2011
DOI: 10.1016/j.jspr.2010.10.002
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Maintaining DNA quality in stored-grain beetles caught in Lindgren funnel traps

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Cited by 13 publications
(8 citation statements)
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“…Fragmentation of DNA after immersion in propylene glycol did not differ between samples that were dehydrated and those that were not dehydrated. Generally, dehydration by substitution is recommended, because dilution by water from insect tissues can increase the risk of fragmenting DNA (Quicke et al, 1999;Stevens et al, 2011;Ferro et al, 2013). In this study, no serious fragmentation of DNA was recorded because only small amounts of tissue were preserved in the tubes.…”
Section: Discussionmentioning
confidence: 71%
See 1 more Smart Citation
“…Fragmentation of DNA after immersion in propylene glycol did not differ between samples that were dehydrated and those that were not dehydrated. Generally, dehydration by substitution is recommended, because dilution by water from insect tissues can increase the risk of fragmenting DNA (Quicke et al, 1999;Stevens et al, 2011;Ferro et al, 2013). In this study, no serious fragmentation of DNA was recorded because only small amounts of tissue were preserved in the tubes.…”
Section: Discussionmentioning
confidence: 71%
“…Comparisons of those of methods (8) and (9) revealed the effects of dehydration on fragmentation of DNA in tissues. Dilution of preservative solutions by the water from insect tissues reportedly promotes DNA fragmentation (Quicke et al, 1999;Stevens et al, 2011;Ferro et al, 2013). Each method was carried out using 12 samples.…”
Section: Introductionmentioning
confidence: 99%
“…3 days (RT) evaporation step prior to DNA extraction was ommitted as PG does not inhibit PCR; communities are highly similar and even showed higher proportion of arthropod reads and higher richness than samples conserved with >99% lab grade ethanol not to be underestimated amount of water and other compounds (Nagy 2010;Goetze and Jungbluth 2013;Borges et al 2016), but chemical interactions and their effects on medium-to long-term DNA integrity are largely unknown. In addition to high temperatures, water dilution of PG in principle leads to a generally lower DNA integrity (Stevens et al 2011;Höfer et al 2015;Patrick et al 2016;Nakamura et al 2020), since water can lead to the hydrolysis of nucleic acids. Since most reviewed studies did not indicate the nature of the propylene glycol used, or field and storage temperatures, it was difficult to compare study outcomes.…”
Section: Studymentioning
confidence: 99%
“…Some studies have reported that propylene glycol could be an effective alternative to ethanol in terms of DNA preservability (Nagy, ; Ferro & Park, ; Moreau et al, ). However, there has been insufficient evaluation of DNA preservability in propylene glycol, with a lack of cross‐taxonomic comparisons (Nagy, ; Stevens et al, ; Moreau et al, ).…”
Section: Introductionmentioning
confidence: 99%