Magnetically Stimulated Drug Release Using Nanoparticles Capped by Self-Assembling Peptides

Abstract

A novel self-assembling peptide-functionalized core–shell mesoporous silica nanoparticle was developed as a drug carrier. Superparamagnetic manganese- and cobalt-doped iron oxide nanoparticles formed the core for the mesoporous silica shell coating. On the silica outer shell, the peptide Boc–Phe–Phe–Gly–Gly–COOH was covalently conjugated by 1-ethyl-3-(3-dimethylaminopropyl)­carbodiimide hydrochloride and N-hydroxysulfosuccinimide sodium salt coupling. The self-assembling property of the peptide at physiological temperature was utilized to block the pore openings, while the disassembly at elevated local particle temperature released cargo molecules without bulk heating that would cause cell damage. Both conventional heating and heating in an alternating magnetic field were tested for the release of fluorescein and daunorubicin. In vitro experiments showed high cytotoxicity on pancreatic carcinoma cells (PANC-1) when this delivery system was activated by an alternating magnetic field, while control particles without drugs showed no obvious cytotoxicity.

The Peptide Functionalized Inorganic Nanoparticles for Cancer-Related Bioanalytical and Biomedical Applications

“…The peptide functionalized NPs have been extensively employed to avoid non-specific delivery-related issues in the diagnosis and therapy of cancer. Especially, the role of CPP functionalized NPs has been explored in the areas, such as molecular imaging and anticancer drug delivery, because CPPs aid in cellular internalization of various NPs without damaging the cell, and the CPP functionalized NPs can be served as cargos for intracellular transport of various species including radioactive isotopes [ 173 , 208 , 209 , 210 ], small molecules [ 208 , 211 , 212 , 213 , 214 , 215 , 216 , 217 , 218 , 219 , 220 , 221 , 222 , 223 , 224 , 225 , 226 , 227 , 228 , 229 , 230 , 231 ], oligonucleotides [ 232 , 233 , 234 , 235 , 236 , 237 , 238 , 239 , 240 , 241 , 242 , 243 , 244 , 245 , 246 , 247 , 248 , 249 , 250 ,…”

Engineering Oxygen‐Irrelevant Radical Nanogenerator for Hypoxia‐Independent Magnetothermodynamic Tumor Nanotherapy

“…[ 32 ] Alternatively, the integration of noninvasive alternating magnetic fields (AMF) with superparamagnetic nanoparticles may provide an alternative approach to conquer this critical issue. [ 33–35 ] On the one hand, no penetration depth limitation facilitates that the AMF actuation can be triggered in the deep‐seated tumor tissues without concern of absorption by tissues or light scattering. [ 30,31 ] On the other hand, free‐radical generation can be spatio‐temporally controlled by magnetic hyperthermia effect, [ 36,37 ] offering the merits for delivering high level of free radicals to the diseased sites and lowering side effects on normal tissues.…”

Stimulus-Responsive Nanomedicines for Disease Diagnosis and Treatment

“…This novel class of Mesoporous silica nanoparticles (MSNs) could maintain stability and safety under normal environment in human body. Once the magnetic core is triggered by magnetic field, the heat generated can lead to the change of its structure, giving rise to the release of drugs [ 146 ] ( Figure 7 ).…”

“…The heat caused the decomposition of the peptide, leading to the release of the cargo from the pores of the MSN. Reproduced with permission from the publishers of corresponding reference [ 146 ].…”