Magnetic resonance for the assessment of myocardial viability

Isbell, David C.; Kramer, Christopher M.

doi:10.1097/01.hco.0000240584.68720.ae

Cited by 28 publications

(25 citation statements)

References 18 publications

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“…Furthermore, the presence of myocardial necrosis was confirmed after the injection of gadolinium in both groups ( Figure 5A and 5B, right, yellow arrows). The extent of LGE was significantly lower in P2Y 12 −/− mice, as quantified by MRI (P<0.005; Figure 6A). As a consequence of the reduction in platelet accumulation in P2Y 12 −/− mice, no significant difference in the MRI signal after MPIO injection was observed between control-MPIOs (green line) and LIBS-MPIOs (yellow line) in these animals ( Figure 6B).…”

Section: P2y 12 Knockout Mice Demonstrate Reduced Platelet and Neutromentioning

confidence: 99%

“…Additional experiments were performed in P2Y 12 −/− mice to study the extent of myocardial ischemia/reperfusion injury and platelet accumulation in a setting with reliable platelet inhibition. After injection of LIBS-MPIOs, no signal increase was observed in these mice, and a similar effect was seen in animals injected with control-MPIOs ( Figure 5A and 5B).…”

Section: P2y 12 Knockout Mice Demonstrate Reduced Platelet and Neutromentioning

confidence: 99%

“…[1][2][3] Platelet inhibition is a key factor in the management of acute myocardial infarction (AMI), and increasingly potent antiplatelet drugs targeting the platelet ADP receptor P2Y 12 have improved patient outcome. 4,5 In addition, the amount of platelets in the ischemic/reperfused myocardium has an important prognostic value.…”

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confidence: 99%

“…12,13 However, detection of platelets needs a targeted molecular imaging approach. 14 We have previously developed a unique contrast agent that specifically detects activated platelets by targeting a ligand-induced binding site (LIBS) of the activated glycoprotein IIb/IIIa receptor using a single-chain antibody directed against these sites.…”

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confidence: 99%

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Dual-Contrast Molecular Imaging Allows Noninvasive Characterization of Myocardial Ischemia/Reperfusion Injury After Coronary Vessel Occlusion in Mice by Magnetic Resonance Imaging

et al. 2014

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Background-Inflammation and myocardial necrosis play important roles in ischemia/reperfusion injury after coronary artery occlusion and recanalization. The detection of inflammatory activity and the extent of myocardial necrosis itself are of great clinical and prognostic interest. We developed a dual, noninvasive imaging approach using molecular magnetic resonance imaging in an in vivo mouse model of myocardial ischemia and reperfusion. Methods and Results-Ischemia/reperfusion injury was induced in 10-week-old C57BL/6N mice by temporary ligation of the left anterior descending coronary artery. Activated platelets were targeted with a contrast agent consisting of microparticles of iron oxide (MPIOs) conjugated to a single-chain antibody directed against a ligand-induced binding site (LIBS) on activated glycoprotein IIb/IIIa (LIBS-MPIOs). After injection and imaging of LIBS-MPIOs, late gadolinium enhancement was used to depict myocardial necrosis; these imaging experiments were also performed in P2Y 12 −/− mice. All imaging results were correlated to immunohistochemistry findings. Activated platelets were detectable by magnetic resonance imaging via a significant signal effect caused by LIBS-MPIOs in the area of left anterior descending coronary artery occlusion 2 hours after reperfusion. In parallel, late gadolinium enhancement identified the extent of myocardial necrosis. Immunohistochemistry confirmed that LIBS-MPIOs bound significantly to microthrombi in reperfused myocardium. Only background binding was found in P2Y 12 −/− mice. Conclusions-Dual molecular imaging of myocardial ischemia/reperfusion injury allows characterization of platelet-driven inflammation by LIBS-MPIOs and myocardial necrosis by late gadolinium enhancement. This noninvasive imaging strategy is of clinical interest for both diagnostic and prognostic purposes and highlights the potential of molecular magnetic resonance imaging for characterizing ischemia/reperfusion injury. (Circulation. 2014;130:676-687.)

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Section: P2y 12 Knockout Mice Demonstrate Reduced Platelet and Neutromentioning

confidence: 99%

Section: P2y 12 Knockout Mice Demonstrate Reduced Platelet and Neutromentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Dual-Contrast Molecular Imaging Allows Noninvasive Characterization of Myocardial Ischemia/Reperfusion Injury After Coronary Vessel Occlusion in Mice by Magnetic Resonance Imaging

et al. 2014

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“…recently,late enhancement MrI imaging has been investigated in the assessment of viability.T he strengthofthis technique is that transmurality of scar canbeaccurately assessed (31). Although this enables also accurate visualization of non-scarred myocardium, the prediction of wall motion recovery is not straightforward.…”

Section: Regional Left Ventricular Functionmentioning

confidence: 99%