The subtropical broadleaved humid forest of Meghalaya, northeast India are characterised by small remnant patches. The major threats to the existing patches are anthropogenic activities such as encroachment of forest area, mining, extraction of forest resources, grazing and forest fire. The present study was carried out at Jarain and adjoining areas of Jaintia Hills in Meghalaya, northeast India to identify the current human disturbances and assess the floristic composition and structure of subtropical broad leaved forest along a fragment size gradient. Floristic sampling was carried out by laying 24 plots (20mx100m) across 10 forest fragments covering a sampled area of 4.8 ha. A total of 160 woody species (≥5cm dbh) belonging to 105 genera and 54 families were enumerated from all the studied fragments. The species richness was 69 in Small, 75 in Medium, 76 in Very Large (VL) and 77 in Large (L) fragment classes. In this study, Pearson's correlation analysis was performed to analyse the relationship between area, disturbance and phytosociological attributes. The results showed that the stand density increased (r = 0.71, p=0.01), while basal area decreased with disturbance (r = -0.74, p= 0.01). The density was high in 5-15cm dbh class that gradually declined with the increase in diameter. The basal area was high in >66cm dbh class in Very Large fragments whereas in small patches, the values were higher in 16-25cm dbh classes. The forests fragments under study also have a number of rare, endemic and threatened species. Therefore, it is suggested that the entire landscape be brought under the protected area network due to high species diversity.