Main antimicrobial components of <i>Tinospora capillipes</i>, and their mode of action against <i>Staphylococcus aureus</i>

Yu, Yan; Zhang, Yi; Liang, Yi‐Zeng

doi:10.1016/j.febslet.2007.07.056

Cited by 49 publications

(27 citation statements)

References 15 publications

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“…In independent experiments, peptidyl-transferase was confirmed as the molecular target of TAN 1057 A/B, a natural compound structurally related to Bay 50-2369 (13). Metabolomic profiling is based on comprehensive analysis of global metabolism and has been enabled by recent technological advances in the quantification of cellular metabolites (2,7,14). Yu et al (14) investigated the mechanism of action of Radix Tinosporae extract by comparing the metabolomic profile of treated Staphylococcus aureus to profiles induced by nine antibiotics with known mechanisms of action.…”

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confidence: 99%

“…Yu et al (14) investigated the mechanism of action of Radix Tinosporae extract by comparing the metabolomic profile of treated Staphylococcus aureus to profiles induced by nine antibiotics with known mechanisms of action. Principal component analysis (PCA) of metabolomic profiles, obtained using high-performance liquid chromatography-mass spectrometry (HPLC-MS) data, revealed that the mechanism of action of Radix Tinosporae was similar to that of rifampin and norfloxacin (14). Techniques for multiparameter imaging-based phenotypic profiling rely on high-throughput fluorescence microscopy (15) or morphological analysis (16) and have shown great promise in drug discovery research (2,7).…”

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Phenotypic Profiling of Antibiotic Response Signatures in Escherichia coli Using Raman Spectroscopy

Athamneh

Alajlouni

Wallace

et al. 2014

Antimicrob Agents Chemother

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bIdentifying the mechanism of action of new potential antibiotics is a necessary but time-consuming and costly process. Phenotypic profiling has been utilized effectively to facilitate the discovery of the mechanism of action and molecular targets of uncharacterized drugs. In this research, Raman spectroscopy was used to profile the phenotypic response of Escherichia coli to applied antibiotics. The use of Raman spectroscopy is advantageous because it is noninvasive, label free, and prone to automation, and its results can be obtained in real time. In this research, E. coli cultures were subjected to three times the MICs of 15 different antibiotics (representing five functional antibiotic classes) with known mechanisms of action for 30 min before being analyzed by Raman spectroscopy (using a 532-nm excitation wavelength). The resulting Raman spectra contained sufficient biochemical information to distinguish between profiles induced by individual antibiotics belonging to the same class. The collected spectral data were used to build a discriminant analysis model that identified the effects of unknown antibiotic compounds on the phenotype of E. coli cultures. Chemometric analysis showed the ability of Raman spectroscopy to predict the functional class of an unknown antibiotic and to identify individual antibiotics that elicit similar phenotypic responses. Results of this research demonstrate the power of Raman spectroscopy as a cellular phenotypic profiling methodology and its potential impact on antibiotic drug development research.

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Phenotypic Profiling of Antibiotic Response Signatures in Escherichia coli Using Raman Spectroscopy

Athamneh

Alajlouni

Wallace

et al. 2014

Antimicrob Agents Chemother

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“…231 Gene expression profiling with either the entire transcriptome 232,233 or a subset of reporter genes 234,235 has also been used to predict modes of action for natural products. However, because these transcriptomic screens are still relatively costly, there is a great interest in applying metabolomics analyses to predict natural product modes of action using either natural product extracts 236 or purified compounds. 237-242 Vincent et al have recently shown untargeted metabolomics can effectively identify compound modes of action when specific metabolic pathways are the primary drug target.…”

Section: Investigations Of Secondary Metabolite Bioactivitymentioning

confidence: 99%

Comparative mass spectrometry-based metabolomics strategies for the investigation of microbial secondary metabolites

2017

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The labor-intensive process of microbial natural product discovery is contingent upon identifying discrete secondary metabolites of interest within complex biological extracts, which contain inventories of all extractable small molecules produced by an organism or consortium. Historically, compound isolation prioritization has been driven by observed biological activity and/or relative metabolite abundance and followed by dereplication via accurate mass analysis. Decades of discovery using variants of these methods has generated the natural pharmacopeia but also contributes to recent high rediscovery rates. However, genomic sequencing reveals substantial untapped potential in previously mined organisms, and can provide useful prescience of potentially new secondary metabolites that ultimately enables isolation. Recently, advances in comparative metabolomics analyses have been coupled to secondary metabolic predictions to accellerate bioactivity and abundance-independent discovery work flows. In this review we will discuss the various analytical and computational techniques that enable MS-based metabolomic applications to natural product discovery and discuss the future prospects for comparative metabolomics in natural product discovery.

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“…Jatrorrhizine is a kind of quaternary protoberberine alkaloids (QPA) and was considered as one of the active constituents of Coptis chinensis Franch [5,6]. Jatrorrhizine has multiple bioactivities, such as hypoglycemic [7], antimicrobial [8], and antioxidant activities [9]. In recent years, berberine derivatives with various heterocyclic rings, such as thiophene, pyrrole, piperidine, and carbazole, were demonstrated as both acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) inhibitors [10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Synthesis and Biological Evaluation of Novel Jatrorrhizine Derivatives with Amino Groups Linked at the 3-Position as Inhibitors of Acetylcholinesterase

Jiang

Zhang

Xiong

et al. 2017

Journal of Chemistry

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Jatrorrhizine was considered as one of the active constituents of Coptis chinensis Franch. Herein, jatrorrhizine derivatives with substituted amino groups linked at the 3-position were designed, synthesized, and biologically evaluated as inhibitors of acetylcholinesterase. Jatrorrhizine derivatives inhibited the activity of acetylcholinesterase (AChE) to a greater extent than the lead compound jatrorrhizine. All these jatrorrhizine derivatives were proved to be potent inhibitors of acetylcholinesterase (AChE) with submicromolar IC 50 values, but less sensitive to butyrylcholinesterase (BuChE), which suggests that these jatrorrhizine derivatives are selective for AChE/BuChE. Compound g gave the most potent inhibitor activity for AChE (IC 50 = 0.301 M), which is greater than the lead compound jatrorrhizine. All these results demonstrated that these jatrorrhizine derivatives are potential inhibitors for AChE.

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Main antimicrobial components of Tinospora capillipes, and their mode of action against Staphylococcus aureus

Cited by 49 publications

References 15 publications

Phenotypic Profiling of Antibiotic Response Signatures in Escherichia coli Using Raman Spectroscopy

Phenotypic Profiling of Antibiotic Response Signatures in Escherichia coli Using Raman Spectroscopy

Comparative mass spectrometry-based metabolomics strategies for the investigation of microbial secondary metabolites

Synthesis and Biological Evaluation of Novel Jatrorrhizine Derivatives with Amino Groups Linked at the 3-Position as Inhibitors of Acetylcholinesterase

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