2013
DOI: 10.1111/1750-3841.12061
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Maillard‐Reaction‐Induced Modification and Aggregation of Proteins and Hardening of Texture in Protein Bar Model Systems

Abstract: The hardening of high-protein bars causes problems in their acceptability to consumers. Maillard reaction is one of the mechanisms contributing to the hardening of bar matrix, particularly for the late stage of storage. The replacement of reducing sugars with nonreducing ingredients such as sugar alcohols in the formula will minimize the changes in texture.

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Cited by 48 publications
(38 citation statements)
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“…SDS and electrophoresis staining was used to characterize the MRPs. Samples were heated at 70–85 °C (MRPs of B 1 /B 2 ) and then mixed with an equal volume of sample buffer . Samples (10 µL) were loaded onto a 5% (pH 6.8) stacking gel and a 10% (pH 8.8) separating gel was used (Actuator Power supplies PowerPac Series; Bio‐Rad, Hercules, CA, USA).…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…SDS and electrophoresis staining was used to characterize the MRPs. Samples were heated at 70–85 °C (MRPs of B 1 /B 2 ) and then mixed with an equal volume of sample buffer . Samples (10 µL) were loaded onto a 5% (pH 6.8) stacking gel and a 10% (pH 8.8) separating gel was used (Actuator Power supplies PowerPac Series; Bio‐Rad, Hercules, CA, USA).…”
Section: Methodsmentioning
confidence: 99%
“…Samples were heated at 70-85 ∘ C (MRPs of B 1 /B 2 ) and then mixed with an equal volume of sample buffer. 21 Samples (10 μL) were loaded onto a 5% (pH 6.8) stacking gel and a 10% (pH 8.8) separating gel was used (Actuator Power supplies Power-Pac Series; Bio-Rad, Hercules, CA, USA). Protein was stained with Coomassie blue, and the glycoprotein was stained with periodic acid-Schiff reagent when electrophoresis was finished.…”
Section: Sds-pagementioning
confidence: 99%
“…Hal ini diduga disebabkan oleh protein ikan membantu dalam meningkatkan kapasitas rehidrasi bahan. Selain itu, reaksi Maillard yang terjadi selama pengeringan membentuk struktur kompleks yang menyebabkan struktur bahan lebih kompak (Zhou et al, 2013).…”
Section: Karakteristik Rehidrasiunclassified
“…WHC pada tekwan kering diduga dipengaruhi oleh struktur penyusutan protein fibril, ikatan cross-link kovalen antar agregat protein, dan ikatan disulfida intermolekul. Zhou et al (2013) menyatakan bahwa pembentukan ikatan intermolekul protein dan pati termediasi melalui reaksi Maillard.…”
Section: Water Holding Capacityunclassified
“…Melanoidins in particular, the brown polymers which are the final product of the Maillard reaction, are known to be present in large amounts in roasted nuts . Indeed has been shown that in protein bars high molecular weight Maillard reaction products can result in texture hardening (Zhou, Guo, Liu, Liu, & Labuza, 2013). It is likely that the presence reducing sugars (endogenous or added in the maceration medium) increases the rate of Maillard reaction, and therefore leads to a higher production of melanoidins which in turn affects the texture (hardness).…”
mentioning
confidence: 99%