Maintenance of Life Cycle Stages of Fasciola hepatica in the Laboratory

Gayo, Valeria; Cancela, Mario; Acosta, Daniel

doi:10.1007/978-1-0716-0475-5_1

Cited by 6 publications

(9 citation statements)

References 7 publications

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“…is associated with Th1 cytokines (Flynn & Musah-Eroje, 2020;Moreau & Chauvin, 2010;Zhang, Moreau, Hope, Howard, Huang, & Chauvin, 2005). In an infection with F. hepatica, the initial Th1 response can act against the parasite within the peritoneal cavity and during its migration through the hepatic parenchyma (Flynn & Musah-Eroje, 2020). However, the parasite develops evasion mechanisms and modulates the host response, which is highly In sheep, resistance to Fasciola sp.…”

Section: Characterization Of Igg Antibody Production Using Elisamentioning

confidence: 99%

“…This indicates that protection against Fasciola sp. is associated with Th1 cytokines (Flynn & Musah-Eroje, 2020;Moreau & Chauvin, 2010;Zhang, Moreau, Hope, Howard, Huang, & Chauvin, 2005). In an infection with F. hepatica, the initial Th1 response can act against the parasite within the peritoneal cavity and during its migration through the hepatic parenchyma (Flynn & Musah-Eroje, 2020).…”

Section: Characterization Of Igg Antibody Production Using Elisamentioning

confidence: 99%

“…Experimental data have suggested that sheep develop little or no immune resistance to F. hepatica (Flynn & Musah-Eroje, 2020). These hosts often die from acute fascioliasis, whereas in other hosts, the parasites can remain in the organism for long periods (Dalton et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

“…In addition to these characteristics, Santa Inês sheep are highly fertile, prolific and precocious. Overall, the immune response mechanisms of domestic ruminants include early-onset eosinophilia, macrophage activation, antibody-dependent cell-mediated cytotoxicity and the action of regulatory cytokines (IL-10 and TGF-β) and Th1 and Th2 cytokines (IFN-γ and IL-4, respectively) (Mendes et al, 2013;Ruiz-Campillo et al, 2017;Flynn & Musah-Eroje, 2020;Vukman, Adams, Metz, Maurer, & O'Neill, 2013a;Vukman, Adams, & O'Neill, 2013b;Aldridge & O´Neill, 2016;Ravida et al, 2016). However, after the initial stages, the parasites develop mechanisms to subvert and modulate the innate immune response and skew it towards an adaptive response characterized by IL-4 production.…”

Section: Introductionmentioning

confidence: 99%

“…However, after the initial stages, the parasites develop mechanisms to subvert and modulate the innate immune response and skew it towards an adaptive response characterized by IL-4 production. This modulation of the T-cell response results in the development of the parasite, after which the infection becomes chronic and adult flukes become established in the bile ducts (Dalton et al, 2013;Flynn & Musah-Eroje, 2020).…”

Section: Introductionmentioning

confidence: 99%

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Immune response in Santa Inês sheep during Fasciola hepatica infection

Mendes¹,

Pereira²,

Lima³

2021

Semina: Ciênc. Agrár.

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In the present study, we describe the proliferative response and the IFN-γ, IL-10, IL-4 cytokine expression profiles in peripheral blood, and IgG production, after the experimental infection of Santa Inês sheep with 250 Fasciola hepatica metacercariae that were evaluated at 60 and 210 days post-infection(dpi). The cytokine expression profile at 60 dpi was characterized by the production of IFN-γ and IL-10, which is indicative of an initial mixed Th1/Th2 response. The modulation of the response occurred at 210 dpi with a predominance of IL-10 and IL-4 over IFN-γ. Mononuclear cells from peripheral blood stimulated with F. hepatica antigen exhibited proliferative capacity at 60 dpi, whereas the response was consistent with modulation from Th1 to Th2 in the chronic phase. The IgG antibody response was more marked at 60 days than at 210 dpi, confirming the mixed-response profile. The late modulation of the T lymphocyte response in association with the predominance of IL-10 and IL-4 at 210 dpi suggests the involvement of these cytokines after the establishment of the parasites in the bile ducts. The transition from a mixed to a regulatory response in the chronic phase was also accompanied by a reduced number of eggs per gram of feces.

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Section: Characterization Of Igg Antibody Production Using Elisamentioning

confidence: 99%