Magnetic solid-phase extraction of high molecular weight peptides using stearic acid–functionalized magnetic hydroxyapatite nanocomposite: determination of some hypothalamic agents in biological samples

Salehpour, Niloofar; Bayatloo, Mohammad Reza; Nojavan, Saeed

doi:10.1007/s00216-021-03725-6

Cited by 9 publications

(3 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Method precision was evaluated by the RSD values (Tables S2 and S3) of the obtained monomer peak area in SEC chromatograms, which were in the range of 3.9−9.1% (n = 3) and comparable with those of other magnetic solid phase extraction-based analyzing methods. 35,36 As to sensitivity (Table S2), LOD values were 0.02−0.04 mg/mL, while LOQ values were 0.04−0.05 mg/mL. The linear range was 0.25−4.00 mg/mL for all IgG fractions, with the corresponding determination coefficients (R 2 ) all greater than 0.98 (Table S2).…”

Section: ■ Results and Discussionmentioning

confidence: 95%

Tractable Method for Rapid Quality Assessment of Therapeutic Antibodies in Harvested Cell Culture Fluid based on FcγRIIIa-Immobilized Magnetic Microspheres

et al. 2022

View full text Add to dashboard Cite

FcγRIIIa-binding affinity is one of the key factors to ensure the efficacy of many antitumor therapeutic antibodies, which should be monitored along with the titer, protein aggregation, and other critical quality attributes. The conventional workflow for the quality assessment of therapeutic antibodies in harvested cell culture fluid (HCCF) is time-consuming and costly nevertheless. In this study, a tractable method was established for rapid quality assessment of a HCCF sample through differentially extracting IgG with different FcγRIIIa affinity levels using FcγRIIIa-immobilized magnetic microspheres, followed by size exclusion chromatography (SEC) to determine the amount and monomer percentage of IgGs in the preceding eluate. FcγRIIIa-immobilized magnetic microspheres with polydopamine (PDA) and hydrophilic dendrimer (PAMAM) coating (denoted as Fe3O4@PDA@PAMAM-FcγRIIIa) were synthesized for the first time as magnetic adsorbents. The PDA cladding endowed the composites with good chemical stability in acidic elution buffer, and the PAMAM dendrimer empowered the composites of high ligand immobilization capacity and hydrophilic surface. The labile FcγRIIIa was immobilized under mild conditions. By directly applying a simple magnetic solid phase extraction procedure to treat HCCF, favored IgG species with high FcγRIIIa affinity would be selectively captured by Fe3O4@PDA@PAMAM-FcγRIIIa composites for subsequent SEC analysis. The monomer peak area value in SEC, which was set as the read-out of the proposed method, correlated directly with the theoretical overall quality of standard-spiked HCCF samples.

show abstract

Section: ■ Results and Discussionmentioning

confidence: 95%

Tractable Method for Rapid Quality Assessment of Therapeutic Antibodies in Harvested Cell Culture Fluid based on FcγRIIIa-Immobilized Magnetic Microspheres

et al. 2022

View full text Add to dashboard Cite

show abstract

“…It can be derived from calcium-rich animal waste such as seashells, cuttlefish bones, and coral. The crystalline nature and chemical structure of HAP make it an effective adsorbent through electrostatic interactions, hydrogen bonding, ion exchange, and the formation of surface complexes [20][21][22][23][24].…”

Section: Introductionmentioning

confidence: 99%

Green magnetic snail shell hydroxyapatite sorbent for reliable solid‐phase extraction of pesticides from water samples

