Magnetic separation as a new method for the extraction of small molecules from biological fluids of humans

Sample Preparation for Measurement of Plasma Mycophenolic Acid Concentrations Using Chromatographically Functionalized Magnetic Micro-Particles

“…Previously, the application of C18-functionalized magnetic particles for the extractive sample preparation of small molecule analytes in the context of quantitative bio-analytical liquid chromatography tandem mass spectrometry (LC-MS/ MS) methods has been proposed. 1,2 These initial reports demonstrated the feasibility of this approach for exemplary analytes to be quantified in serum or urine. The aim of the study described in the present manuscript was to further validate this technology of sample preparation for an additional analyte of clinical relevance and applying an extended evaluation scheme for biomedical analyses.…”

Rapid determination of endogenous cytokinins in plant samples by combination of magnetic solid phase extraction with hydrophilic interaction chromatography–tandem mass spectrometry

“…Hitherto, many studies have reported the application of MSPE in fields of food analysis [19,21], environmental analysis [23][24][25][26], and biological analysis [22] with Fe 3 O 4 particles modified with hydrophobic groups [26], hydrophilic groups [27], ion exchange groups [28], mixed mode groups [23] or even metal ions [29]. However, to our best knowledge, no application of MSPE has been reported to the analysis of CKs in complex plant tissues.…”

“…In recent years, magnetic solid-phase extraction (MSPE) has received much attention in sample pretreatment [19][20][21][22][23]. The technique is based on magnetic or magnetizable adsorbents with high adsorption ability and superparamagnetism.…”

Magnetic beads as an extraction medium for simultaneous quantification of acetaminophen and structurally related compounds in human serum

“…Several types have been described in the literature; some with porous structure to specifically retain target analytes, for example restricted access materials (RAM) or molecular imprinted polymers (MIP); others with very specific functional groups (e.g. immunosorbents), or unspecific materials such as non‐polar coatings (C8, C18,…), ion exchange groups, or moieties that provide aromatic interactions. The extraction procedure is similar to solid‐phase extraction (SPE); i.e., loading, washing, and elution, with the added advantage that the suspended magnetic beads can be handled as a liquid.…”

“…Possible applications of these sample preparation approaches include drugs of abuse testing (DAT) or therapeutic drug monitoring (TDM). Magnetic particles have frequently been used for extraction and pre‐concentration of drugs in complex biological fluids, mostly for extraction of highly hydrophobic analytes using reversed‐phase materials . Only a few studies have reported on the extraction of strongly polar analytes from biological fluids.…”