Magnetically-separable and highly-stable enzyme system based on crosslinked enzyme aggregates shipped in magnetite-coated mesoporous silica

Lee, Jun Young; Na, Hyon Bin; Kim, Byoung Chan; Lee, Jin Hyung; Lee, Byoung-Soo; Kwak, Ja Hun; Hwang, Yosun; Park, Je‐Geun; Gu, Man Bock; Kim, Jaeyun; Joo, Jin; Shin, Chae Ho; Grate, Jay W.; Hyeon, Taeghwan; Kim, Jungbae

doi:10.1039/b909109b

Cited by 41 publications

(30 citation statements)

References 43 publications

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“…It was reported that when α-chymotrypsin (CT) was immobilized in magnetic hierarchically-ordered mesocellular mesoporous silica (HMMS) by adsorption of enzymes and subsequent enzyme crosslinking (CLEA-M-CT), the resulting CLEA-M-CT could maintain their initial activity not only under shaking (250 rpm) for 30 days, but also under being recycled for 35 times [20]. Magnetic cross-linked enzyme aggregates of α-amylase were also prepared by chemical cross-linking the enzyme aggregates with amino functionalized magnetite nanoparticles.…”

Section: Introductionmentioning

confidence: 99%

Hybrid Magnetic Cross-Linked Enzyme Aggregates of Phenylalanine Ammonia Lyase from Rhodotorula glutinis

Cui

Zhang

et al. 2014

PLoS ONE

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Novel hybrid magnetic cross-linked enzyme aggregates of phenylalanine ammonia lyase (HM-PAL-CLEAs) were developed by co-aggregation of enzyme aggregates with magnetite nanoparticles and subsequent crosslinking with glutaraldehyde. The HM-PAL-CLEAs can be easily separated from the reaction mixture by using an external magnetic field. Analysis by scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM) indicated that PAL-CLEAs were inlayed in nanoparticle aggregates. The HM-PAL-CLEAs revealed a broader limit in optimal pH compared to free enzyme and PAL-CLEAs. Although there is no big difference in Km of enzyme in CLEAs and HM-PAL-CLEAs, Vmax of HM-PAL-CLEAs is about 1.75 times higher than that of CLEAs. Compared with free enzyme and PAL-CLEAs, the HM-PAL-CLEAs also exhibited the highest thermal stability, denaturant stability and storage stability. The HM-PAL-CLEAs retained 30% initial activity even after 11 cycles of reuse, whereas PAL-CLEAs retained 35% of its initial activity only after 7 cycles. These results indicated that hybrid magnetic CLEAs technology might be used as a feasible and efficient solution for improving properties of immobilized enzyme in industrial application.

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Section: Introductionmentioning

confidence: 99%

Hybrid Magnetic Cross-Linked Enzyme Aggregates of Phenylalanine Ammonia Lyase from Rhodotorula glutinis

Cui

Zhang

et al. 2014

PLoS ONE

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“…Strategic procedure, so called 'ship-in-a-bottle' was employed by Lee et al (2009) to develop the CLEA of a-chymotrypsin in superparamagnetic hierarchically ordered mesocellular mesoporous silica (M-HMMS) (Fig. 6).…”

Section: Enzyme Crosslinkingmentioning

confidence: 99%

Immobilization of biocatalysts for enzymatic polymerizations: Possibilities, advantages, applications

Miletić¹,

Nastasović

Loos

2012

Bioresource Technology

178

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a b s t r a c tBiotechnology also holds tremendous opportunities for realizing functional polymeric materials. Biocatalytic pathways to polymeric materials are an emerging research area with not only enormous scientific and technological promise, but also a tremendous impact on environmental issues. Many of the enzymatic polymerizations reported proceed in organic solvents. However, enzymes mostly show none of their profound characteristics in organic solvents and can easily denature under industrial conditions. Therefore, natural enzymes seldom have the features adequate to be used as industrial catalysts in organic synthesis. The productivity of enzymatic processes is often low due to substrate and/or product inhibition. An important route to improving enzyme performance in non-natural environments is to immobilize them.In this review we will first summarize some of the most prominent examples of enzymatic polymerizations and will subsequently review the most important immobilization routes that are used for the immobilization of biocatalysts relevant to the field of enzymatic polymerizations.

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“…39 However, magnetic mesoporous silica particles have been developed to facilitate an easy separation of the immobilized enzymes from products. 40,41 Nanofibers. Polymer nanofibers have continuously attracted attention for largescale operations and applications due to their unique properties, which allow researchers to achieve a high enzyme loading and enzyme catalytic efficiencies.…”

Section: Synthesis Of Nanomaterials For Enzyme Immobilizationmentioning

confidence: 99%

Nanomaterial‐Based Biocatalyst

Lee

et al. 2013

Nanocatalysis Synthesis and Applications

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Magnetically-separable and highly-stable enzyme system based on crosslinked enzyme aggregates shipped in magnetite-coated mesoporous silica

Cited by 41 publications

References 43 publications

Hybrid Magnetic Cross-Linked Enzyme Aggregates of Phenylalanine Ammonia Lyase from Rhodotorula glutinis

Hybrid Magnetic Cross-Linked Enzyme Aggregates of Phenylalanine Ammonia Lyase from Rhodotorula glutinis

Immobilization of biocatalysts for enzymatic polymerizations: Possibilities, advantages, applications

Nanomaterial‐Based Biocatalyst

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