Magnetic Scaffolds Enriched with Bioactive Nanoparticles for Tissue Engineering

Abstract

Novel magnetic fibrin hydrogel scaffolds for cell implantation and tissue engineering are reported. The magnetic scaffolds are produced by the interaction between thrombin-conjugated maghemite nanoparticles of narrow size distribution and fibrinogen. These scaffolds, enriched with growth factor conjugated fluorescent maghemite nanoparticles, provide a supporting 3D environment for massive proliferation of various cell types, and can be successfully visualized by MRI.

Differentiation Capacity of Monocyte-Derived Multipotential Cells on Nanocomposite Poly(e-caprolactone)-Based Thin Films

“…WJSCs are used as a control cell line in order to compare the differentiation potential of MOMCs with WJSCs respectively. We also use Human Umbilical Vein Endothelial Cell line (HUVEC) [28] to strengthen and accelerate MOMCs differentiation to endothelium given that, in contrast to WJSCs, MOMCs are unable to be maintained in cell culture for a long time [15,29]. The presence of HUVEC in a new semi-permeable co-culture system with MOMCs, ensures that in a limited time this easily collected category of stem cells could be exploited for autologous transplantation of a scaffold for endothelium regeneration [30,31].…”

Injectable In Situ Forming Hybrid Iron Oxide-Hyaluronic Acid Hydrogel for Magnetic Resonance Imaging and Drug Delivery

“…However, these changes are not always reliable because the MR image of water in hydrogel material cannot easily be distinguished from the large water background signal. Therefore, agents such as IONPs, which affect local water relaxation rates, are often required to improve the contrast . The association of IONPs with the hydrogel network is expected to provide correct visualization of the hydrogel implant.…”

“…There have only been a few studies demonstrating the direct labeling of hydrogel scaffolds with IONPs in situ, i.e., during the gelation process . Our group utilized orthogonal chemoselective reactions to form hydrazone cross‐linked HA hydrogel and load it chemically with the model hydrophobic drug (pyrene) via thiol‐cleavable disulfide linkages .…”

“…Therefore, agents such as IONPs, which affect local water relaxation rates, are often required to improve the contrast. [17,18] The association of IONPs with the hydrogel network is expected to provide correct visualization of the hydrogel implant. In this case, degradation of macromolecules within the gel and the release of degradation products will change the concentration and aggregation state of the bound nanoparticles.…”

“…There have only been a few studies demonstrating the direct labeling of hydrogel scaffolds with IONPs in situ, i.e., during the gelation process. [18,19] Our group utilized orthogonal chemoselective reactions to form hydrazone cross-linked HA hydrogel and load it chemically with the model hydrophobic drug (pyrene) via thiol-cleavable disulfide linkages. [20] Interestingly, the resulting hydrogel covalently immobilized with hydrophobic molecules was degraded by the endogenous enzyme hyaluronidase with the formation of nanogel particles.…”