Magnolol enhances adipocyte differentiation and glucose uptake in 3T3-L1 cells

Choi, Sooyoung; Cha, Byung‐Yoon; Lee, Young-Sil; Yonezawa, Takayuki; Teruya, Toshiaki; Nagai, Kazuo; Woo, Je‐Tae

doi:10.1016/j.lfs.2009.04.001

Cited by 95 publications

(85 citation statements)

References 36 publications

(33 reference statements)

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“…For example, nobiletin, a polymethoxylated fl avone found in certain citrus fruits, activates cAMP-responsive element binding protein (CREB) and extracellular signal-regulated kinase (ERK), thereby leading to an enhanced adipogenesis through activation of signaling cascades including C/EBPβ and PPARγ without PPARγ ligand activity (Saito et al, 2007). Magnolol, obtained from the medicinal herb Magnolia offi cinalis, failed to increase PPARγ gene expression, but did increase the expression of transcriptional target genes of PPARγ, such as aP2 and C/ EBPα in fully differentiated 3T3-L1 adipocytes, exhibiting weak PPARγ ligand activity (Choi et al, 2009). Emodin, one of the main active components in the root and rhizome of Rheum palmatum L, exhibited a very high binding affi nity to PPARγ .…”

Section: Discussionmentioning

confidence: 99%

“…The activation of PPARγ by its ligands is a key process in adipocyte differentiation and diverse natural compounds have been reported to act as PPARγ ligands Hassan et al, 2007;Choi et al, 2009;Shin et al, 2009;Saito et al, 2009;Yang et al, 2007). Thus, whether IOWE served as a PPARγ ligand was tested using the luciferase reporter system.…”

Section: Iowe Is Not a Pparγ Ligandmentioning

confidence: 99%

“…To date, numerous compounds that promote adipocyte differentiation have been found, including lecithin (Zhang et al, 2009), cilostazol , sakuranetin (Saito et al, 2008), phloretin (Hassan et al, 2007), magnolol (Choi et al, 2009), catechin (Shin et al, 2009), ginsenosides (Niu et al, 2009;Shang et al, 2007), fl avanone (Saito et al, 2009), raloxifene (Murase et al, 2006), progestins (Rondinone et al,1992), diallyl disulfi de (Lee et al, 2007b), emodin and nobiletin (Saito et al, 2007). More recently, Kim et al (2008) explored a wide variety of natural marine products for screening the PPARα/γ transcriptional activity, thereby fi nding two novel PPARα/γ dual agonists, sargaquinoic acid and sargahydroquinoic acid.…”

Section: Introductionmentioning

confidence: 99%

“…PPARγ ligands are known to increase glucose transport by insulin-sensitive tissues by regulating the expression of several genes involved in glucose metabolism (Choi et al, 2009;Ribon et al, 1998). Glucose uptake in insulin-sensitive tissues is directly associated with a number of glucose transporters (Choi et al, 2009;Hansen et al, 1995;Gnudi et al, 1995;Wood and Trayhurn, 2003).…”

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confidence: 99%

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Extract of Chaga mushroom (Inonotus obliquus) stimulates 3t3‐l1 adipocyte differentiation

Joo

Kim

Yun

2010

Phytotherapy Research

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Chaga mushroom (Inonotus obliquus) has long been used as a folk medicine due to its numerous biological functions such as antibacterial, antiallergic, antiinflammatory and antioxidative activities. In the present study, it was found that the I. obliquus hot water extract (IOWE) activated adipogenesis of 3T3-L1 preadipocytes. Even in the absence of adipogenic stimuli by insulin, the IOWE strongly induced adipogenesis of 3T3-L1 preadipocytes. The major constituent of IOWE was glucose-rich polysaccharides with a molecular mass of 149 kDa. IOWE enhanced the differentiation of 3T3-L1 preadipocytes, increasing TG (triacylglycerol) accumulation that is critical for acquisition of the adipocyte phenotype, in a dose-dependent manner. IOWE stimulated gene expression of C/EBPα (CCAAT/enhancer-binding protein α) and PPARγ (peroxisome proliferator-activated receptors γ) during adipocyte differentiation, and induced the expression of PPARγ target genes such as aP2 (adipocyte protein 2), LPL (lipoprotein lipase) and CD36 (fatty acid translocase). Immunoblot analysis revealed that IOWE increased the expression of adipogenic makers such as PPARγ and GLUT4 (glucose transporter 4). The luciferase reporter assay demonstrated that IOWE did not exhibit PPARγ ligand activity. Although these results require further investigation, the ability of natural mushroom product to increase PPARγ transcriptional activities may be expected to be therapeutic targets for dyslipidemia and type 2 diabetes.

