Maintained right ventricular pressure overload induces ventricular–arterial decoupling in mice

Abstract

This is the peer reviewed version of the following article: Boehm, M. et al (2016), Maintained right ventricular pressure overload induces ventricular-arterial decoupling in mice. Exp Physiol., which has been published in final form at https://doi.org/10.1113/EP085963. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.eprints@whiterose.ac.uk https://eprints.whiterose.ac.uk/ Reuse Unless indicated otherwise, fulltext items are protected by copyright with all rights reserved. The copyright exception in section 29 of the Copyright, Designs and Patents Act 1988 allows the making of a single copy solely for the purpose of non-commercial research or private study within the limits of fair dealing. The publisher or other rights-holder may allow further reproduction and re-use of this version -refer to the White Rose Research Online record for this item. Where records identify the publisher as the copyright holder, users can verify any specific terms of use on the publisher's website. TakedownIf you consider content in White Rose Research Online to be in breach of UK law, please notify us by emailing eprints@whiterose.ac.uk including the URL of the record and the reason for the withdrawal request.This is an Accepted Article that has been peer-reviewed and approved for publication in the Experimental Physiology, but has yet to undergo copy-editing and proof correction. Please cite this article as an Accepted Article; doi: 10.1113/EP085963. This article is protected by copyright. All rights reserved. AbstractNew Findings: What is the central question of this study?The aim is to investigate whether complementary assessment of non-invasive ultrasound imaging along with closed chest-derived intra-cardiac pressure-volume catheterization is applicable to mice for an in-depth characterization of right ventricular (RV) function even upon maintained pressure overload. What is the main finding and its importance?Characterization

Eplerenone attenuates pathological pulmonary vascular rather than right ventricular remodeling in pulmonary arterial hypertension

“…Control animals were kept under normobaric conditions without sugen5416 injections and were fed standard diet for the entire study period (hereafter referred to as cntrl). Maintained pressure overload was surgically induced by pulmonary artery banding (PAB) as described before [ 21 – 23 ]. PAB-challenged animals were randomly assigned for either placebo (hereafter referred to as PAB) or Eplerenone (0.1% mixed in chow, hereafter referred to as Epl) therapy starting one week after disease commencement for additional two weeks.…”

“…Total pulmonary resistance index (TPRi) was calculated as RV systolic pressure divided by echocardiographically determined CI. Subsequently, intra-cardiac catheterization was performed in all animals as previously reported [ 21 , 26 ]. Terminally, all mice were euthanized by exsanguination and the RVs dissected for tissue weight measurements.…”

A companion to the preclinical common data elements for physiologic data in rodent epilepsy models. A report of the TASK3 Physiology Working Group of the ILAE/AES Joint Translational Task Force

“…With complicated arrhythmias, it may be difficult to discern a P wave from a T wave. A T wave will always follow every QRS complex, but the same is not true for P waves, which may be buried in the complex or missing from the complex altogether Frequency (heart rate): often expressed as beats/minute (bpm) Mean R‐R interval Heart rate variability (in time domain): oSDNN: Standard deviation of R‐R intervals for given epoch of time oRMSSD: root mean squared of successive differences Heart rate variability (in frequency domain): oLow frequency (LF, 0.3–0.75 Hz): represents sympathetic activity oHigh frequency (HF, 0.75–3 Hz): represents parasympathetic activity oLF/HF ratio Cardiac intervals such as P‐R interval, QT corrected (specify correction method) Measuring heart rate variability (HRV) .…”

“…A T wave will always follow every QRS complex, but the same is not true for P waves, which may be buried in the complex or missing from the complex altogether. 69,71 Parameters that can be determined: In one series of 5 unrestrained, awake Wistar rats, the mean RMSSD was 5.18 AE 1.16 msec. 72 In a study of HRV in C57BL/6J mice, the mean RMSSD was 6.1 AE 1.5 msec.…”

Levels of agreement between cardiac magnetic resonance and conductance catheter measurements of right ventricular volumes after pulmonary artery banding

“…Nonetheless, conductance catheter examinations are widely used in experimental studies to assess RV contractility and volumes. Several studies have demonstrated that pressure-volume loop-analysis of RV provides a more detailed insight into RV function than conventional catheterization (9)(10)(11)(12).…”