Magnified Fluorescent Aptasensors Based on a Gold Nanoparticle−DNA Hybrid and DNase I for the Cycling Detection of Mercury(II) Ions in Aqueous Solution

Abstract

Intake of water contaminated with mercury ions leads to various toxic effects and health issues. In this work, a novel gold nanoparticles (AuNPs) DNA hybrid sensor for fluorescent detection of mercury(II) ions (Hg 2+ ) in aqueous solution was prepared. The fluorescein was modified onto the terminal of ssDNA and it consequently presented a strong fluorescence. The probing behaviors toward various metal ions were investigated through the fluorescence intensity change. The result demonstrated that the amplification strategy based on ssDNA−AuNPs for Hg 2+ detection by using DNase I exhibited high sensitivity and selectivity. Under optimum condition, the resultant chemosensor tested the concentration of mercury ions in a linear range from 10 to 300 nM (R 2 = 0.9637), and a detection limit as low as 2.11 nM. Moreover, the developed sensor was used to detect Hg 2+ ions in the real samples, and the observed results were in good coordination with the value of calculation.

A Microfluidic Aptamer-Based Sensor for Detection of Mercury(II) and Lead(II) Ions in Water

“…During the past few years, many methods using different instruments have been reported for mercury and lead ion detection, including inductively coupled plasma mass spectrometry (ICP-MS) [5,6], cold-vapor atomic fluorescence spectroscopy (CV-AFS) [7], high-performance liquid chromatography (HPLC) [8], colorimetric sensors [9], magnetic beads [10], nanopore sensors [11,12], surface-enhanced Raman scattering (SERS) [13], electrochemical sensors [14,15] and aptasensors [16,17]. Although these methods provide reliable detection for mercury and lead ions, the mentioned methods suffer from some limitations.…”

High Catalytic Activity of Fluorophore-Labeled Y-Shaped DNAzyme/3D MOF-MoS2NBs as a Versatile Biosensing Platform for the Simultaneous Detection of Hg2+, Ni2+, and Ag+ Ions

“…In particular, the fluorescent sensor exhibits convenient advantages such as metal ion detection with remarkable superiority, sensitivity, low cost, easy operation, and efficiency. The fluorescent sensors have attracted extensive attention. , A number of fluorescent biosensors for heavy metal detection are reported over the past several years. , However, sensitive and selective fluorescent sensors for multiple detections of Hg 2+ , Ni 2+ , and Ag + ions are scarce. Frequently, significant practical limitations of Hg 2+ , Ni 2+ , and Ag + ions are seen, which include detailed synthesis schemes, high-cost methods, and insufficient selectivity.…”

“…The fluorescent sensors have attracted extensive attention. 12,13 A number of fluorescent biosensors for heavy metal detection are reported over the past several years. 14,15 However, sensitive and selective fluorescent sensors for multiple detections of Hg 2+ , Ni 2+ , and Ag + ions are scarce.…”

Dual-Emission Fluorescence Probe Based on CdTe Quantum Dots and Rhodamine B for Visual Detection of Mercury and Its Logic Gate Behavior

“…Toward this goal, in recent years, much effort has been focused on developing novel fluorescent detection systems for mercury, including sensors based on gold nanoparticles, organic dyes, quantum dots (QDs) and lanthanide-doped nanoparticles, etc. [ 10 , 11 , 12 , 13 , 14 ]. Among them, QDs fascinate researchers and reveal promising prospects in the fluorescence detection field due to their distinctive photophysical properties, such as high quantum efficiency, size-dependent photoluminescence, broad excitation window, high extinction coefficients and tunable emission spectra [ 13 ].…”