Magnetolithographic Patterning of Inner Walls of a Tube: A New Dimension in Microfluidics and Sequential Microreactors

Abstract

By applying magnetolithography it is possible to chemically pattern the inside of tubes. This new capability allows one to perform sequential processes within the tubes. Several enzymatic reactions are demonstrated.

Constructing 2D Nanosheet-Assembled MnCo2O4 Nanotubes for Pressure and Colorimetric Dual-Signal Readout Detection of Cancer Cells in Serum Samples

“…Moreover, MnCo 2 O 4 nanotubes show excellent peroxidase-like activity over a range of temperatures between 20 and 45 °C (Figure S12B). Compared to the most reported catalysts, − the reaction conditions of catalytic oxidation of TMB by the MnCo 2 O 4 catalyst in this work were not limited, which is essential for practical applications . In this work, the temperature and pH value of the MnCo 2 O 4 catalyst that we choose are 35 °C and 5.0, respectively.…”

Glucose level determination with a multi-enzymatic cascade reaction in a functionalized glass chip

“…The advantage of this Lab-on-a-Chip approach is that the functionalization procedure does not require additional steps for spatial separation of the enzymes such as patterning or implementation of valves. [34][35][36][37][38][39][40][41] Moreover, the small volume of the chips results in fast analysis times (<20 s). It is remarkable to note that the co-immobilization of GOx and HRP in microuidic channels does not affect their functionality: the enzymes do not show a loss of activity nor leaching during the glucose determination, are (very) stable in time and reusable.…”

“…Until now, most reported enzyme-functionalization procedures for Lab-ona-Chip devices for enzymatic cascade reactions for detection of glucose (or others biomarkers) have been based on separation of microuidic channels in various compartments by integration of valves, [34][35][36] or on patterning of a capillary into different enzymatic zones by means of shadow-mask lithography. [37][38][39][40][41] In this study we demonstrate co-immobilization of the enzymes GOx and HRP as a much simpler way to achieve a glucose sensor: GOx and HRP are randomly co-immobilized on the interior of a microuidic channel network of glass chips using poly(2-hydroethyl methacrylate) (PHEMA) polymer brushes as anchors. 42,43 Polymer brushes 44 have been used to immobilize enzymes or other catalysts on the wall of micro-uidic systems.…”

Sequential patterning of two fluorescent streptavidins assisted by photoactivatable biotin on an aminodextran-coated surface

“…The APTMS-silanized surface was immersed in a DMF solution of 1 M succinic anhydride for 4 h at 37 • C to change the amine functional group to the carboxylate surface, rinsed with distilled water, and dried under a stream of nitrogen. The carboxylate surface was activated with a solution of 0.1 M EDC and 0.025 M NHS in distilled water for 15 min, and immersed in 1 mg mL −1 aminodextran in 10 mM phosphate buffer (pH 7.5) to form an amine surface [18,19]. Finally, the surface was blocked with 1 M ethanolamine (pH 8.5), washed with deionized water, and dried under a stream of nitrogen.…”