Magneto-active substrates for local mechanical stimulation of living cells

Bidan, Cécile M.; Fratzl, Mario; Coullomb, Alexis; Moreau, Philippe; Lombard, Alain H.; Wang, Irène; Balland, Martial; Boudou, Thomas; Dempsey, Nora; Devillers, Thibaut; Dupont, Aurélie

doi:10.1101/204586

Cited by 6 publications

(5 citation statements)

References 45 publications

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“…It could be shown that both the height of the pillars and the interpillar distance are important parameters to harmonize stability, actuatability, and cellular morphology within the whole cell analysis microsystem. It is convincing that this platform provides an advantageous and well‐defined way to study the mechanical stimulation of different cell entities on the single cell level 41. As a proof of concept, the early molecular responses of hMSCs to the external, magnetically driven mechanical stimuli were analyzed.…”

Section: Resultsmentioning

confidence: 99%

PnBA/PDMAA‐Based Iron‐Loaded Micropillars Allow for Discrete Cell Adhesion and Analysis of Actuation‐Related Molecular Responses

Kojima

Husari

Dieterle

et al. 2020

Adv Materials Inter

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A new approach to generate magneto‐activatable microstructures for discrete cell adhesion to investigate cell mechanoresponsiveness is reported here. The system is based on poly(n‐butylacrylate) micropillar arrays, generated from prepolymers via C,H insertion chemistry and filled with magnetic iron oxide nanoparticles. The pillars' surfaces are modified such that only top faces of the pillars are cell attractive, while the pillar side walls and the area between the pillars are covered by a cell repelling hydrogel layer based on poly(N,N‐dimethyl acrylamide). Magnet stimulation leads to pillar deflection, which induces traction forces to adherent cells. Analysis of early mechanoresponse in human mesenchymal stem cells, specifically the localization of autophosphorylated focal adhesion kinase (pFAK Y397) and the co‐transcriptional activator yes‐associated protein (YAP), shows a clear redistribution of pFAK Y397 to the cell margins and an enhanced nuclear localization of YAP, following the magnetically induced mechanical actuation. The successful use of this technique shows that the generated magnetoactive posts are suitable tools to detect the onset (FAK) and maintenance (YAP) involved in stem cell mechanosensing and mechanotransduction. The platform combines selective cell adhesion with the possibility of magnetic actuation, thereby representing a promising technology to broaden the molecular understanding of cellular mechanobiology.

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Section: Resultsmentioning

confidence: 99%

PnBA/PDMAA‐Based Iron‐Loaded Micropillars Allow for Discrete Cell Adhesion and Analysis of Actuation‐Related Molecular Responses

Kojima

Husari

Dieterle

et al. 2020

Adv Materials Inter

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“…[ 5 ] Such materials have potential uses in soft robotics and wearable technology, [ 6,7 ] and in drug delivery and other biological actuation applications due to the remote nature of magnetic fields. [ 8,9 ]…”

Section: Figurementioning

confidence: 99%

Remote Sensing and Remote Actuation via Silicone–Magnetic Nanorod Composites

Stottlemire

Miller

Whitlow

et al. 2021

Adv Materials Technologies

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The capacity for a soft material to combine remote sensing and remote actuation is highly desirable for many applications in soft robotics and wearable technologies. This work presents a silicone elastomer with a suspension of a small weight fraction of ferromagnetic nickel nanorods, which is capable of both sensing deformation and altering stiffness in the presence of an external magnetic field. Cylinders composed of silicone elastomer and 1% by weight nickel nanorods experience large increases in compressive modulus when exposed to an external magnetic field. Incremental compressions totaling 600 g of force applied to the same silicone–nanorod composites increase the magnetic field strength measured by a Hall effect sensor enabling the material to be used as a soft load cell capable of detecting the rate, duration, and magnitude of force applied. In addition, lattice structures are 3D printed using an ink composed of silicone elastomer and 1% by weight nickel nanorods, which possess the same sensing capacity.

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“…[ 2 ] Numerous studies have focused on replicating these morphologies and exploring artificial cilia for applications in microfluidic propulsion/mixing, [ 3–7 ] surface property modification, [ 8 ] enhancing catalytic reactions, [ 9,10 ] sensing, [ 11–14 ] microrobots/swimmers, [ 15,16 ] object transportation, [ 17–19 ] and cell stimulation. [ 20 ]…”

Section: Figurementioning

confidence: 99%

Optomechanically Actuated Microcilia for Locally Reconfigurable Surfaces

Kim

Guidetti

et al. 2020

Advanced Materials

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torque that result in large material deformation. The spatial distribution of magnetic fields makes it challenging to obtain spatially selective actuation. Previous reports have shown localized control of cilia by controlling multiple permanent magnets independently [23] or by fabricating materials with embedded magnetic domains with different magnetization directions. [24,25] However, remote and precise local actuation remains challenging for magnetic microcilia structures. Light, by contrast, propagates long distances, wirelessly, without dramatic distortion, and, more importantly, using light allows for local stimulation with both high spatial and high temporal resolution. In

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Magneto-active substrates for local mechanical stimulation of living cells

Abstract: 18Cells are able to sense and react to their physical environment by translating a mechanical cue 19 into an intracellular biochemical signal that triggers biological and mechanical responses.

Cited by 6 publications

References 45 publications

PnBA/PDMAA‐Based Iron‐Loaded Micropillars Allow for Discrete Cell Adhesion and Analysis of Actuation‐Related Molecular Responses

PnBA/PDMAA‐Based Iron‐Loaded Micropillars Allow for Discrete Cell Adhesion and Analysis of Actuation‐Related Molecular Responses

Remote Sensing and Remote Actuation via Silicone–Magnetic Nanorod Composites

Optomechanically Actuated Microcilia for Locally Reconfigurable Surfaces

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