Maintaining bovine satellite cells stemness through p38 pathway

Ding, Shijie; Swennen, Geertje; Messmer, Tobias; Gagliardi, Mick; Molin, Daniël; Li, Chunbao; Post, Mark J.

doi:10.1038/s41598-018-28746-7

Cited by 116 publications

(134 citation statements)

References 55 publications

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“…Methods and protocols for the identification and isolation of SCs, have already been widely described and only a few milligrams of muscle are now required to isolate a sufficient amount of cells to start a culture (15,16).…”

Section: Introductionmentioning

confidence: 99%

Microcarriers for Upscaling Cultured Meat Production

2020

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Section: Introductionmentioning

confidence: 99%

Microcarriers for Upscaling Cultured Meat Production

2020

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“…Bovine satellite cells (BSCs) were used to create the first clean meat prototype (Post, 2014), and their growth in bioreactors for clean meat production was recently assessed (Verbruggen et al, 2018). BSCs can be isolated from bovine carcasses (Dodson et al, 1987;Frey et al, 1995;Muroya et al, 2001;Kamanga-Sollo et al, 2004, 2008Ding et al, 2018), biopsies (Frey et al, 1995) or fetuses (Bridge et al, 1998;. The muscle tissue is ground and enzymatically digested using pronase, collagenase II or trypsin.…”

Section: Satellite Cells and Myogenesismentioning

confidence: 99%

Tissue Engineering for Clean Meat Production

Ben-Arye

Levenberg

2019

Front. Sustain. Food Syst.

189

134

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Increasing public awareness of foodborne illnesses, factory farming, and the ecological footprint of the meat industry, has generated the need for animal-free meat alternatives. In the last decade, scientists have begun to leverage the knowledge and tools accumulated in the fields of stem cells and tissue engineering toward the development of cell-based meat (i.e., clean meat). In tissue engineering, the physical and biochemical features of the native tissue can be mimicked; cells and biomaterials are integrated under suitable culture conditions to form mature tissues. More specifically, in skeletal muscle tissue engineering, a plurality of cell types can be co-cultured on a 3D scaffold to generate muscle fibers, blood vessels and a dense extracellular matrix (ECM). This review focuses on tissue engineering of skeletal muscle and the adjustments needed for clean meat development. We discuss the skeletal muscle structure and composition, and elaborate on cell types from farm animals that have the potential to recapitulate the muscle ECM, blood vessels, muscle fibers and fat deposits. We also review relevant biomaterials, primarily for fabricating scaffolds that can mimic the intramuscular connective tissues, as well as gene expression studies on the biological pathways that influence meat quality.

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“…CD56 has been mainly described as a marker for identification of quiescent human MuSCs (Alexander et al, 2016;Castiglioni et al, 2014;Uezumi et al, 2016). CD29, has been previously used for the isolation of MuSCs from mouse, pig, and cow (Ding et al, 2018;Ding et al, 2017;Maesner et al, 2016). It is a member of the integrin family and interacts with both collagen and laminin depending on its heterodimer binding partner (Hynes, 2002).…”

Section: Determination Of Reliable Positive Markers For Rat Musc Isolmentioning

confidence: 99%

“…Isolation of MuSCs has been described in mouse, human, pig, and cow (Alexander et al, 2016;Ding et al, 2018;Ding et al, 2017;Liu et al, 2015;Maesner et al, 2016;Uezumi et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Isolation of muscle stem cells from rat skeletal muscles

Sesillo

Wong

Cortez

et al. 2019

Preprint

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Background: Muscle stem cells (MuSCs) are involved in homeostatic maintenance of skeletal muscles and play a central role in muscle regeneration in response to injury. Thus, understanding MuSC autonomous properties is of fundamental importance for studies of muscle degenerative diseases and muscle plasticity. Rat, as an animal model, has been widely used in the skeletal muscle field, however an efficient approach for MuSC isolation through fluorescence-activated cell sorting from rat muscles has never been described. This work aims to develop and validate an effective protocol for MuSC isolation from rat skeletal muscles.Methods: Tibialis anterior, gastrocnemius, diaphragm, and the individual components of the pelvic floor muscle complex (coccygeus, iliocaudalis, and pubocaudalis) were harvested from female rats and digested for isolation of MuSCs. Three protocols, employing different cell surface markers (CD106, CD56, and CD29), were compared for their ability to isolate a pure MuSC population.Results: Cells obtained using the protocol that relies only on VCAM-1 (CD106) as a positive marker showed high expression of Pax7 upon isolation, ability to progress through myogenic lineage while in culture, and complete differentiation in serum deprived conditions. The protocol was further validated in other skeletal muscles proving to be reproducible. Conclusions:CD106 is an efficient marker for reliable isolation of MuSCs from a variety of rat skeletal muscles.

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Maintaining bovine satellite cells stemness through p38 pathway

Cited by 116 publications

References 55 publications

Microcarriers for Upscaling Cultured Meat Production

Microcarriers for Upscaling Cultured Meat Production

Tissue Engineering for Clean Meat Production

Isolation of muscle stem cells from rat skeletal muscles

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