Main and Minor Types of Collagens in the Articular Cartilage: The Role of Collagens in Repair Tissue Evaluation in Chondral Defects

Alcaide‐Ruggiero, Lourdes; Molina-Hernández, Verónica; Granados, Miguel Ángel Núñez; Domínguez, Juan M.

doi:10.3390/ijms222413329

Cited by 59 publications

(63 citation statements)

References 158 publications

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“…On the other hand, the downregulated genes in the FGF10‐treated blastemas contained genes associated with chondrogenesis; the ten genes that showed the largest fold change in expression among the downregulated genes in the FGF10‐treated blastemas contained collagens ( collagen type III alpha 1 and collagen type XII alpha 1 ; col3a1.L , col12a1.L ), versican.L ( vcan.L ), and zinc finger homeodomain 4.S ( zfhx4.S ) (Table S3 and Figure S4). Collagen is a main structural protein in the extracellular matrix found in connective tissue such as cartilage (Alcaide‐Ruggiero et al, 2021). vcan is reported to facilitate chondrogenesis in mice (Choocheep et al, 2010).…”

Section: Resultsmentioning

confidence: 99%

Cellular responses in the FGF10‐mediated improvement of hindlimb regenerative capacity in Xenopus laevis revealed by single‐cell transcriptomics

et al. 2022

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Xenopus laevis tadpoles possess regenerative capacity in their hindlimb buds at early developmental stages (stages ~52–54); they can regenerate complete hindlimbs with digits after limb bud amputation. However, they gradually lose their regenerative capacity as metamorphosis proceeds. Tadpoles in late developmental stages regenerate fewer digits (stage ~56), or only form cartilaginous spike without digits or joints (stage ~58 or later) after amputation. Previous studies have shown that administration of fibroblast growth factor 10 (FGF10) in late‐stage (stage 56) tadpole hindlimb buds after amputation can improve their regenerative capacity, which means that the cells responding to FGF10 signaling play an important role in limb bud regeneration. In this study, we performed single‐cell RNA sequencing (scRNA‐seq) of hindlimb buds that were amputated and administered FGF10 by implanting FGF10‐soaked beads at a late stage (stage 56), and explored cell clusters exhibiting a differential gene expression pattern compared with that in controls treated with phosphate‐buffered saline. The scRNA‐seq data showed expansion of fgf8‐expressing cells in the cluster of the apical epidermal cap of FGF10‐treated hindlimb buds, which was reported previously, indicating that the administration of FGF10 was successful. On analysis, in addition to the epidermal cluster, a subset of myeloid cells and a newly identified cluster of steap4‐expressing cells showed remarkable differences in their gene expression profiles between the FGF10‐ or phosphate‐buffered saline‐treatment conditions, suggesting a possible role of these clusters in improving the regenerative capacity of hindlimbs via FGF10 administration.

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Section: Resultsmentioning

confidence: 99%

Cellular responses in the FGF10‐mediated improvement of hindlimb regenerative capacity in Xenopus laevis revealed by single‐cell transcriptomics

et al. 2022

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“…Type III collagen is often co-localized with type I within the same fibril ( Dehne et al, 2010 ), for this reason, it is also considered as a negative marker. However, its exact role in chondrogenesis remains unclear because contrasting evidence on its protective or pathological functions after injuries is reported ( Alcaide-Ruggiero et al, 2021 ). The development of a stellate or fibroblast-like phenotype during culture compromises clinical outcome of potential regenerative therapy and is a major issue for application of hBM-MSCs in cartilage defect repair ( Li et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

3D in-vitro cultures of human bone marrow and Wharton’s jelly derived mesenchymal stromal cells show high chondrogenic potential

Lamparelli

Ciardulli

Giudice

et al. 2022

Front. Bioeng. Biotechnol.

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In this study, chondrogenic potentials of 3D high-density cultures of Bone Marrow (BM) and Wharton’s Jelly (WJ)-derived mesenchymal stromal cells (MSCs) was investigated by chondrogenesis- and cytokine-related gene expression over a 16-day culture period supplemented with human transforming growth factor (hTGF)-β1 at 10 ng/ml. In BM-MSC 3D models, a marked upregulation of chondrogenesis-related genes, such as SOX9, COL2A1, and ACAN (all p < 0.05) and formation of spherical pellets with structured type II collagen fibers were observed. Similarly, WJ-based high-density culture appeared higher in size and more regular in shape, with a significant overexpression of COL2A1 and ACAN (all p < 0.05) at day 16. Moreover, a similar upregulation trend was documented for IL-6 and IL-10 expression in both BM and WJ 3D systems. In conclusion, MSC-based high-density cultures can be considered a promising in vitro model of cartilage regeneration and tissue engineering. Moreover, our data support the use of WJ-MSCs as a valid alternative for chondrogenic commitment of stem cells in regenerative medicine.

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“…Type II collagen fibers are thin, dense, and parallel to the joint surface providing stretch enhancement for highly hydrated articular cartilage. [ 21 ]…”

Section: Structure Of Cartilage Tissuementioning

confidence: 99%

Cartilaginous Organoids: Advances, Applications, and Perspectives

Wang

et al. 2022

Advanced NanoBiomed Research

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Cartilage is a nonvascular and non‐neural, mesoderm‐derived tissue. Cartilage repair, especially articular cartilage repair and reconstruction, has always been a difficult problem in bone tissue engineering. The unique biological characteristics and mechanical structure of articular cartilage make articular cartilage reconstruction a great challenge. Currently, it is extremely difficult to construct artificial cartilage tissue with all the information of natural cartilage in vitro, which greatly restricts the development of cartilage tissue engineering. Organoid technology is a booming research topic in recent years, which can mimic the complex biological characteristics of organs or tissues in vivo by constructing them in vitro through tissue engineering techniques. Cartilage tissues constructed using organoid technology may provide us with a novel tool to study cartilage development, cartilage pathological processes, and cartilage repair. This review introduces the concept and research progress of cartilage organoids and focuses on the composition and cultivation methods of cartilage organoids and application scenarios, besides giving an outlook on the limitations of present research.

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Main and Minor Types of Collagens in the Articular Cartilage: The Role of Collagens in Repair Tissue Evaluation in Chondral Defects

Cited by 59 publications

References 158 publications

Cellular responses in the FGF10‐mediated improvement of hindlimb regenerative capacity in Xenopus laevis revealed by single‐cell transcriptomics

Cellular responses in the FGF10‐mediated improvement of hindlimb regenerative capacity in Xenopus laevis revealed by single‐cell transcriptomics

3D in-vitro cultures of human bone marrow and Wharton’s jelly derived mesenchymal stromal cells show high chondrogenic potential

Cartilaginous Organoids: Advances, Applications, and Perspectives

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