Maintenance of Cohesin at Centromeres after Meiosis I in Budding Yeast Requires a Kinetochore-Associated Protein Related to MEI-S332

Katis, V.L.; Gálová, Marta; Rabitsch, Kirsten P; Gregáň, Juraj; Nasmyth, Kim

doi:10.1016/j.cub.2004.03.001

Cited by 167 publications

(182 citation statements)

References 36 publications

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“…Similarly, homologues of Sgo1 help protect centromeric cohesion during the pre-anaphase stages of vertebrate mitosis (reviewed in Watanabe, 2005). However, the sole budding yeast Shugoshin (ScSgo1) is required to maintain sister chromatid cohesion in meiosis I but not in mitosis (Katis et al, 2004;Marston et al, 2004;Indjeian et al, 2005), demonstrating that Shugoshin function in centromere cohesion is not universal in mitosis. Other roles have been ascribed to the Shugoshin proteins, such as monitoring tension between sister-chromatids (Indjeian et al, 2005) and regulating microtubules dynamics (Salic et al, 2004;Suzuki et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Shugoshin 2 Regulates Localization of the Chromosomal Passenger Proteins in Fission Yeast Mitosis

Vanoosthuyse

Prykhozhij

Hardwick

2007

MBoC

119

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Fission yeast has two members of the Shugoshin family, Sgo1 and Sgo2. Although Sgo1 has clearly been established as a protector of centromere cohesion in meiosis I, the roles of Sgo2 remain elusive. Here we show that Sgo2 is required to ensure proper chromosome biorientation upon recovery from a prolonged spindle checkpoint arrest. Consistent with this, Sgo2 is essential for maintaining the Passenger proteins on centromeres upon checkpoint activation. Interestingly, lack of Sgo2 has a more penetrant effect on the localization of Survivin than on the two other Passenger proteins INCENP and Aurora B, and the Survivin-INCENP complex but not the INCENP-Aurora B complex is destabilized in the absence of Sgo2. Finally we show that the conserved C-terminus of Sgo2 is crucial to maintain Sgo2 and Passenger proteins localization on centromeres upon prolonged checkpoint activation. Taken together, our results demonstrate that Sgo2 is important for chromosome biorientation and that it controls docking of the Passenger proteins on chromosomes in early mitotic cells. INTRODUCTIONTo ensure the accuracy of chromosome segregation in mitosis, duplicated sister-chromatids must attach their kinetochores to microtubules emanating from opposite poles, a process referred to as chromosome biorientation. A single mal-orientated chromosome can be recognized by the spindle checkpoint that will block anaphase onset by inhibiting the activity of the anaphase-promoting complex (APC/C). Once all chromosomes are properly biorientated on the metaphase spindle, the spindle checkpoint is silenced and cells proceed through anaphase (for review, see Pinsky and Biggins, 2005).One of the best characterized roles of the kinase Aurora B is to correct defective kinetochore-microtubule attachment before anaphase onset and therefore ensure proper chromosome biorientation (Tanaka et al., 2002;Ditchfield et al., 2003;Hauf et al., 2003;Tanaka, 2005;Pinsky et al., 2006). Aurora B is also crucial for the recruitment of some spindle checkpoint components to kinetochores (Ditchfield et al., 2003;Vigneron et al., 2004). Thus Aurora B regulates both the physical connections of chromosomes onto the spindle and mitotic progression. When Aurora B activity is compromised, chromosomes mis-segregate massively, leading to aneuploidy (reviewed in Giet et al., 2005). Defective kinetochore-microtubules attachments accumulate in these cells and chromosomes never reach a proper metaphase plate (Hauf et al., 2003).Aurora B is one of the Chromosomal Passenger proteins, first identified in vertebrates as proteins sharing a complex and highly regulated localization pattern in mitosis (Earnshaw and Bernat, 1991). In particular they transfer abruptly from the inner-centromeres to the spindle midzone at the metaphase to anaphase transition. Each Chromosomal Passenger protein, Survivin, Borealin, TD60, INCENP, and Aurora B has long been recognized as major regulators of mitosis (see Vagnarelli and Earnshaw, 2004 for review). In fission yeast, homologues of only three of the Chromoso...

