Main Effects of In Vitro Manipulation of Human Spermatozoa

Baldi, E; Muratori, Monica; Marchiani, Sara; Degl'Innocenti, S

doi:10.1007/978-3-030-66292-9_31

Cited by 3 publications

(7 citation statements)

References 49 publications

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“…Sperm DNA fragmentation (SDF) is a form of sperm nucleic acid damage that occurs before or after ejaculation and consists of double-stranded or single-stranded breaks [41]. At present, three main mechanisms are considered responsible for sperm DNA damage, namely impaired sperm chromatin maturation, unnatural sperm cell apoptosis, and oxidative stress [42,43].…”

Section: Correlation Between Hpv and Sperm Dna Fragmentationmentioning

confidence: 99%

“…The delivery of an undamaged paternal genome from the spermatozoa to the egg cell is essential for the correct development of the embryo [43,44]. Thus, there is a correlation between sperm DNA damage and impaired fertilization, lower embryo quality, decreased pregnancy rates, and high rates of pregnancy loss following in vitro fertilization (IVF) [41,[45][46][47][48].…”

Section: Correlation Between Hpv and Sperm Dna Fragmentationmentioning

confidence: 99%

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Human Papillomavirus and Male Infertility: What Do We Know?

Sucato,

Buttà,

Bosco

et al. 2023

IJMS

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In recent years, increasing attention has been paid to understanding the causes of infertility, which is being recognized as a growing health problem affecting large numbers of couples worldwide. Male infertility is a contributing factor in approximately 30–40% of cases, and one of its etiological causes is sexually transmitted infections (STIs). Among sexually transmitted pathogens, human papillomavirus (HPV) can contribute in various ways to the failure of spontaneous and assisted reproduction, acting in the different phases of conception, especially in the early ones. In particular, HPV infection can affect sperm DNA integrity, sperm motility, count, viability, and morphology and can induce the production of anti-sperm antibodies (ASAs). In this narrative review, we aimed to provide an overview of existing research on the potential adverse effects of HPV infection on male reproductive health. Furthermore, we analyzed how limiting the spread of the infection, particularly with gender-neutral vaccination, could be a possible therapeutic tool to counteract male and female fertility problems.

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Section: Correlation Between Hpv and Sperm Dna Fragmentationmentioning

confidence: 99%

Section: Correlation Between Hpv and Sperm Dna Fragmentationmentioning

confidence: 99%

Human Papillomavirus and Male Infertility: What Do We Know?

Sucato,

Buttà,

Bosco

et al. 2023

IJMS

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show abstract

“…ICSI, especially IMSI procedures, are more likely to take extra time. Secondly, spermatozoa may be incubated for a long time while waiting for the insemination procedure because insemination and ovulation are often not synchronized [ 17 ]. Whole in vitro manipulations may last several hours, exposing sperm to many exogenous physical and chemical factors.…”

Section: Aims Of Prolonged Non-freezing Storagementioning

confidence: 99%

“…Similar to the principle and approach of sperm cryopreservation, until now the basic strategies to prolong the lifespan of human spermatozoa in the liquid state in vitro are the partial or total replacement of seminal plasma with medium containing protectants, named as extenders, and temperature-induced metabolic restriction [ 16 ]. The attempt to perform liquid storage of semen in vitro can be traced back to the 1930s, initially in the field of artificial insemination (AI) [ 17 ] liquid storage of semen in vitro can be traced back to the 1930s, initially in the field of artificial insemination (AI) [ 18 ]. Bull and other domestic animals’ spermatozoa were chosen as the earliest research objects to meet the requirement of their reproduction and breeding.…”

Section: Introductionmentioning

confidence: 99%

Extend the Survival of Human Sperm In Vitro in Non-Freezing Conditions: Damage Mechanisms, Preservation Technologies, and Clinical Applications

Cheng

Jiang

et al. 2022

Cells

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Preservation of human spermatozoa in vitro at normothermia or hypothermia maintaining their functions and fertility for several days plays a significant role in reproductive biology and medicine. However, it is well known that human spermatozoa left in vitro deteriorate over time irreversibly as the consequence of various stresses such as the change of osmolarity, energy deficiency, and oxidative damage, leading to substantial limitations including the need for semen examinations, fertility preservation, and assisted reproductive technology. These problems may be addressed with the aid of non-freezing storage techniques. The main and most effective preservation strategies are the partial or total replacement of seminal plasma with culture medium, named as extenders, and temperature-induced metabolic restriction. Semen extenders consist of buffers, osmolytes, and antioxidants, etc. to protect spermatozoa against the above-mentioned adverse factors. Extended preservation of human spermatozoa in vitro has a negative effect on sperm parameters, whereas its effect on ART outcomes remains inconsistent. The storage duration, temperature, and pre-treatment of semen should be determined according to the aims of preservation. Advanced techniques such as nanotechnology and omics have been introduced and show great potential in the lifespan extension of human sperm. It is certain that more patients will benefit from it in the near future. This review provided an overview of the current knowledge and prospects of prolonged non-freezing storage of human sperm in vitro.

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“…Aneuploidy was at higher rates in sperms from testicular cancer gonads when compared to healthy control individuals. But the actual mechanism behind cancer and its manipulating effects on sperm characteristics has not been understood well, making the mechanism unclear (Baldi et al 2021).…”

Section: Cancer and The Sperm Characteristicsmentioning

confidence: 99%

Male Fertility Preservation: A boon for young cancer survivors

2022

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Diagnosis of any ailment especially cancer is found to be pivotal to evaluating the type of treatment that needs to be administered to man. It aids in subsequent prognosis and timely recovery in patients. When concerned with male cancer survivors, the emphasis on their fertility health is always an issue. As the numbers of survivors are increasing day by day due to the advanced medical and technological approaches, man could look with confidence to a life of ease from cancer. To review and compile all the feasible as well as relevant information about the preservation of male fertility from published resources. Reputed databases were searched for content based on specific keywords like “fertility preservation after cancer treatment", "methods of male gamete preservation", "methods of semen collection for preservation", "fertility preservation", "erectile dysfunction" and "testicular cancer and fertility". The year of publication for articles under study was restricted from 2016-2021 in most of the databases. It was found that oncologists generally recommended preservation of the male fertility before the commencement of the cancer treatment procedures. Preservation of fertility among young men should be considered in all patients before initiating any kind of prognosis related to the disease.

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Main Effects of In Vitro Manipulation of Human Spermatozoa

Cited by 3 publications

References 49 publications

Human Papillomavirus and Male Infertility: What Do We Know?

Human Papillomavirus and Male Infertility: What Do We Know?

Extend the Survival of Human Sperm In Vitro in Non-Freezing Conditions: Damage Mechanisms, Preservation Technologies, and Clinical Applications

Male Fertility Preservation: A boon for young cancer survivors

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