Magnetic Ti₃C₂ MXene Nanosheets Prepared for Enrichment of Phosphopeptides

Abstract

MXenes have received lots of attention since discovered and have been applied in various fields. In this work, Ti 3 C 2 − Fe 3 O 4 composites with exposed non-modified Ti 3 C 2 MXene nanosheets were designed and prepared by an in situ growth strategy and then applied in the enrichment of phosphopeptides. The two-dimensional composites could interact with the phosphopeptides through a metal oxide affinity chromatography mechanism provided by Ti−O and Fe−O bonds and a hydrophilic interaction chromatography mechanism by surface hydroxyl groups. This magnetic nanomaterial with a specific surface area of 66.1 m 2 •g −1 had high sensitivity to phosphopeptides (0.5 nmol•L −1 ) and high selectivity (1:1000 of the molar ratio of β-casein to bovine serum albumin). Non-fat milk was adopted as a real sample to preliminarily examine the applicability of the Ti 3 C 2 −Fe 3 O 4 -based protocol. Subsequently, Qingkailing injection, a kind of traditional Chinese medicine injection, was introduced to further explore the suitability of the nanocomposites for phosphopeptide enrichment from more complex matrices and satisfactory results were obtained.

Multimodal Affinity-Modulated Efficient Separation of Lysozyme with a Hierarchical MXene@MOF Hybrid Framework

“…As summarized in Table S11, MX@MOF-DH-3 shows prominent binding performance over other adsorbents, including MOFs, molecularly imprinted polymers, functional macromolecules, magnetic hybrids, and derivatives. Most importantly, the adsorption of protein onto MX@MOF-DH-3 is much faster than that of recently reported MOF and MXene-based adsorbents, − attributed to the multivalent affinity and layered structure-powered rapid mass transport. These results further confirm the outstanding preponderance of the hierarchical MXene@MOF hybrid frameworks with multimodal adsorption affinity for the capture of proteins.…”

Effective Enrichment of Phosphopeptides Using Magnetic Polyoxometalate-Based Metal–Organic Frameworks

“…As displayed in Figure A,B,C, three, four, and six phosphopeptide peaks were enriched by Fe 3 O 4 , Fe 3 O 4 @SiO 2 , and Fe 3 O 4 @SiO 2 @Arg, respectively. In the mass spectrum of the sample after enrichment by Fe 3 O 4 -POMOFs, 13 phosphopeptides peaks were identified with clean background (Figure D) . The detected phosphopeptides from α-casein and β-casein are given in Table S1.…”

“…In the mass spectrum of the sample after enrichment by Fe 3 O 4 -POMOFs, 13 phosphopeptides peaks were identified with clean background (Figure 4D). 37 The detected phosphopeptides from α-casein and β-casein are given in Table S1.…”

Enrichment of Phosphopeptides Based on Zirconium Phthalocyanine-Modified Magnetic Nanoparticles

“…In terms of phosphopeptide enrichment, immobilized metal ion affinity chromatography (IMAC) is a normally used strategy. IMAC enrichment method has a strong affinity interaction between metal ions and phosphopeptides. , In this technique, metal ions are grafted onto substrate materials through chelators. However, the procedure for fixing metal ions is cumbersome and time-consuming.…”