Magnetic properties of PfV encapsulating Ni ions

Abstract

We have investigated the effective synthesis of Pyrococcus furious virus like particle (PfV) and the integration of Ni 2+ ions with PfV. Application of Isopropyl-ǃ-D-1-thiogalactopyranoside (IPTG) during incubation of P. furious causes the marked improvement of yield of P. furious. Using the synthesized P. furious, the spherical structure of PfV is successfully obtained. Concerning magnetism, the native PfV shows the diamagnetic properties. On the contrary, after the integration with Ni 2+ ions, the paramagnetic behavior appears, namely the non-saturated M-H curve with zero remanence and coercivity, and Curie law like M-T curve. The obvious changes in the magnetic properties indicate that the Ni 2+ ions are successfully integrated with PfVs.

Magnetic Interaction in Superparamagnetic Co-Pt Nanoparticles Synthesized in Protein Crystal

“…In this study, we used conventional magnetization measurement techniques for solids on an rPfV crystal. The rPfV crystal was expressed and purified using a previously reported method [17,18]. The crystallization of the rPfV crystal was performed using the hanging-drop vapor diffusion method at 20 C.…”

“…VLPs are composed of proteins that directly capture foreign molecules and have a cage structure into which magnetic nanoparticles can be incorporated [12][13][14]. The Pyrococcus furiosus virus-like particle (PfV) [15,16], recently described as Pyrococcus furiosus encapsulin [14], is a promising candidate for this application [17,18] because of various advantages such as high thermal stability derived from the hyperthermophilic archaea [12,13,19]. The PfV particles are composed of 180 protein subunits and have an icosahedral symmetry.…”