Magnetic three-phase single-drop microextraction for highly sensitive detection of aflatoxin B1 in agricultural product samples based on peroxidase-like spatial network structure

Kuang, Jingyu; Ju, Jiahe; Li, Yongli; Chen, Yitong; Liu, Chang; Kong, Dezhao; Shen, Wei; Shi, Hang; Li, Li; Ye, Jin; Tang, Sheng

doi:10.1016/j.foodchem.2023.135856

Cited by 9 publications

(8 citation statements)

References 42 publications

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“…Reprinted with permission from Kuang et al. (2023). (c) Scheme of express competitive detection of ochratoxin A with the proposed lateral flow immunoassay based on magnetic biolabels.…”

Section: Roles and Applications Of Mnps In Mycotoxin Detectionmentioning

confidence: 99%

Magnetic nanoparticle‐based immunosensors and aptasensors for mycotoxin detection in foodstuffs: An update

Gao,

Zhou,

Zhang

et al. 2023

Comp Rev Food Sci Food Safe

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Mycotoxin contamination of food crops is a global challenge due to their unpredictable occurrence and severe adverse health effects on humans. Therefore, it is of great importance to develop effective tools to prevent the accumulation of mycotoxins through the food chain. The use of magnetic nanoparticle (MNP)‐assisted biosensors for detecting mycotoxin in complex foodstuffs has garnered great interest due to the significantly enhanced sensitivity and accuracy. Within such a context, this review includes the fundamentals and recent advances (2020–2023) in the area of mycotoxin monitoring in food matrices using MNP‐based aptasensors and immunosensors. In this review, we start by providing a comprehensive introduction to the design of immunosensors (natural antibody or nanobody, random or site‐oriented immobilization) and aptasensors (techniques for aptamer selection, characterization, and truncation). Meanwhile, special attention is paid to the multifunctionalities of MNPs (recoverable adsorbent, versatile carrier, and signal indicator) in preparing mycotoxin‐specific biosensors. Further, the contribution of MNPs to the multiplexing determination of various mycotoxins is summarized. Finally, challenges and future perspectives for the practical applications of MNP‐assisted biosensors are also discussed. The progress and updates of MNP‐based biosensors shown in this review are expected to offer readers valuable insights about the design of MNP‐based tools for the effective detection of mycotoxins in practical applications.

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“…Reprinted with permission from Kuang et al. (2023). (c) Scheme of express competitive detection of ochratoxin A with the proposed lateral flow immunoassay based on magnetic biolabels.…”

Section: Roles and Applications Of Mnps In Mycotoxin Detectionmentioning

confidence: 99%

Magnetic nanoparticle‐based immunosensors and aptasensors for mycotoxin detection in foodstuffs: An update

Gao,

Zhou,

Zhang

et al. 2023

Comp Rev Food Sci Food Safe

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“…It is known that a majority of artificial peroxidase-like nanozymes show more economical and robust catalytic activity than natural enzymes, which has exhibited great potential in developing efficient analytical techniques for food quality and safety analysis. − However, most of the peroxidase mimic-based assays usually required directly added H 2 O 2 to act as a coreactant for generating reactive oxygen species (ROS, e.g., • OH), thereby oxidizing the substrates to produce analytical signals for reporting the levels of targets. , Nevertheless, the poor stability of H 2 O 2 limits its practical application in onsite food quality and safety analysis. On this background, the self-supplying H 2 O 2 -facilitated cascade catalytic analytical system, which involves natural enzyme-assisted peroxidase-like nanozymes, has become a very promising way to improve the analytical stability of assays. , Recently, the unique light-to-heat photophysical conversion feature has exhibited great potential in developing a portable and low-cost visual sensing platform .…”

Section: Introductionmentioning

confidence: 99%

Dual-Modal Bimetallic Nanozyme-Based Sensing Platform Combining Colorimetric and Photothermal Signal Cascade Catalytic Enhancement for Detection of Hypoxanthine to Judge Meat Freshness

Wu,

Dilinaer,

Nie

et al. 2023

J. Agric. Food Chem.

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Considering the enormous demand for meat in people’s daily lives, the development of efficient meat freshness assays is of great significance for safeguarding food safety. Here, a novel bimetallic nanozyme Fe@CeO2 with high peroxidase-like activity was first synthesized by embedding ferrocenecarboxylic acid (Fc) into hollow CeO2 nanospheres, which combined with xanthine oxidase (XOD) to develop a self-supplying H2O2-facilitated enzymatic cascade catalytic system of XOD + Fe@CeO2, yielding a meat freshness indicator hypoxanthine (Hx)-responsive colorimetric and photothermal dual-mode analytical platform for judging meat freshness upon the assistance of 3,3′,5,5′-tetramethylbenzidine (TMB). Owing to the catalytic activity of XOD to convert Hx into H2O2, Fe@CeO2 rapidly dissociated it into •OH via a peroxidase activity-triggered Fenton-like reaction, emerging a typical enzymatic cascade catalytic reaction. As a result, the colorless TMB was oxidized to be the product of dark-blue oxTMB by •OH, with a chromogenic reaction-driven absorption enhancement at 652 nm, which endowed it with a significant photothermal effect under 660 nm laser irradiation. On this basis, an Hx concentration-dependent colorimetric and photothermal dual-mode signal cascade catalytic enhancement sensing platform was proposed by integrating with a Color Picker App-installed smartphone and a 660 nm laser-equipped handheld thermal imager, achieving the onsite quantitative, reliable, and visual detection of Hx in real meat samples for judging meat freshness with acceptable results. Notably, the colorimetric and photothermal dual-mode signal cascade catalytic enhancement improved not only the reliability but also the sensitivity of the assay, which provided new insights for efficient onsite visual monitoring of meat freshness to safeguard food safety.

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“…1,4 The catalytic activity of nanozymes facilitates the customization of cascade reaction chains for on-demand detection of various target molecules encompassing biological molecules or related small molecules. In recent years, researchers have also explored the use of nanozymes in agriculture and food science 6,7 to detect certain molecules based on their conventional nanozyme-substrate-dependent reactions platform. These are effective, however, the materials that researchers have contrived are not ideal materials, and thus, they may not be directly utilized in agricultural and environmental applications.…”

Section: Introductionmentioning

confidence: 99%

“…Glyphosate is a widely used herbicide in the agricultural industry; detecting its presence in agricultural products can help prevent human damage and protect crop health. 6,7,[9][10][11] In this study, the first example of the organic compoundbased peroxidase-mimicking nanozyme (OC nanozyme) has been presented. The OC nanozyme has peroxidase-like activity with a decent kinetic profile and a considerably high ecofriendly nature as all its constituent materials are organic.…”

Section: Introductionmentioning

confidence: 99%

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Organic compound-based nanozymes for agricultural herbicide detection

Lee,

Kamruzzaman

2023

Nanoscale

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Magnetic three-phase single-drop microextraction for highly sensitive detection of aflatoxin B1 in agricultural product samples based on peroxidase-like spatial network structure

Cited by 9 publications

References 42 publications

Magnetic nanoparticle‐based immunosensors and aptasensors for mycotoxin detection in foodstuffs: An update

Magnetic nanoparticle‐based immunosensors and aptasensors for mycotoxin detection in foodstuffs: An update

Dual-Modal Bimetallic Nanozyme-Based Sensing Platform Combining Colorimetric and Photothermal Signal Cascade Catalytic Enhancement for Detection of Hypoxanthine to Judge Meat Freshness

Organic compound-based nanozymes for agricultural herbicide detection

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