Magnetite Content Evaluation on Magnetic Drug Delivery Systems by Spectrophotometry: A Technical Note

Silva-Freitas, Érica Lira; Carvalho, José Ferreira de; Pontes, Thales Renan Ferreira; Araújo-Neto, Rafael P.; Carriço, A. S.; Egito, Eryvaldo Sócrates Tabosa do

doi:10.1208/s12249-011-9610-5

Cited by 8 publications

(5 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The content of magnetic iron oxide was determined using previously reported methods [ 13 ], with some modification. In brief, the magnetic iron oxide nanoparticles were separated using centrifugation after lyses of nanoliposomes.…”

Section: Methodsmentioning

confidence: 99%

Preparation, characterization and in vitro efficacy of magnetic nanoliposomes containing the artemisinin and transferrin

et al. 2014

View full text Add to dashboard Cite

BackgroundArtemisinin is the major sesquiterpene lactones in sweet wormwood (Artemisia annua L.), and its combination with transferrin exhibits versatile anti-cancer activities. Their non-selective targeting for cancer cells, however, limits their application. The aim of this study was to prepare the artemisinin and transferrin-loaded magnetic nanoliposomes in thermosensitive and non-thermosensitive forms and evaluate their antiproliferative activity against MCF-7 and MDA-MB-231 cells for better tumor-targeted therapy.MethodsArtemisinin and transferrin-loaded magnetic nanoliposomes was prepared by extrusion method using various concentrations of lipids. These formulations were characterized for particle size, zeta potential, polydispersity index and shape morphology. The artemisinin and transferrin-loading efficiencies were determined using HPLC. The content of magnetic iron oxide in the nanoliposomes was analysed by spectrophotometry. The in vitro release of artemisinin, transferrin and magnetic iron oxide from vesicles was assessed by keeping of the nanoliposomes at 37°C for 12 h. The in vitro cytotoxicity of prepared nanoliposomes was investigated against MCF-7 and MDA-MB-231 cells using MTT assay.ResultsThe entrapment efficiencies of artemisinin, transferrin and magnetic iron oxide in the non-thermosensitive nanoliposomes were 89.11% ± 0.23, 85.09% ± 0.31 and 78.10% ± 0.24, respectively. Moreover, the thermosensitive formulation showed a suitable condition for thermal drug release at 42°C and exhibited high antiproliferative activity against MCF-7 and MDA-MB-231 cells in the presence of a magnetic field.ConclusionsOur results showed that the thermosensitive artemisinin and transferrin-loaded magnetic nanoliposomes would be an effective choice for tumor-targeted therapy, due to its suitable stability and high effectiveness.

show abstract

Section: Methodsmentioning

confidence: 99%

Preparation, characterization and in vitro efficacy of magnetic nanoliposomes containing the artemisinin and transferrin

et al. 2014

View full text Add to dashboard Cite

show abstract

“…As reported previously, the magnetic properties and morphology of prepared nanoliposomes were analyzed by vibrating sample magnetometer (Meghnatis Daghigh Kavir Co., Iran) and cryo-transmission electron microscopy (cryo-TEM), respectively. [ 12 13 ]…”

Section: Methodsmentioning

confidence: 99%

Experimental treatment of breast cancer-bearing BALB/c mice by artemisinin and transferrin-loaded magnetic nanoliposomes

Ghorbanihaghjo¹,

Faezizadeh²,

Mesbah-Namin³

et al. 2015

Phcog Mag

View full text Add to dashboard Cite

Background:The combination of artemisinin and transferrin exhibits versatile anticancer activities. In previous, we successfully prepared artemisinin and transferrin-loaded magnetic nanoliposomes and evaluated their anti-proliferative activity against MCF-7 and MDA-MB-231 cell lines in vitro. In this study, we investigate the in vivo anti-breast cancer activity of artemisinin and transferrin-loaded magnetic nanoliposome against breast transplanted tumors in BALB/c mice model.Materials and Methods:Artemisinin and transferrin-loaded magnetic nanoliposomes were prepared and characterized for some physiochemical properties. Pieces of tumor tissue from the breast cancer-bearing BALB/c mice were transplanted subcutaneously to the syngeneic female BALB/c mice. In the presence of the external magnet that placed at the breast tumor site, the tissue distribution and tumor-suppressing effects of prepared nanoliposomes on tumor growth was evaluated.Results:The prepared nanoliposomes have fine spherical shape, rough surface, nano-sized diameter and magnetic properties. At 2 h after treatment, the intravenous administration of artemisinin and transferrin-loaded magnetic nanoliposomes followed using the magnetic field approximately produced 10- and 5.5-fold higher levels of artemisinin and transferrin in the tumors, respectively, compared with free artemisinin and transferrin. Moreover, in the presence of an external magnetic field, the prepared nanoliposomes could significantly induce apoptosis in the mice breast cancer cells as well as could reduce tumor volume in tumorized mice at 15 days after treatment.Conclusion:The data suggested that the artemisinin and transferrin-loaded magnetic nanoliposomes would be a good choice for the breast tumor-targeted therapy, due to its high targeting efficiency.

show abstract

“…The use of the magnetic force to delay the transit of orally administered drugs may become an attractive strategy for enhancing the efficacy of orally delivered systems. Despite the promising properties, magnetic particles may dissolve in acid media (Silva-Freitas et al, 2011). Such possible particle loss could reduce the efficiency of the magnetic system used as a drug carrier.…”

Section: Oral Administrationmentioning

confidence: 99%

Advances in Applied Biotechnology

L¹

2015

Lecture Notes in Electrical Engineering

View full text Add to dashboard Cite

in 1981. He worked as a microbiologist at the National Institute of R&D in Floriculture, as a research assistant, and as a principal investigator at R&D Institute for Vegetables and Fruits Processing. He has also been acting as a lecturer at the Ecological University of Bucharest and worked as a senior researcher at the National Institute for R&D in Horticultural Biotechnology Stefanesti-Arges. Since 2007, he has been teaching as a professor of biotechnology for environmental protection, microbiology and bioremediation at the University of Pitesti, Faculty of Sciences. So far, he wrote and published over 140 papers, 50 of them being published in international journals and proceeding volumes all over the world; he published 10 books in Romania and had 2 book chapters published by Marcel Dekker and Kluwer Academic Publishers. During the last decade, he registered, as first author, 10 Romanian patents in the field of biotechnology for edible and medicinal mushroom cultivation and has been awarded with gold and silver medals at international exhibitions for inventions, research and new technologies. Contents Preface XI Part Biotechnology of Agricultural Wastes -Recycling, Saving Energy and Food Quality Preservation 1 Chapter Biotechnology of Agricultural Wastes Recycling Through Controlled Cultivation of Mushrooms 3

show abstract

Magnetite Content Evaluation on Magnetic Drug Delivery Systems by Spectrophotometry: A Technical Note

Cited by 8 publications

References 22 publications

Preparation, characterization and in vitro efficacy of magnetic nanoliposomes containing the artemisinin and transferrin

Preparation, characterization and in vitro efficacy of magnetic nanoliposomes containing the artemisinin and transferrin

Experimental treatment of breast cancer-bearing BALB/c mice by artemisinin and transferrin-loaded magnetic nanoliposomes

Advances in Applied Biotechnology

Contact Info

Product

Resources

About