Maintenance and Neuronal Cell Differentiation of Neural Stem Cells C17.2 Correlated to Medium Availability Sets Design Criteria in Microfluidic Systems

Wang, Bu; Jedlicka, Sabrina S.; Cheng, Xuanhong

doi:10.1371/journal.pone.0109815

Cited by 18 publications

(20 citation statements)

References 96 publications

(85 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Choice of nanomaterials, biosystems, and the corresponding nanobiohybrid construction routes. References (119)(120)(121)(122)(123)(124)(125)(126)(127)…”

Section: Supplementary Materialsmentioning

confidence: 99%

Nanobiohybrids: Materials approaches for bioaugmentation

et al. 2020

View full text Add to dashboard Cite

Nanobiohybrids, synthesized by integrating functional nanomaterials with living systems, have emerged as an exciting branch of research at the interface of materials engineering and biological science. Nanobiohybrids use synthetic nanomaterials to impart organisms with emergent properties outside their scope of evolution. Consequently, they endow new or augmented properties that are either innate or exogenous, such as enhanced tolerance against stress, programmed metabolism and proliferation, artificial photosynthesis, or conductivity. Advances in new materials design and processing technologies made it possible to tailor the physicochemical properties of the nanomaterials coupled with the biological systems. To date, many different types of nanomaterials have been integrated with various biological systems from simple biomolecules to complex multicellular organisms. Here, we provide a critical overview of recent developments of nanobiohybrids that enable new or augmented biological functions that show promise in high-tech applications across many disciplines, including energy harvesting, biocatalysis, biosensing, medicine, and robotics.

show abstract

“…Choice of nanomaterials, biosystems, and the corresponding nanobiohybrid construction routes. References (119)(120)(121)(122)(123)(124)(125)(126)(127)…”

Section: Supplementary Materialsmentioning

confidence: 99%

Nanobiohybrids: Materials approaches for bioaugmentation

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Natural organic materials often suffer from poor mechanical properties and poorly defined compositions, while synthetic organic materials in the form of hydrogels usually have poorly controlled pore sizes and structures. To gain better control of artificial scaffolds in vitro, several novel technologies in the fabrication of 3D scaffolds have recently emerged ( Figure ), such as nanoimprinting, electrospinning, 3D printing, and microfluidic devices . Nanoimprinting is a top‐down nanopatterning technique with high resolution (sub‐100 nm) and high throughput at a low cost, and well‐defined 3D nanopatterns vicon wafers, flexible polymer resists, and even nonplanar substrates .…”

Section: Scaffold Biomaterials For 3d Culture Of Stem Cellsmentioning

confidence: 99%

“…Moreover, the scaffold geometry in 3D culture systems can also affect the fate of stem cells. For example, a microfluidic device has been developed that, through a series of microchannels, supplies medium to the cells in a controlled manner that results in minimum spontaneous differentiation . In addition, hNSCs confined within a 3D channel of collagen flanked by channels of glial cell line‐derived neurotrophic factor – with signaling molecules able to diffuse between the channels – reduced the glial differentiation and enhanced the neuronal differentiation of hNSCs …”

Section: Stem Cell Biology In 3d Culturementioning

confidence: 99%

Looking into the Future: Toward Advanced 3D Biomaterials for Stem‐Cell‐Based Regenerative Medicine

et al. 2018

View full text Add to dashboard Cite

Stem-cell-based therapies have the potential to provide novel solutions for the treatment of a variety of diseases, but the main obstacles to such therapies lie in the uncontrolled differentiation and functional engraftment of implanted tissues. The physicochemical microenvironment controls the self-renewal and differentiation of stem cells, and the key step in mimicking the stem cell microenvironment is to construct a more physiologically relevant 3D culture system. Material-based 3D assemblies of stem cells facilitate the cellular interactions that promote morphogenesis and tissue organization in a similar manner to that which occurs during embryogenesis. Both natural and artificial materials can be used to create 3D scaffolds, and synthetic organic and inorganic porous materials are the two main kinds of artificial materials. Nanotechnology provides new opportunities to design novel advanced materials with special physicochemical properties for 3D stem cell culture and transplantation. Herein, the advances and advantages of 3D scaffold materials, especially with respect to stem-cell-based therapies, are first outlined. Second, the stem cell biology in 3D scaffold materials is reviewed. Third, the progress and basic principles of developing 3D scaffold materials for clinical applications in tissue engineering and regenerative medicine are reviewed.

