Magnoflorine attenuates inflammatory responses in RA by regulating the PI3K/Akt/NF-κB and Keap1-Nrf2/HO-1 signalling pathways in vivo and in vitro

Shen, Yue; Fan, Xinting; Qu, Yuhan; Tang, Min; Huang, Yuehui; Peng, Yi; Fu, Qiang

doi:10.1016/j.phymed.2022.154339

Cited by 19 publications

(12 citation statements)

References 70 publications

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“…5 D, Nrf2 nuclear protein/Nrf2 cytoplasmic protein was increased, indicating that tangeretin was able to promote Nrf2 nuclear translocation. Under normal conditions, Nrf2 binds to Keap1, which anchors Nrf2 in the cytoplasm and allowing its continuous degradation via the proteasome during ubiquitin-mediated processed ( Hong et al, 2022 ; Puppala et al, 2022 ; Shen et al, 2022b ). Upon binding to tangeretin, Nrf2 dissociates from Keap1 and Nrf2 translocated to the nucleus.…”

Section: Resultsmentioning

confidence: 99%

Characterization of tangeretin as an activator of nuclear factor erythroid 2-related factor 2/antioxidant response element pathway in HEK293T cells

Liang

et al. 2023

Current Research in Food Science

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Section: Resultsmentioning

confidence: 99%

Characterization of tangeretin as an activator of nuclear factor erythroid 2-related factor 2/antioxidant response element pathway in HEK293T cells

Liang

et al. 2023

Current Research in Food Science

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“…The Keap1/Nrf2/HO‐1 signaling pathway, a classic pathway related to oxidative stress, has been reported to be involved in many diseases, including osteoclastogenesis, 34 type 1 diabetes, 35 and nephrotoxicity 36 . In recent years, increasingly more studies have focused on the correlation of the Keap1/Nrf2/HO‐1 signaling pathway with inflammatory response 17,37,38 . In addition, a recent study found that the Keap1/Nrf2/HO‐1 signaling pathway is involved in LPS‐induced epithelial cell inflammatory damage 39 .…”

Section: Discussionmentioning

confidence: 99%

Oxycodone alleviates mifepristone‐stimulated human endometrial stromal cell injury by activating the Keap1/Nrf2/HO‐1 signaling pathway

Zhu,

Yao,

Shen

2023

Immunity Inflam & Disease

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BackgroundEndometrial injury is a common disease in women caused by intrauterine inflammation, infections, and endocrine disorders. Human endometrial stromal cells (hEndoSCs) can maintain endometrial homeostasis and play an important role in repairing endometrial injury. Mifepristone, a steroidal anti‐progesterone drug, is widely used in the field of reproductive medicine worldwide. Mifepristone‐induced hEndoSC injury has been used to study endometrial injury in vitro. At present, the pathogenesis and potential regulatory mechanisms of oxycodone in endometrial injury remain unknown.AimsWe aimed to evaluate the functions of oxycodone in mifepristone‐stimulated hEndoSC injury and analyze its potential molecular mechanism.Materials & MethodshEndoSC viability, cytotoxicity, and apoptosis were analyzed using the methyl thiazolyl tetrazolium assay, the lactate dehydrogenase assay, and flow cytometry, respectively. Furthermore, the levels of cleaved‐Caspase3, Keap1, Nrf2, HO‐1, and NQO1 were assessed using reverse transcription quantitative polymerase chain reaction and western blot analysis, and the release of inflammatory cytokines was determined using the enzyme‐linked immunosorbent assay.ResultsWe observed that oxycodone had no adverse effects on hEndoSCs; rather, it protected hEndoSCs against mifepristone‐induced endometrial damage, as confirmed by the enhanced cell viability, reduced number of apoptotic cells, decreased Caspase3 activity and inflammatory cytokine secretion, and increased Keap1/Nrf2/HO‐1 pathway‐related protein expression. In addition, we found that the protective effects of oxycodone on mifepristone‐induced hEndoSC injury were inhibited by ML385 (a Keap1/Nrf2/HO‐1 inhibitor).ConclusionIn summary, we confirmed that oxycodone alleviates mifepristone‐induced hEndoSC injury by activating the Keap1/Nrf2/HO‐1 signaling pathway.