Sghaier

Labidi

Abdallah

et al. 2023

J of Separation Science

View full text Add to dashboard Cite

To address sustainability issues, the green synthesis of nanomaterials has recently received considerable attention. This article addresses a novel and cost‐effective adsorbent for the extraction of eight phenyl‐N‐methylcarbamate insecticides from water samples. We first synthesized a magnetite/hydroxyapatite nanocomposite using snail shell powder via an environmental friendly approach. The morphology and physicochemical properties of magnetic hydroxyapatite were characterized by Fourier transform infrared spectroscopy, energy‐dispersive X‐ray spectroscopy, and scanning electron microscopy. Magnetic extraction parameters were optimized using a Doehlert matrix. Under optimum conditions, the magnetic extraction coupled with a LC–MS method shows good linearity with R2 ≥ 0.9982, suitable intra‐ and interday precision, and limits of detection and quantification in the range of 0.052–0.093 μg/L and 0.11–0.31 μg/L, respectively. Satisfactory relative recoveries of all carbamates were achieved from fortified water samples in the range of 93.89–101.01%.

show abstract

“…Over the years, a number of sample preparation methods have been developed, such as liquid–liquid extraction (LLE) ( Hammad et al, 2021 ), solid-phase extraction (SPE) ( Fedotov et al, 2019 ), and dispersive solid-phase extraction (DSPE) ( Khashaba et al, 2021 ; Salehpour et al, 2021 ) as well as microextraction techniques including dispersive liquid–liquid microextraction (DLLME) ( Rezaee et al, 2006 ; Zuloaga et al, 2015 ; Mansour and Khairy, 2017 ; Wu et al, 2019 ), solid-phase microextraction (SPME) ( Hawthorne et al, 1992 ; Duman et al, 2020 ; Jagirani and Soylak, 2020 ), and single-drop microextraction (SDME) ( Liu and Dasgupta, 1996 ; Choi et al, 2011 ; Delove Tegladza et al, 2020 ). DLLME is one of the most efficient methods for the separation and preconcentration of analytes due to its ease of operation and low cost ( Moslemzadeh et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Synergistic Combination of Facile Thiol-Maleimide Derivatization and Supramolecular Solvent-Based Microextraction for UHPLC–HRMS Analysis of Glutathione in Biofluids

et al. 2021

View full text Add to dashboard Cite

Glutathione (GSH) is the most abundant non-protein thiol in biofluids, enabling diverse physiological functions. Among the proposed methods for GSH detection, ultra-high-performance liquid chromatography (UHPLC) coupled with high-resolution mass spectrometry (HRMS) has the advantages of high sensitivity and efficiency. In this study, a novel analytical method was developed for the determination of GSH using supramolecular solvent (SUPRAS)-based dispersive liquid–liquid microextraction (DLLME) and UHPLC–HRMS. N-Laurylmaleimide was dissolved in tetrahydrofuran, which served three functions: 1) precipitate the proteins present in the biofluid sample, 2) provide a reaction environment for derivatization, and 3) enable the use of SUPRAS as the dispersing agent. Critical parameters were optimized based on single factor testing and response surface methodology. The established method was validated in terms of linearity, accuracy, precision, and successful quantitative analysis of GSH in saliva, urine, and plasma samples. Experimental results showed that SUPRAS as an extraction solvent was particularly suitable for the extraction of GSH from complex matrices. The current study provides a useful tool for accurate measurements of GSH concentrations, which could potentially be used for clinical diagnostics.

show abstract

Magnetic solid-phase extraction of high molecular weight peptides using stearic acid–functionalized magnetic hydroxyapatite nanocomposite: determination of some hypothalamic agents in biological samples

Cited by 9 publications

References 50 publications

Tractable Method for Rapid Quality Assessment of Therapeutic Antibodies in Harvested Cell Culture Fluid based on FcγRIIIa-Immobilized Magnetic Microspheres

Tractable Method for Rapid Quality Assessment of Therapeutic Antibodies in Harvested Cell Culture Fluid based on FcγRIIIa-Immobilized Magnetic Microspheres

Green magnetic snail shell hydroxyapatite sorbent for reliable solid‐phase extraction of pesticides from water samples

Synergistic Combination of Facile Thiol-Maleimide Derivatization and Supramolecular Solvent-Based Microextraction for UHPLC–HRMS Analysis of Glutathione in Biofluids

Contact Info

Product

Resources

About