Magnetically Sensitive Alginate-Templated Polyelectrolyte Multilayer Microcapsules for Controlled Release of Doxorubicin

Abstract

Magnetically sensitive alginate-templated polyelectrolyte multilayer microcapsules were successfully synthesized by a novel process combining emulsification and layer-by-layer self-assembly techniques. The as-synthesized microcapsules (2.67 µm in diameter) were superparamagnetic with a saturation magnetization of 14.2 emu • g -1 , which contained approximately 30 wt % maghemite nanoparticles. The drug (doxorubicin) encapsulation efficiency was 56.4% and loading content was 3.5%. The in vitro release behavior under a high-frequency magnetic field (HFMF) indicated that the applied HFMF accelerated significantly the drug release from the microcapsules, which might be related to the microcapsules' magnetic properties. Moreover, the in vitro cytotoxicity of doxorubicin-loaded alginate-templated polyelectrolyte multilayer microcapsules and the doxorubicin released from the microcapsules were investigated.

Influence of charge on encapsulation and release behavior of small molecules in self-assembled layer-by-layer microcapsules

“…These were in DOI: 10.3109/10717544.2013.867381 correlation with the studies performed with similar polymers and other charged polymers (Mak et al, 2008). LbL-MC have been studied for encapsulation efficiency of small model molecules, including 5-FU (Yan et al, 2011), perylene (Manna & Patil, 2009), curcumin (Manju & Sreenivasan, 2011) ciprofloxacin hydrochloride (Mao et al, 2005), doxorubicin (Tao et al, 2007;Liu et al, 2010) and daunorubicin (Han et al, 2008). In this study, we chose small molecules with different charge.…”

Magnetic alginate microspheres detected by MRI fabricated using microfluidic technique and release behavior of encapsulated dual drugs

“…Trisodium citratestabled SPIO NPs were prepared by a method described elsewhere. 29,30 The diameter of the obtained SPIO NPs was ~10 nm measured from the image of transmission electron microscopy (TEM). The SPIO NPs were re-dispersed in water at a concentration of 10 mg/mL as a stock solution for further use.…”

“…It may be ascribed to a strong electrostatic interaction between DOX⋅HCl with positive charge and carboxyl groups of Ca-ALG with negative charge. 30 In contrast, there is only hydrogen bond between 5-Fu and alginate with relatively weak interaction force. The interaction between the two drugs and alginate could be deduced from their chemical structure as shown in Figure S4.…”

Bioactive microsphere-based coating for biomedical-textiles with encapsulated antimicrobial peptides (AMPs)

“…This process was repeated (2-6 layers) using the oppositely charged polyelectrolyte until expected multilayer pattern was obtained, where chitosan was used as polycation and alginate as polyanion. The layer sequence was (Alg/CS)n. as described by Liu and co-workers and illustrated in figure 1 [11]. …”

“…Moreover, various bioactive compounds can be sequestered into the capsule for drug delivery, sensing, or catalysis applications, and capsule surface can be modified to alter the functionality and/or improve the colloidal stability of the capsules [9,10]. The polyelectrolyte multilayer coatings may serve multiple purposes such as stabilizing alginate hydrogels against dissolution in biological environments and providing barrier membranes for alginate hydrogels to slow release of encapsulated drug or biomolecules [11]. On the other hand, many of the antimicrobials used as finishing agents for textile materials are toxic which means that there is a growing public concern about the possible effects of antimicrobial finishes in environmental and biological systems.…”