Magnetic Resonance Imaging of Human Tissue-Engineered Adipose Substitutes

Proulx, Maryse; Aubin, Kim; Lagueux, Jean; Audet, Pierre; Auger, Michèle; Fortin, Marc‐André; Fradette, Julie

doi:10.1089/ten.tec.2014.0409

Cited by 8 publications

(7 citation statements)

References 66 publications

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“…Morphological Analysis of the Reconstructed Tissues—Thickness Measurements : The average thickness of each multilayer 3D reconstructed tissue was measured after capturing mosaic images along the entire tissue length of Masson's trichrome‐stained paraffin‐embedded cross sections, as reported previously . The average of six equally distributed tissue thickness measurements were collected, using Axiovision digital image processing software (Zeiss), for each sample in every group from three separate experiments ( n = 2–6 per experiment).…”

Section: Methodsmentioning

confidence: 99%

A Cell‐Based Self‐Assembly Approach for the Production of Human Osseous Tissues from Adipose‐Derived Stromal/Stem Cells

Galbraith

Clafshenkel

Kawecki

et al. 2016

Adv Healthcare Materials

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Achieving optimal bone defect repair is a clinical challenge driving intensive research in the field of bone tissue engineering. Many strategies focus on seeding graft materials with progenitor cells prior to in vivo implantation. Given the benefits of closely mimicking tissue structure and function with natural materials, the authors hypothesize that under specific culture conditions, human adipose-derived stem/stromal cells (hASCs) can solely be used to engineer human reconstructed osseous tissues (hROTs) by undergoing osteoblastic differentiation with concomitant extracellular matrix production and mineralization. Therefore, the authors are developing a self-assembly methodology allowing the production of such osseous tissues. Three-dimensional (3D) tissues reconstructed from osteogenically-induced cell sheets contain abundant collagen type I and are 2.7-fold less contractile compared to non-osteogenically induced tissues. In particular, hROT differentiation and mineralization is reflected by a greater amount of homogenously distributed alkaline phosphatase, as well as higher calcium-containing hydroxyapatite (P < 0.0001) and osteocalcin (P < 0.0001) levels compared to non-induced tissues. Taken together, these findings show that hASC-driven tissue engineering leads to hROTs that demonstrate structural and functional characteristics similar to native osseous tissue. These highly biomimetic human osseous tissues will advantageously serve as a platform for molecular studies as well as for future therapeutic in vivo translation.

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Section: Methodsmentioning

confidence: 99%

A Cell‐Based Self‐Assembly Approach for the Production of Human Osseous Tissues from Adipose‐Derived Stromal/Stem Cells

Galbraith

Clafshenkel

Kawecki

et al. 2016

Adv Healthcare Materials

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Section: Resultsmentioning

confidence: 99%

“…Indeed, among the various strategies aiming at engineering adipose grafts for reconstructive surgery, the use of precursor cells, such as ASCs, that are induced to differentiate into lipid-filled adipocytes is common. [32] A schematic of the in vitro culture experiments is provided in Figure S9a, Supporting Information. First, the viability of ASCs after incubation with USPIONs-PEG (1 mm), d-(+)-gluconic acid δ-lactone (GDL) (0.5 mm), and alginate (1, 2, and 3 wt%), all products and concentrations relevant for the MRI procedures, was measured with the resazurin assay (Section S5, and Figure S10b, Supporting Information).…”

Section: In Vitro Biocompatibility Assays With Hydrogel Products and ...mentioning

confidence: 99%

A Nanoparticle Ink Allowing the High Precision Visualization of Tissue Engineered Scaffolds by MRI

Leon‐Chaviano

Kiseleva

Legros

et al. 2023

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Hydrogels are widely used as cell scaffolds in several biomedical applications. Once implanted in vivo, cell scaffolds must often be visualized, and monitored overtime. However, cell scaffolds appear poorly contrasted in most biomedical imaging modalities such as magnetic resonance imaging (MRI). MRI is the imaging technique of choice for high‐resolution visualization of low‐density, water‐rich tissues. Attempts to enhance hydrogel contrast in MRI are performed with “negative” contrast agents that produce several image artifacts impeding the delineation of the implant's contours. In this study, a magnetic ink based on ultra‐small iron oxide nanoparticles (USPIONs; <5 nm diameter cores) is developed and integrated into biocompatible alginate hydrogel used in cell scaffolding applications. Relaxometric properties of the magnetic hydrogel are measured, as well as biocompatibility and MR‐visibility (T1‐weighted mode; in vitro and in vivo). A 2‐week MR follow‐up study is performed in the mouse model, demonstrating no image artifacts, and the retention of “positive” contrast overtime, which allows very precise delineation of tissue grafts with MRI. Finally, a 3D‐contouring procedure developed to facilitate graft delineation and geometrical conformity assessment is applied on an inverted template alginate pore network. This proof‐of‐concept establishes the possibility to reveal precisely engineered hydrogel structures using this USPIONs ink high‐visibility approach.

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“…Stem cell-derived cellular cultures used in cartilage [29], adipose [30] and heart TE [31] have also successfully used MRI in the development, characterization, and clinical translation of the scaffolding constructs. Furthermore, MRI imaging with superparamagnetic iron oxide nanoparticles as exogenous labeling agents has been used to study different stem cell dynamics for both preclinical and clinical applications [32].…”

Section: Scaffold Tracking Techniquesmentioning

confidence: 99%

In Vivo Tracking of Tissue Engineered Constructs

et al. 2019

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To date, the fields of biomaterials science and tissue engineering have shown great promise in creating bioartificial tissues and organs for use in a variety of regenerative medicine applications. With the emergence of new technologies such as additive biomanufacturing and 3D bioprinting, increasingly complex tissue constructs are being fabricated to fulfill the desired patient-specific requirements. Fundamental to the further advancement of this field is the design and development of imaging modalities that can enable visualization of the bioengineered constructs following implantation, at adequate spatial and temporal resolution and high penetration depths. These in vivo tracking techniques should introduce minimum toxicity, disruption, and destruction to treated tissues, while generating clinically relevant signal-to-noise ratios. This article reviews the imaging techniques that are currently being adopted in both research and clinical studies to track tissue engineering scaffolds in vivo, with special attention to 3D bioprinted tissue constructs.

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Magnetic Resonance Imaging of Human Tissue-Engineered Adipose Substitutes

Cited by 8 publications

References 66 publications

A Cell‐Based Self‐Assembly Approach for the Production of Human Osseous Tissues from Adipose‐Derived Stromal/Stem Cells

A Cell‐Based Self‐Assembly Approach for the Production of Human Osseous Tissues from Adipose‐Derived Stromal/Stem Cells

A Nanoparticle Ink Allowing the High Precision Visualization of Tissue Engineered Scaffolds by MRI

In Vivo Tracking of Tissue Engineered Constructs

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