show abstract

“…Besides user friendliness issues, 27,28 these challenges mainly stem from PDMS biocompatibility, 46,56,57 and the miniaturized culture environment impeding nutrient and gas exchange. 46,58 These issues not only affect viability, but also can lead to spontaneous differentiation of neural stem cells, preventing controlled mechanistic studies of neurodevelopment. 58 To address biocompatibility issues due to non-crosslinked oligomers and residues of the platinum catalyst used to cure the PDMS, we cleaned the device compartments through serial extractions and washes with several solvents as described previously.…”

Section: Discussionmentioning

confidence: 99%

“…46,58 These issues not only affect viability, but also can lead to spontaneous differentiation of neural stem cells, preventing controlled mechanistic studies of neurodevelopment. 58 To address biocompatibility issues due to non-crosslinked oligomers and residues of the platinum catalyst used to cure the PDMS, we cleaned the device compartments through serial extractions and washes with several solvents as described previously. 46 To address nutrient and gas exchange issues, we designed compartments with >10 μl volumes and used a PDMS membrane instead of a standard thermoplastic membrane with lower gas permeability.…”

Section: Discussionmentioning

confidence: 99%

Brain-on-a-chip model enables analysis of human neuronal differentiation and chemotaxis

et al. 2016

View full text Add to dashboard Cite

Migration of neural progenitors in the complex tissue environment of the central nervous system is not well understood. Progress in this area has the potential to drive breakthroughs in neuroregenerative therapies, brain cancer treatments, and neurodevelopmental studies. To a large extent, advances have been limited due to a lack of controlled environments recapitulating characteristics of the central nervous system milieu.Reductionist cell culture models are frequently too simplistic, and physiologically more relevant approaches such as ex vivo brain slices or in situ experiments provide little control and make information extraction difficult. Here, we present a brain-on-chip model that bridges the gap between cell culture and ex vivo/ in vivo conditions through recapitulation of self-organized neural differentiation. We use a new multi-layer silicone elastomer device, over the course of four weeks to differentiate pluripotent human (NTERA2) cells into neuronal clusters interconnected with thick axonal bundles and interspersed with astrocytes, resembling the brain parenchyma. Neurons within the device express the neurofilament heavy (NF200) mature axonal marker and the microtubule-associated protein (MAP2ab) mature dendritic marker, demonstrating that the devices are sufficiently biocompatible to allow neuronal maturation. This neuronal-glial environment is interfaced with a layer of human brain microvascular endothelial cells showing characteristics of the blood-brain barrier including the expression of zonula occludens (ZO1) tight junctions and increased trans-endothelial electrical resistance. We used this device to model migration of human neural progenitors in response to chemotactic cues within a brain-tissue setting. We show that in the presence of an environment mimicking brain conditions, neural progenitor cells show a significantly enhanced chemotactic response towards shallow gradients of CXCL12, a key chemokine expressed during embryonic brain development and in pathological tissue regions of the central nervous system. Our brain-on-chip model thus provides a convenient and scalable model of neural differentiation and maturation extensible to analysis of complex cell and tissue behaviors.

show abstract

Maintenance and Neuronal Cell Differentiation of Neural Stem Cells C17.2 Correlated to Medium Availability Sets Design Criteria in Microfluidic Systems

Cited by 18 publications

References 96 publications

Nanobiohybrids: Materials approaches for bioaugmentation

Nanobiohybrids: Materials approaches for bioaugmentation

Looking into the Future: Toward Advanced 3D Biomaterials for Stem‐Cell‐Based Regenerative Medicine

Brain-on-a-chip model enables analysis of human neuronal differentiation and chemotaxis

Contact Info

Product

Resources

About