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“…Haque et al reported that magnoflorine enhanced pro-inflammatory responses through producing of TNF-α and IL-1β ( Haque et al, 2018 ). However, Shen et al showed that magnoflorine attenuated inflammatory responses by reducing inflammatory cytokines, such as IL-6 and IL-8 ( Shen et al, 2022 ). Further research is needed to confirm this opposite conclusion.…”

Section: Anti-inflammatory Activitiesmentioning

confidence: 99%

“…Additionally, apigenin, luteolin, quercetin and kaempferol had an inhibitory effect on NO production stimulated by LPS in RAW 264.7 macrophage cells ( Lee and Kim, 2010 ). In vitro studies showed that magnoflorine, isoliquiritigenin, quercetin, kaempferol, luteolin and apigenin suppressed the level of iNOS ( Sun et al, 2020 ; Lin et al, 2021 ) and the mechanism may be associated with PI3K/Akt/NF-κB signaling axis and the Keap1-Nrf2/HO-1 signaling pathway ( Shen et al, 2022 ). Furthermore, iNOS was inhibited markedly by quercetin in CIA mice ( Yang et al, 2018b ) and suppressed by hyperoside in fibroblast-like synoviocytes (FLS) ( Fu.…”

Section: Anti-inflammatory Activitiesmentioning

confidence: 99%

“…et al, 2020 ), NF-κB signaling pathway ( Lou et al, 2015 ; Jin et al, 2016 ), PI3K/Akt pathways ( Hou et al, 2009 ) and JAK/STAT signaling pathways ( Lou et al, 2015 ). Magnoflorine inhibited migration, invasion, proliferation and induced apoptosis and cell cycle arrest of RAFLS via inhibiting the PI3K/Akt/NF-κB axis signaling pathway and activating the Keap1-Nrf2/HO-1 signaling pathway ( Shen et al, 2022 ).…”

Section: Effects On Fls Proliferation Migration and Apoptosismentioning

confidence: 99%

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Epimedii Herba: An ancient Chinese herbal medicine in the prevention and treatment of rheumatoid arthritis

Liu-bo

Yan

et al. 2022

Front. Chem.

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Rheumatoid arthritis (RA) is a chronic, progressive inflammatory and systemic autoimmune disease resulting in severe joint destruction, lifelong suffering and considerable disability. Diverse prescriptions of traditional Chinese medicine (TCM) containing Epimedii Herba (EH) achieve greatly curative effects against RA. The present review aims to systemically summarize the therapeutic effect, pharmacological mechanism, bioavailability and safety assessment of EH to provide a novel insight for subsequent studies. The search terms included were “Epimedii Herba”, “yinyanghuo”, “arthritis, rheumatoid” and “Rheumatoid Arthritis”, and relevant literatures were collected on the database such as Google Scholar, Pubmed, Web of Science and CNKI. In this review, 15 compounds from EH for the treatment of RA were summarized from the aspects of anti-inflammatory, immunoregulatory, cartilage and bone protective, antiangiogenic and antioxidant activities. Although EH has been frequently used to treat RA in clinical practice, studies on mechanisms of these activities are still scarce. Various compounds of EH have the multifunctional traits in the treatment of RA, so EH may be a great complementary medicine option and it is necessary to pay more attention to further research and development.

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Magnoflorine attenuates inflammatory responses in RA by regulating the PI3K/Akt/NF-κB and Keap1-Nrf2/HO-1 signalling pathways in vivo and in vitro

Cited by 19 publications

References 70 publications

Characterization of tangeretin as an activator of nuclear factor erythroid 2-related factor 2/antioxidant response element pathway in HEK293T cells

Characterization of tangeretin as an activator of nuclear factor erythroid 2-related factor 2/antioxidant response element pathway in HEK293T cells

Oxycodone alleviates mifepristone‐stimulated human endometrial stromal cell injury by activating the Keap1/Nrf2/HO‐1 signaling pathway

Epimedii Herba: An ancient Chinese herbal medicine in the prevention and treatment of rheumatoid arthritis

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