Magnetically assisted delivery of cells using a magnetic resonance imaging system

Riegler, Johannes; Allain, Baptiste; Cook, Richard; Lythgoe, Mark F.; Pankhurst, Quentin A.

doi:10.1088/0022-3727/44/5/055001

Cited by 15 publications

(19 citation statements)

References 24 publications

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“…Whereas this technique has been used to not only enrich and detect circulating tumor cells in the blood stream but also delivery stem cells in injury site, its use for magnetic guided in-vivo delivery of immunological cells has been rarely reported so far. [17,18,20]…”

Section: Introductionmentioning

confidence: 99%

The manipulation of natural killer cells to target tumor sites using magnetic nanoparticles

et al. 2012

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The present work demonstrates that Cy5.5 conjugated Fe3O4/SiO2 core/shell nanoparticles could allow us to control movement of human natural killer cells (NK-92MI) by an external magnetic field. Required concentration of the nanoparticles for the cell manipulation is as low as ~20 μg Fe/mL. However, the relative ratio of the nanoparticles loaded NK-92MI cells infiltrated into the target tumor site is enhanced by 17-fold by applying magnetic field and their killing activity is still maintained as same as the NK-92MI cells without the nanoparticles. This approach allows us to open alternative clinical treatment with reduced toxicity of the nanoparticles and enhanced infiltration of immunology to the target site.

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Section: Introductionmentioning

confidence: 99%

The manipulation of natural killer cells to target tumor sites using magnetic nanoparticles

et al. 2012

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“…Over the past 15 years, magnetic cell therapy has gained interest as a modification for improving the delivery of therapeutic cells to a target region and prolonging their presence there 38 . Preclinical reports have stated that magnetic cell therapy has resulted in 1.5–30-fold greater cell delivery to a target region and a sustained cell population compared non-magnetic cell therapy 38 39 40 41 42 43 . Consequently, magnetic cell therapy might reduce the costs associated with cell production by requiring fewer cell injections, side effects such as off-targeting due to loss of cells, enhancing therapeutic effects.…”

Section: Discussionmentioning

confidence: 99%

Hybrid-Actuating Macrophage-Based Microrobots for Active Cancer Therapy

Han

Jin

Nguyen

et al. 2016

Sci Rep

102

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Using macrophage recruitment in tumors, we develop active, transportable, cancer theragnostic macrophage-based microrobots as vector to deliver therapeutic agents to tumor regions. The macrophage-based microrobots contain docetaxel (DTX)-loaded poly-lactic-co-glycolic-acid (PLGA) nanoparticles (NPs) for chemotherapy and Fe3O4 magnetic NPs (MNPs) for active targeting using an electromagnetic actuation (EMA) system. And, the macrophage-based microrobots are synthesized through the phagocytosis of the drug NPs and MNPs in the macrophages. The anticancer effects of the microrobots on tumor cell lines (CT-26 and 4T1) are evaluated in vitro by cytotoxic assay. In addition, the active tumor targeting by the EMA system and macrophage recruitment, and the chemotherapeutic effect of the microrobots are evaluated using three-dimensional (3D) tumor spheroids. The microrobots exhibited clear cytotoxicity toward tumor cells, with a low survivability rate (<50%). The 3D tumor spheroid assay showed that the microrobots demonstrated hybrid actuation through active tumor targeting by the EMA system and infiltration into the tumor spheroid by macrophage recruitment, resulting in tumor cell death caused by the delivered antitumor drug. Thus, the active, transportable, macrophage-based theragnostic microrobots can be considered to be biocompatible vectors for cancer therapy.

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“…Additionally cell aggregation due to dipole interactions at the magnetic flux density used is likely to increase targeting efficiency. 58 Nevertheless, we envisage the best delivery protocol would be to stop blood flow for 5 min, while cells are delivered via a catheter which is being retracted. This would increase the targeting efficiency to almost 100%.…”

Section: Discussionmentioning

confidence: 99%