Magnetite nanoparticles−based hydroxyl radical scavenging activity assay of antioxidants using N, N-dimethyl-p-phenylenediamine probe

Abstract

Excessive amounts of reactive oxygen species (ROS), unless counterbalanced by antioxidants, can cause cellular damage under oxidative stress conditions; therefore, antioxidative defenses against ROS must be measured. With the development of nanotechnology, nanoparticles have found numerous applications in science, health, and industries. Magnetite nanoparticles (Fe3O4:MNPs) have attracted attention because of their peroxidase-like activity. In this study, hydroxyl radicals (•OH) generated by MNPs-catalyzed degradation of H2O2 converted the N,N-dimethyl-p-phenylenediamine (DMPD) probe into its colored DMPD•+ radical cation, which gave an absorbance maximum at λ = 553 nm. In the presence of antioxidants, •OH was partly scavenged by antioxidants and produced less DMPD•+, causing a decrease in the 553 nm-absorbance. Antioxidant concentrations were calculated with the aid of absorbance differences between the reference and sample solutions. The linear working ranges and trolox equivalent antioxidant capacity coefficients of different classes of antioxidants were determined by applying the developed method. In addition, binary and ternary mixtures of antioxidants were tested to observe the additivity of absorbances of mixture constituents. The method was applied to real samples such as orange juice and green tea. Student t-test, F tests, and the Spearman’s rank correlation coefficient were used for statistical comparisons.

Impact of Magnetite Nanoparticles Coated with Aspartic Acid on the Growth, Antioxidant Enzymes Activity and Chlorophyll Content of Maize

“…Recently, the development of biocompatible nanoparticles that have antioxidant properties has attracted a great deal of attention [10]. In addition, magnetite nanoparticles possess an intrinsic enzyme mimetic activity similar to natural peroxidase activity [11]. The peroxidase-like activity of magnetite nanoparticles is based on the coexistence of Fe 2+ /Fe 3+ ion pairing in the structure, such as Fenton's reagent, which are known to catalyze the breakdown of hydrogen peroxide [12].…”

Cobalt ferrite nanoparticle for the elimination of CD133+CD44+ and CD44+CD24−, in breast and skin cancer stem cells, using non-ionizing treatments

“…They use N,N-dimethyl- p -phenylenediamine (DMPD) probe (°OH detector) and F3O4 NPs with the size of 20–30 nm. They showed that in concentration around 0.1 mg/ml, absorbance of solution (F3O4 NP + DMPD) was around 0.6 [ 72 ]. While we sense among of °OH with Terephthalic acid + Core: citric acid: PEG: ICG: PpIX MNPs in presence of magnetic field and achieve high amount of °OH production in with combination of MNPs and magnetic field.…”