Magnetically immobilized edible Bacillus natto for the biotransformation of polydatin to resveratrol and its bioactivity assessment

Wood-inspired high-efficiency bioreactors with hierarchical porous structures for continuous flow reactions

“…41 In our previous studies, β-glucosidase was found to show good effects in hydrolyzing polydatin to produce resveratrol. 42,43 Therefore, in this study, β-glucosidase was used for affinity immobilization to catalyze the hydrolysis of polydatin. Before immobilization, a polyhistidine sequence was inserted into the DNA sequence of β-glucosidase (2647 bp, expressed molecular weight of 95 kDa) to express histidine-tagged β-glucosidase by GS115 Pichia pastoris (the result from DNA electrophoresis is shown in Fig.…”

“…In addition, this bioreactor loads the enzyme by affinity immobilization, eliminating the complex step of enzyme purification and greatly reducing the cost of the whole process from enzyme production to the construction of the bioreactor. Although the conversion rate achieved by immobilized microorganisms also reaches 96%, 43 this takes 48 hours, and due to the metabolism of microorganisms, many impurities will be produced, which leads to difficulties in the subsequent separation of resveratrol. In contrast, the CBR not only uses its 3D hierarchical structure to achieve stable and efficient catalysis of enzymes but it also involves simple operation and low cost, which greatly simplifies the industrial upstream and downstream production process, showing great application potential.…”

Antioxidant Activity and Mechanism of Resveratrol and Polydatin Isolated from Mulberry (Morus alba L.)

“…Moreover, in nature, PD tends to be more abundant than RES [13]. Therefore, there are many studies devoted to converting PD into RES, but ignore the functional properties of PD compounds [14]. It is worth noting that PD also performs well in many biological functions, such as anti-atherosclerosis [15], improving heart function [16], reducing cholesterol [17], and protecting against liver injury [18].…”