Main laboratory methods used for the isolation and identification of Staphylococcus spp.

Moraes, Gustavo Fernandes Queiroga; Cordeiro, Laísa Vilar; Júnior, Francisco Patrício de Andrade

doi:10.15446/rcciquifa.v50n1.95444

Cited by 11 publications

(7 citation statements)

References 52 publications

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“…According to studies; Staphylococcus sp., Streptococcus sp., and Enterococcus sp. can be differentiated by the degree of hemolysis caused by their hemolysis 39,40 .…”

Section: Hemolysis Testmentioning

confidence: 99%

The isolation and identification of Bacillus velezensis ZN-S10 from vanilla (V. planifolia), and the microbial distribution after the curing process

Manyatsi,

Lin,

Jou

2024

Preprint

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The market value of vanilla beans (Vanilla planifolia) is constantly increasing due to their natural aroma and flavor properties that improve after a curing process, where bacteria colonization plays a critical role. However, a few publications suggest that bacteria play a role in the curing process. Hence, this study aimed to isolate Bacillus sp. that could be used for fermenting V. planifolia while analyzing their role in the curing process. Bacillus velezensis ZN-S10 identified with 16S rRNA sequencing was isolated from conventionally cured V. planifolia beans. The isolate (1 mL− 1 of 10− 7 CFU mL− 1) fermented and colonized non-cured vanilla pods. PCA results revealed distinguished bacterial communities of fermented vanilla and the control group, suggesting colonization of vanilla. Phylogenetic analysis showed that ZN-S10 was the dominant Bacillus genus member and narrowly correlated to B. velezensis EM-1 and B. velezensis PMC206-1, with 78 and 73% similarity, respectively. The bacterial taxonomic profiling of cured V. planifolia had a significant relative abundance of Firmicutes, Proteobacteria, Cyanobacteria, Planctomycetes, and Bacteroidetes phyla according to the predominance. Firmicutes accounted for 55% of the total bacterial sequences, suggesting their colonization and effective fermentation roles in curing vanilla.

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“…According to studies; Staphylococcus sp., Streptococcus sp., and Enterococcus sp. can be differentiated by the degree of hemolysis caused by their hemolysis 39,40 .…”

Section: Hemolysis Testmentioning

confidence: 99%

The isolation and identification of Bacillus velezensis ZN-S10 from vanilla (V. planifolia), and the microbial distribution after the curing process

Manyatsi,

Lin,

Jou

2024

Preprint

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“…Pathogenicity [8]. Numerous virulence factors, including enterotoxins, beta, delta, and beta toxins, yeast coagulase, and white cell killer, are present in Staphylococcus aureus [9]. Many illnesses are caused by Staphylococcus aureus.…”

Section: Egyptian Journal Of Veterinary Sciences Https://ejvsjournals...mentioning

confidence: 99%

Synergistic Effectiveness of Phoenix dactylifera Seed Extract with Some Antibiotics on Staphylococcus aureus Bacteria In-vitro

Azzam,

Radif

2024

Egyptian Journal of Veterinary Sciences

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N the community and hospital settings, antibiotic resistance poses a significant 21st-century challenge. As a result, diverse therapeutic methods are imperative. Medicinal plants, known since ancient times, are gaining increased attention for treating bacterial infections. This study aimed to evaluate the inhibitory effectiveness of Phoenix dactylifera seed extract, comparing it with antibiotics, and assessing its synergy with antibiotics against S. aureus. Various extract concentrations (10%, 25%, 50%, 75%, 100%) and antibiotics (Ampicillin, Ceftriaxone, Gentamicin, Piperacillin) were employed. 37 isolated bacteria samples were grown on Muller-Hinton agar, and the well diffusion method was applied. Results revealed Gentamicin as the most effective antibiotic, averaging 29. Phoenix dactylifera extract exhibited inhibitory effects on S. aureus, with the 100% concentration yielding the best results (average of 13.667). Synergism between Gentamicin and the 100% extract concentration surpassed individual antibiotics and varied extract concentrations, achieving an average of 33.667. The combined use of antibiotic, extract, and their synergy significantly reduced germ count. Evaluation of outcomes highlighted that the 100% extract plus HLG antagonist synergy produced the greatest bacterial elimination results. These findings support the concept that bacterial illnesses can be eradicated by employing antibiotics in concert with plant extracts. Future research on different bacteria should consider various extract concentrations.

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“…S. aureus was confirmed through biochemical tests, including the coagulase, catalase, and salt mannitol agar tests (Fernandes et al 2021;Koneman et al 1997). The catalase test is a qualitative method used to detect the presence of the enzyme catalase.…”

Section: Biochemical Identification Of S Aureusmentioning

confidence: 99%

Biochemical Identification, Direct Molecular Detection, and Antimicrobial Agent Resistance of Staphylococcus Aureus Isolated from Raw Camel Milk in Al Madinah Region, Saudi Arabia

2023

Int J Vet Sci

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This study aimed to isolate and identify Staphylococcus aureus (S. aureus) in raw camel milk (RCM) samples collected from various individual farms in the Al Madinah region, Saudi Arabia, and to establish an antibiotic susceptibility profile of S. aureus isolated from RCM. Out of 115 RCM samples cultured, 52(45.2%) were isolated and detected in the tested samples as suspected S. aureus. Out of the positive samples (n=52), 96.2% were confirmed as S. aureus by the automated method. Antimicrobial resistance profiles of the confirmed isolates to 8 common antibiotics used by veterinarians in the study area were then investigated. The study revealed that the highest resistance was observed to penicillin G as the β-lactam antibiotic group (73.1%) and tetracycline as the tetracycline antibiotic group (61.5%). In comparison, the S. aureus isolates showed the highest sensitivity to clindamycin as the macrolide antibiotic group (84.6%), followed by ciprofloxacin as the quinolone antibiotic group (65.4%).

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Main laboratory methods used for the isolation and identification of Staphylococcus spp.

Cited by 11 publications

References 52 publications

The isolation and identification of Bacillus velezensis ZN-S10 from vanilla (V. planifolia), and the microbial distribution after the curing process

The isolation and identification of Bacillus velezensis ZN-S10 from vanilla (V. planifolia), and the microbial distribution after the curing process

Synergistic Effectiveness of Phoenix dactylifera Seed Extract with Some Antibiotics on Staphylococcus aureus Bacteria In-vitro

Biochemical Identification, Direct Molecular Detection, and Antimicrobial Agent Resistance of Staphylococcus Aureus Isolated from Raw Camel Milk in Al Madinah Region, Saudi Arabia

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