Magnetically sensitive nanocomplex enhances antitumor efficacy of dendritic cell-based immunotherapy

Abstract

Summary. Background: One of the major factors restricting in vivo efficacy of dendritic cells (DCs) based immunotherapy is the inefficient migration of these cells to the lymphoid tissue, wherein DCs activate antigen-specific T cells. A fundamentally new approach for the possibility of enhancing the antitumor effects of DC-based immunotherapy may be the use of magnetically sensitive nanocomplexes to increase the target delivery of DCs to the lymph nodes of the recipient. Aim: To study the antitumor and immunomodulatory effects of the DC-nanovaccine with magnetosensitive properties and its influence on the immunosuppressive tumor microenvironment in mice with sarcoma 37. Materials and Methods: The antitumor, antimetastatic and immunomodulatory effects of DCs loaded with magnetic nanocomplex under magnetic field (MF) control in mice with sarcoma 37 have been investigated. Results: Combined therapy contributed to a significant reduction in tumor volume and weight compared to the control group of mice and mice that received the DC vaccine without MF. Therapy with magnetically sensitive DC nanovaccine with and without the addition of the MF was accompanied by a significant down-regulation of the level of FoxP3, transforming growth factor β, interleukin (IL)-10 and vascular endothelial growth factors, mRNA expression in tumor tissues. A significant increase in interferon-γ and IL-4 mRNA expression was found in mice treated with the magnetically sensitive DC nanovaccine under MF control. Conclusion: A significant increase in the antitumor efficacy of the DC vaccine can be achieved using magnetosensitive nanocarriers of tumor antigens under MF control.

Tumor Microenvironment Regulation and Cancer Targeting Therapy Based on Nanoparticles

“…Nanomaterials are widely employed for anti-tumor treatment and TME regulation [ 21 , 22 , 23 ]. The nanomaterials with modulators such as multifunctional platforms can effectively eliminate the primary cancer, inhibit the distal metastasis, and prevent cancer recurrence [ 22 , 24 , 25 , 26 ]. Nanomaterials mainly regulate TME through the following four mechanisms: (i).…”