhDC, encoding the master flagellar regulator FlhD 4 C 2 . Mutants in rcsB, the response regulator in the Rcs phosphorelay, hyperswarm on solid agar and differentiate into swarmer cells in liquid, demonstrating that this system also influences the expression of genes central to differentiation. To gain a further understanding of RcsB-regulated genes involved in swarmer cell differentiation, transcriptome sequencing (RNASeq) was used to examine the RcsB regulon. Among the 133 genes identified, minC and minD, encoding cell division inhibitors, were identified as RcsB-activated genes. A third gene, minE, was shown to be part of an operon with minCD. To examine minCDE regulation, the min promoter was identified by 5= rapid amplification of cDNA ends (5=-RACE), and both transcriptional lacZ fusions and quantitative real-time reverse transcriptase (qRT) PCR were used to confirm that the minCDE operon was RcsB activated. Purified RcsB was capable of directly binding the minC promoter region. To determine the role of RcsB-mediated activation of minCDE in swarmer cell differentiation, a polar minC mutation was constructed. This mutant formed minicells during growth in liquid, produced shortened swarmer cells during differentiation, and exhibited decreased swarming motility. IMPORTANCEThis work describes the regulation and role of the MinCDE cell division system in P. mirabilis swarming and swarmer cell elongation. Prior to this study, the mechanisms that inhibit cell division and allow swarmer cell elongation were unknown. In addition, this work outlines for the first time the RcsB regulon in P. mirabilis. Taken together, the data presented in this study begin to address how P. mirabilis elongates upon contact with a solid surface. P roteus mirabilis, a Gram-negative member of the family Enterobacteriaceae, is a bacterium well known for its ability to swarm. Swarming is a specialized form of motility displayed by multicellular groups of flagellated bacteria across a solid or semisolid surface. In liquid culture, P. mirabilis exists as a peritrichously flagellated, rod-shaped cell. However, after coming into contact with a solid surface, the cells undergo differentiation into elongated, highly flagellated, multinucleate swarmer cells. Swarmer cells are 20-to 50-fold longer than vegetative cells and express thousands of flagella (1). Together, these swarmer cells form multicellular rafts, which they utilize to move across a solid surface (2). After a period of migration, the swarmer cells undergo consolidation (or dedifferentiation) and revert to vegetative rods. The repeated interchange from differentiation to consolidation is responsible for the characteristic bull's eye pattern that P. mirabilis forms on an agar plate (3, 4). Reviews on P. mirabilis swarming provide additional details on this process (5, 6).The switch from a rod-shaped cell to a swarmer cell is a complex process involving several global regulatory factors....

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Maintenance of the Cell Morphology by MinC in Helicobacter pylori

Cited by 13 publications

References 29 publications

Acid‐regulated gene expression of Helicobacter pylori: Insight into acid protection and gastric colonization

Acid‐regulated gene expression of Helicobacter pylori: Insight into acid protection and gastric colonization

Understanding the dimorphic lifestyles of human gastric pathogen Helicobacter pylori using the SWATH-based proteomics approach

Regulation of the Min Cell Division Inhibition Complex by the Rcs Phosphorelay in Proteus mirabilis

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