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Section: Introductionmentioning

confidence: 99%

Shugoshin 2 Regulates Localization of the Chromosomal Passenger Proteins in Fission Yeast Mitosis

Vanoosthuyse

Prykhozhij

Hardwick

2007

MBoC

119

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“…In Arabidopsis, syn1/dif1 plants exhibit defects in meiotic chromosome cohesion and condensation that lead to the fragmentation of chromosomes and the formation of polyads (Bhatt et al, 1999;Cai et al, 2003). Moreover, the protection of centromeric Rec8 at anaphase I appears to be mediated by the Sgo1 protein identified in S. cerevisiae (Katis et al, 2004) and widely conserved across different species (Hamant et al, 2005;Kitajima et al, 2005;Watanabe, 2005;Wang et al, 2011b).…”

mentioning

confidence: 99%

OsREC8 Is Essential for Chromatid Cohesion and Metaphase I Monopolar Orientation in Rice Meiosis

Shao

Tang²,

Wang³

et al. 2011

Plant Physiol.

119

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The successful transmission of chromosomes during mitosis and meiosis relies on the establishment and subsequent release of cohesion between replicated chromatids. Cohesion is mediated by a four-subunit structural maintenance of chromosome complex, called cohesins. REC8 is a key component of this meiotic cohesion complex in most model organisms studied to date. Here, we isolated and dissected the functions of OsREC8, a rice (Oryza sativa) REC8 homolog, using two null Osrec8 mutants. We showed that OsREC8 encodes a protein that localized to meiotic chromosomes from approximately meiotic interphase to metaphase I. Homologous pairing and telomere bouquet formation were abnormal in Osrec8 meiocytes. Furthermore, fluorescent in situ hybridization experiments on Osrec8 meiocytes demonstrated that the mutation eliminated meiotic centromeric cohesion completely during prophase I and also led to the bipolar orientation of the kinetochores during the first meiotic division and accordingly resulted in premature separation of sister chromatid during meiosis I. Immunolocalization analyses revealed that the loading of PAIR2, PAIR3, OsMER3, and ZEP1 all depended on OsREC8. By contrast, the presence of the OsREC8 signal in pair2, pair3, Osmer3, and zep1 mutants indicated that the loading of OsREC8 did not rely on these four proteins. These results suggest that OsREC8 has several essential roles in the meiotic processes.

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“…Separase becomes active again during 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 anaphase II and cleaves centromeric cohesin triggering the metaphase II-anaphase II transition. Although the levels of Sgo1 decline after meiosis I, it is still bound to chromatin until anaphase II [67] . Since centromeric cohesin is no longer protected during meiosis II, this suggests that either Sgo1 bound to chromosomes after meiosis I is not functional or activated separase is able to cleave centromeric cohesin as a result of high enzyme/substrate ratio.…”

Section: Meiosis IImentioning

confidence: 99%

How to halve ploidy: lessons from budding yeast meiosis

Kerr¹,

Sarkar²,

Arumugam³

2012

Cell. Mol. Life Sci.

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Maintenance of Cohesin at Centromeres after Meiosis I in Budding Yeast Requires a Kinetochore-Associated Protein Related to MEI-S332

Cited by 167 publications

References 36 publications

Shugoshin 2 Regulates Localization of the Chromosomal Passenger Proteins in Fission Yeast Mitosis

Shugoshin 2 Regulates Localization of the Chromosomal Passenger Proteins in Fission Yeast Mitosis

OsREC8 Is Essential for Chromatid Cohesion and Metaphase I Monopolar Orientation in Rice Meiosis

How to halve ploidy: lessons from budding yeast meiosis

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