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Section: Discussionmentioning

confidence: 99%

Section: Iowe Is Not a Pparγ Ligandmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Extract of Chaga mushroom (Inonotus obliquus) stimulates 3t3‐l1 adipocyte differentiation

Joo

Kim

Yun

2010

Phytotherapy Research

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“…These compounds exhibit anti-cancer, antiinflammatory, anti-angiogenic, and/or cardioprotective activities, as well as anti-oxidative effects [76][77][78] . They bind to PPARγ with micromolar affinities and display weak agonistic activity; magnolol was reported to induce adipogenesis and glucose uptake in 3T3-L1 cells [79] . It can additionally bind RXRα with a micromolar affinity and was described as a dual agonist of PPARγ and RXRα.…”

Section: Other Environmental Pparγ Ligandsmentioning

confidence: 99%

A structural perspective on nuclear receptors as targets of environmental compounds

Delfosse¹,

Maire²,

Balaguer³

et al. 2014

Acta Pharmacol Sin

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Nuclear receptors (NRs) are members of a large superfamily of evolutionarily related transcription factors that control a plethora of biological processes. NRs orchestrate complex events such as development, organ homeostasis, metabolism, immune function, and reproduction. Approximately one-half of the 48 human NRs have been shown to act as ligand-regulated transcription factors and respond directly to a large variety of endogenous hormones and metabolites that are generally hydrophobic and small in size (eg, retinoic acid or estradiol). The second half of the NR family comprises the so-called orphan receptors, for which regulatory ligands are still unknown or may not exist despite the presence of a C-terminal ligand-binding domain, which is the hallmark of all NRs. Several chemicals released into the environment (eg, bisphenols, phthalates, parabens, etc) share some physicochemical properties with natural ligands, allowing them to bind to NRs and activate or inhibit their action. Collectively referred to as endocrine disruptors or endocrine-disrupting chemicals (EDCs), these environmental pollutants are highly suspected to cause a wide range of developmental, reproductive, neurological, or metabolic defects in humans and wildlife. Crystallographic studies are revealing unanticipated mechanisms by which chemically diverse EDCs interact with the ligand-binding domain of NRs. These studies thereby provide a rational basis for designing novel chemicals with lower impacts on human and animal health. In this review, we provide a structural and mechanistic view of endocrine disrupting action using estrogen receptors α and β, (ERα/β), peroxisome proliferator activated receptor γ (PPARγ), and their respective environmental ligands as representative examples.

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Honokiol and magnolol stimulate glucose uptake by activating PI3K‐dependent Akt in L6 myotubes

et al. 2012

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Honokiol and magnolol, ingredients of Magnolia officinalis, which is used in traditional Chinese and Japanese medicines, have been reported to have antioxidant, anticancer, and antiangiogenic effects. Effects of these compounds on glucose metabolism in adipocytes have also been reported. However, their effects on skeletal muscle glucose uptake and the underlying molecular mechanisms are still unknown. Here, we investigated the direct effects and signaling pathways activated by honokiol and magnolol in skeletal muscle cells using L6 myotubes. We found that honokiol and magnolol dose-dependently acutely stimulated glucose uptake without synergistic effects of combined administration in L6 myotubes. Treatment with honokiol and magnolol also stimulated glucose transporter-4 translocation to the cell surface. Honokiol- and magnolol-stimulated glucose uptake was blocked by the phosphatidylinositol-3 kinase inhibitor, wortmannin. Both honokiol and magnolol stimulated Akt phosphorylation, a key element in the insulin signaling pathway, which was completely inhibited by wortmannin. These results suggest that honokiol and magnolol might have beneficial effects on glucose metabolism by activating the insulin signaling pathway.

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Magnolol enhances adipocyte differentiation and glucose uptake in 3T3-L1 cells

Cited by 95 publications

References 36 publications

Extract of Chaga mushroom (Inonotus obliquus) stimulates 3t3‐l1 adipocyte differentiation

Extract of Chaga mushroom (Inonotus obliquus) stimulates 3t3‐l1 adipocyte differentiation

A structural perspective on nuclear receptors as targets of environmental compounds

Honokiol and magnolol stimulate glucose uptake by activating PI3K‐dependent Akt in L6 myotubes

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