Magnetic resonance colonography in clinical use: How far have we come?

Achiam, Michael Patrick; Thomsen, Henrik S.; Rosenberg, Jacob

doi:10.1080/00365520802647418

Cited by 3 publications

(1 citation statement)

References 61 publications

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“…In this study, we rely on simple, rapid mapping of the distal colon with the cross-sectional imaging modality optical coherence tomography (OCT), which has been miniaturized for the investigation of the distal 30 mm of the mouse colon. Although investigations of disease status in mice using whole-body imaging methods such as magnetic resonance imaging 30 and computed tomography 31 have been conducted, adenomas less than 5 mm in diameter are usually too small to be detected. High-resolution versions of these imaging instruments have met with some success in imaging large adenoma in small animal colon, but remain limited in the ability to detect smaller adenoma, are relatively expensive, and slow.…”

Section: Introductionmentioning

confidence: 99%

Time-serial Assessment of Drug Combination Interventions in a Mouse Model of Colorectal Carcinogenesis Using Optical Coherence Tomography

LeGendre-McGhee

Rice

Wall

et al. 2015

Cancer�Growth�Metastasis

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Optical coherence tomography (OCT) is a high-resolution, nondestructive imaging modality that enables time-serial assessment of adenoma development in the mouse model of colorectal cancer. In this study, OCT was utilized to evaluate the effectiveness of interventions with the experimental antitumor agent α-difluoromethylornithine (DFMO) and a nonsteroidal anti-inflammatory drug sulindac during early [chemoprevention (CP)] and late stages [chemotherapy (CT)] of colon tumorigenesis. Biological endpoints for drug interventions included OCT-generated tumor number and tumor burden. Immunochistochemistry was used to evaluate biochemical endpoints [Ki-67, cleaved caspase-3, cyclooxygenase (COX)-2, β-catenin]. K-Ras codon 12 mutations were studied with polymerase chain reaction-based technique. We demonstrated that OCT imaging significantly correlated with histological analysis of both tumor number and tumor burden for all experimental groups (P < 0.0001), but allows more accurate and full characterization of tumor number and burden growth rate because of its time-serial, nondestructive nature. DFMO alone or in combination with sulindac suppressed both the tumor number and tumor burden growth rate in the CP setting because of DFMO-mediated decrease in cell proliferation (Ki-67, P < 0.001) and K-RAS mutations frequency (P = 0.04). In the CT setting, sulindac alone and DFMO/sulindac combination were effective in reducing tumor number, but not tumor burden growth rate. A decrease in COX-2 staining in DFMO/sulindac CT groups (COX-2, P < 0.01) confirmed the treatment effect. Use of nondestructive OCT enabled repeated, quantitative evaluation of tumor number and burden, allowing changes in these parameters to be measured during CP and as a result of CT. In conclusion, OCT is a robust minimally invasive method for monitoring colorectal cancer disease and effectiveness of therapies in mouse models.

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Section: Introductionmentioning

confidence: 99%