Magnetically guided virus stamping for the targeted infection of single cells or groups of cells

Schubert, Rajib; Herzog, Sophie; Trenholm, Stuart; Roska, Botond; Müller, Daniel J.

doi:10.1038/s41596-019-0221-z

Cited by 12 publications

(11 citation statements)

References 51 publications

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“…In summary, our study supports the notion that, unlike sensory thalamic loops like MGB →A1 , associative thalamic loops like the MD →PL are composed of different input-output microcircuits residing within the same nucleus. In the future, mapping the diversity of microcircuits within cognitive thalamic nuclei, like the MD, using techniques such as high-resolution single-cell terminal mapping ( Lichtman et al, 2008 ; Peng et al, 2020 ) or monosynaptic retrograde tracings from single thalamic neurons ( Ghanem and Conzelmann, 2016 ; Schubert et al, 2019 ) will be necessary. The circuit motifs so revealed will help inform and generate computational models of the role of the cognitive thalamus, beyond that of a relay, in generating behavioral flexibility.…”

Section: Discussionmentioning

confidence: 99%

Variation of connectivity across exemplar sensory and associative thalamocortical loops in the mouse

Mukherjee

Bajwa

Lam

et al. 2020

eLife

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The thalamus engages in sensation, action, and cognition, but the structure underlying these functions is poorly understood. Thalamic innervation of associative cortex targets several interneuron types, modulating dynamics and influencing plasticity. Is this structure-function relationship distinct from that of sensory thalamocortical systems? Here, we systematically compared function and structure across a sensory and an associative thalamocortical loop in the mouse. Enhancing excitability of mediodorsal thalamus, an associative structure, resulted in prefrontal activity dominated by inhibition. Equivalent enhancement of medial geniculate excitability robustly drove auditory cortical excitation. Structurally, geniculate axons innervated excitatory cortical targets in a preferential manner and with larger synaptic terminals, providing a putative explanation for functional divergence. The two thalamic circuits also had distinct input patterns, with mediodorsal thalamus receiving innervation from a diverse set of cortical areas. Altogether, our findings contribute to the emerging view of functional diversity across thalamic microcircuits and its structural basis.

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Section: Discussionmentioning

confidence: 99%

Variation of connectivity across exemplar sensory and associative thalamocortical loops in the mouse

Mukherjee

Bajwa

Lam

et al. 2020

eLife

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“…[86][87][88] For example, introduction of unnatural amino acid residues such as azides on the capsid surface can give access to selective click chemistry. 89,90 Further, genetically modified viruses can enhance TE, 91,92 broaden the tropism, 63,93 enable fluorescent imaging, 85 escape neutralizing antibodies, 94,95 generate stimuli responsive vectors, 96 e.g. by light 97,98 or enzymes, 99 and target cells.…”

Section: Physical Methodsmentioning

confidence: 99%

Materials promoting viral gene delivery

Kaygisiz

Synatschke

2020

Biomater. Sci.

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“…For example, studies evidenced that a concentration range of 10 ng to 30 pg of triple-labeled Tat peptide-derivatized MNMPs per cell had no cytotoxic effect . As another example, a homogeneous distribution of MNMPs under the control of magnetic fields resulted in an enhanced delivery dose of MNMPs loaded with recombinant adeno-associated virus (rAAV). , Likewise, an increased transfection efficiency of programmable RNA-loaded PEI MNMPs was successfully evidenced …”

Section: Interaction Of Biological Specimens With Mnmpsmentioning

confidence: 98%

Recent Advancements of Specific Functionalized Surfaces of Magnetic Nano- and Microparticles as a Theranostics Source in Biomedicine

Zheng

Schumacher

et al. 2021

ACS Biomater. Sci. Eng.

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Magnetic nano-and microparticles (MNMPs) belong to a highly versatile class of colloids with actuator and sensor properties that have been broadly studied for their application in theranostics such as molecular imaging and drug delivery. The use of advanced biocompatible, biodegradable polymers and polyelectrolytes as MNMP coating materials is essential to ensure the stability of MNMPs and enable efficient drug release while at the same time preventing cytotoxic effects. In the past years, huge progress has been made in terms of the design of MNMPs. Especially, the understanding of coating formation with respect to control of drug loading and release kinetics on the molecular level has significantly advanced. In this review, recent advancements in the field of MNMP surface engineering and the applicability of MNMPs in research fields of medical imaging, diagnosis, and nanotherapeutics are presented and discussed. Furthermore, in this review the main emphasis is put on the manipulation of biological specimens and cell trafficking, for which MNMPs represent a favorable tool enabling transport processes of drugs through cell membranes. Finally, challenges and future perspectives for applications of MNMPs as theranostic nanomaterials are discussed.

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Magnetically guided virus stamping for the targeted infection of single cells or groups of cells

Cited by 12 publications

References 51 publications

Variation of connectivity across exemplar sensory and associative thalamocortical loops in the mouse

Variation of connectivity across exemplar sensory and associative thalamocortical loops in the mouse

Materials promoting viral gene delivery

Recent Advancements of Specific Functionalized Surfaces of Magnetic Nano- and Microparticles as a Theranostics Source in Biomedicine

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